FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_82.341000000053ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_82.341000000053ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73543
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250.00209541735.996191
GTATCAA900.032.4965631
GTACACT300.00511898429.9968241
CCAAACA300.00511898429.9968244
TATCAAC1051.8189894E-1227.8541952
TACGGGC507.0742775E-526.99714315
ACGCAGA1159.094947E-1225.432097
AGAGTAC1201.6370905E-1124.3724211
AACGCAG1201.6370905E-1124.372426
CAGAGTA1201.6370905E-1124.3724210
ATCTCCG1152.5283953E-1023.47577745
CAACGCA1252.910383E-1123.3975245
CGCAGAG1252.910383E-1123.3975248
GCAGAGT1252.910383E-1123.3975249
TCAACGC1252.910383E-1123.3975244
ATCAACG1452.0736479E-1020.1702793
AGTACGG1452.0736479E-1020.17027913
GAGTACG1503.274181E-1019.49793612
GTACGGG1907.605195E-915.39310614
TACGGGG1055.946915E-414.9984132
Produced by FastQC (version 0.11.2)