##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_80.341000000053d5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12818 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.04259634888438 38.0 38.0 38.0 32.0 38.0 2 36.111951942580745 38.0 38.0 38.0 32.0 38.0 3 36.04813543454517 38.0 38.0 38.0 32.0 38.0 4 36.19839288500546 38.0 38.0 38.0 32.0 38.0 5 36.202449680137306 38.0 38.0 38.0 32.0 38.0 6 38.05999375877672 40.0 38.0 40.0 38.0 40.0 7 37.93602746138243 40.0 38.0 40.0 32.0 40.0 8 37.87985645186456 40.0 38.0 40.0 32.0 40.0 9 37.88742393509128 40.0 38.0 40.0 32.0 40.0 10 37.83741613356218 40.0 38.0 40.0 32.0 40.0 11 37.88266500234046 40.0 38.0 40.0 32.0 40.0 12 37.72226556405055 40.0 38.0 40.0 32.0 40.0 13 37.7815571852083 40.0 38.0 40.0 32.0 40.0 14 37.68747074426587 40.0 38.0 40.0 32.0 40.0 15 37.75425183335934 40.0 38.0 40.0 32.0 40.0 16 37.76236542362303 40.0 38.0 40.0 32.0 40.0 17 37.76447183647995 40.0 38.0 40.0 32.0 40.0 18 37.70736464347012 40.0 38.0 40.0 32.0 40.0 19 37.721953502886564 40.0 38.0 40.0 32.0 40.0 20 37.70081135902637 40.0 38.0 40.0 32.0 40.0 21 37.70635044468716 40.0 38.0 40.0 32.0 40.0 22 37.7262443438914 40.0 38.0 40.0 32.0 40.0 23 37.74145732563583 40.0 38.0 40.0 32.0 40.0 24 37.70330784833828 40.0 38.0 40.0 32.0 40.0 25 37.71290372913091 40.0 38.0 40.0 32.0 40.0 26 37.617569043532534 40.0 38.0 40.0 32.0 40.0 27 37.575674832267126 40.0 38.0 40.0 32.0 40.0 28 37.565220783273524 40.0 38.0 40.0 32.0 40.0 29 37.51653924169137 40.0 38.0 40.0 32.0 40.0 30 37.480340146668745 40.0 38.0 40.0 32.0 40.0 31 37.460992354501485 40.0 38.0 40.0 32.0 40.0 32 37.472772663442036 40.0 38.0 40.0 32.0 40.0 33 37.39936027461383 40.0 38.0 40.0 32.0 40.0 34 37.45724762053362 40.0 38.0 40.0 32.0 40.0 35 37.48361678889062 40.0 38.0 40.0 32.0 40.0 36 37.431970666250585 40.0 38.0 40.0 32.0 40.0 37 37.440396317678264 40.0 38.0 40.0 32.0 40.0 38 37.521298174442194 40.0 38.0 40.0 32.0 40.0 39 37.42346699953191 40.0 38.0 40.0 32.0 40.0 40 37.44702761741301 40.0 38.0 40.0 32.0 40.0 41 37.42721173349977 40.0 38.0 40.0 32.0 40.0 42 37.35512560461851 40.0 38.0 40.0 32.0 40.0 43 37.38110469652052 40.0 38.0 40.0 32.0 40.0 44 37.327742237478546 40.0 38.0 40.0 32.0 40.0 45 37.36479950070214 40.0 38.0 40.0 32.0 40.0 46 37.342019035731006 40.0 38.0 40.0 32.0 40.0 47 37.401154626306756 40.0 38.0 40.0 32.0 40.0 48 37.326728038695585 40.0 38.0 40.0 32.0 40.0 49 37.33195506319239 40.0 38.0 40.0 32.0 40.0 50 37.30714620065533 40.0 38.0 40.0 32.0 40.0 51 36.77991886409736 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.021745592674875525 2201 2 -0.8131613473727981 2201 3 0.7681972499382965 2201 4 1.8039796982107674 2201 5 0.6962768754451147 2201 6 1.3301477012158855 2201 7 0.961712753250616 2201 8 0.8527805703805384 2201 9 1.2706045486376496 2201 10 0.12168078684713635 2201 11 1.6990701312628786 2201 12 1.35688896334009 2201 13 1.4357359463205768 2201 14 -0.9081829772953824 2201 15 0.5602218261067264 2201 16 1.2375146320001988 2201 17 1.0298221118298372 2201 18 0.7786844129142452 2201 19 0.3989168972632484 2201 20 -1.7285399505879795 2201 21 0.30020671611164573 2201 22 -0.31611306058767497 2201 23 -2.462108267116946 2201 24 -0.2743895678254802 2201 25 0.04214353673651772 2201 26 -0.013003765265118261 2201 27 1.0339798851582103 2201 28 1.2586622537283247 2201 29 -0.5528103738873469 2201 30 -0.19879152312860526 2201 31 -2.1244127473275185 2201 32 0.5764851325623965 2201 33 -0.22138391861668083 2201 34 -0.37648693052496895 2201 35 1.8018432992411135 2201 36 1.5723269001167992 2201 37 1.5697904617779415 2201 38 1.8090542046370715 2201 39 1.6640829475989918 2201 40 -0.18391496707399568 2201 41 0.922654234049709 2201 42 0.9157081237782947 2201 43 0.4368007828911544 2201 44 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-3.37633978565205 1213 30 -2.288987601559974 1213 31 -6.120491178700071 1213 32 -3.4313580046925054 1213 33 -2.5900113695970717 1213 34 -3.670604577583795 1213 35 -3.355019445856925 1213 36 -2.023751531255755 1213 37 -2.6890330676338294 1213 38 -1.5242791286962643 1213 39 -4.226113130832381 1213 40 -1.5839149670739943 1213 41 -6.234208511048333 1213 42 -2.837233052692291 1213 43 -0.5200619622068814 1213 44 -0.6622506997216746 1213 45 -4.1858032023669125 1213 46 -3.218831207603074 1213 47 -2.5323368758670384 1213 48 -1.4519279158143235 1213 49 -3.6211570934585424 1213 50 -4.424840003372708 1213 51 -4.256844472292848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 5.0 22 8.0 23 14.0 24 27.0 25 55.0 26 56.0 27 99.0 28 141.0 29 172.0 30 216.0 31 252.0 32 319.0 33 397.0 34 478.0 35 590.0 36 788.0 37 1146.0 38 2144.0 39 5908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.7701490208317 23.679488179761254 12.491222594991028 26.05914020441601 2 14.713683882040879 24.73084724606023 40.14666874707443 20.408800124824467 3 17.280386955843348 28.249336870026525 30.738024652831953 23.732251521298174 4 13.559057575284756 20.46341082852239 39.33530972070526 26.642221875487593 5 13.036355125604619 38.321110937743796 35.73100327664222 12.911530660009362 6 28.288344515525043 42.46372288968638 17.1789670775472 12.068965517241379 7 24.020908097987206 33.64019347792167 24.925885473552817 17.413012950538306 8 22.764861912934936 37.76720237166485 21.711655484474957 17.756280230925263 9 22.75706038383523 17.810890934623185 22.53861756904353 36.89343111249805 10 14.495241067249182 31.14370416601654 33.687002652519894 20.674052114214387 11 31.549383679201128 25.175534404743328 27.04790138867218 16.227180527383368 12 18.684662193789983 26.509595880792634 33.070681853643315 21.73506007177407 13 25.081916055546888 23.381182711811515 30.113902324855673 21.422998907785924 14 19.090341706974566 23.05351848962397 28.491184272117337 29.36495553128413 15 21.945701357466064 30.66781089093462 24.30956467467624 23.076923076923077 16 23.115930722421595 31.74442190669371 25.78405367452021 19.355593696364487 17 20.564830706818537 33.25011702293649 27.03229833047277 19.152753939772197 18 21.69605242627555 29.481978467779683 29.130909658293024 19.69105944765174 19 20.83788422530816 29.55999375877672 28.288344515525043 21.313777500390078 20 20.954907161803714 29.325947885785613 28.24153534092682 21.47760961148385 21 21.969105944765175 28.459978155718517 27.20393197066625 22.366983928850054 22 21.774067717272587 30.30113902324856 26.806053986581368 21.11873927289749 23 21.781869246372292 27.95287876423779 28.038695584334526 22.22655640505539 24 22.967701669527226 29.037291309096585 26.493992822593228 21.50101419878296 25 23.100327664222185 27.789046653144016 27.531596192853797 21.579029489779998 26 23.89608363239195 28.623810266812296 27.21953502886566 20.260571071930098 27 22.616632860040568 27.812451240443124 26.314557653300046 23.25635824621626 28 22.64003744733968 28.405367452020595 26.34576376969886 22.608831330940866 29 22.897487907629895 27.78124512404431 28.038695584334526 21.28257138399126 30 24.371976907473865 28.467779684818222 26.82165704478078 20.33858636292713 31 22.38258698704946 28.904665314401623 26.60321422998908 22.10953346855984 32 20.90029645810579 28.795443907005776 27.46918396005617 22.835075674832268 33 22.523014510844124 26.946481510376035 27.64861912934935 22.881884849430488 34 21.844281479169915 27.141519737868624 26.985489155874554 24.028709627086908 35 21.789670775471993 27.094710563270404 28.842253081603996 22.27336557965361 36 22.42159463254798 25.830862849118425 28.740833203307847 23.006709315025745 37 21.095334685598377 26.01029801841161 27.843657356841938 25.050709939148074 38 20.759868934311125 23.96629739428928 32.13449836167889 23.139335309720703 39 21.134342331096896 22.9130909658293 29.66921516617257 26.283351536901232 40 20.50241847402091 22.928694024028708 32.07208612888126 24.496801373069122 41 21.087533156498676 23.685442346699954 30.628803245436103 24.598221251365267 42 21.95350288656577 23.65423623030114 31.268528631611797 23.123732251521297 43 20.08893743173662 22.655640505539086 31.98626930878452 25.26915275393977 44 18.161959744109847 24.551412076767047 31.14370416601654 26.142924013106565 45 18.825089717584646 21.672647838976438 32.68060539865814 26.82165704478078 46 19.566234982056482 24.988297706350444 30.68341394913403 24.762053362459042 47 17.295990014042754 23.52161023560618 32.79762833515369 26.384771415197378 48 16.445623342175068 25.667030738024653 33.17210173193946 24.715244187860822 49 18.66125760649087 23.52941176470588 32.08768918708067 25.721641441722575 50 16.008737712591667 25.432984865033546 32.35294117647059 26.2053362459042 51 17.155562490248087 24.215946325479795 30.542986425339368 28.08550475893275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 2.0 16 3.0 17 2.5 18 2.0 19 1.0 20 0.0 21 1.0 22 2.0 23 3.0 24 4.0 25 2.5 26 4.0 27 7.0 28 15.5 29 24.0 30 61.5 31 99.0 32 213.0 33 327.0 34 509.5 35 692.0 36 1040.5 37 1389.0 38 1704.5 39 2020.0 40 2147.0 41 2274.0 42 2070.5 43 1867.0 44 1469.0 45 1071.0 46 876.0 47 681.0 48 561.0 49 441.0 50 347.5 51 254.0 52 236.0 53 218.0 54 187.0 55 156.0 56 148.5 57 141.0 58 143.5 59 146.0 60 148.0 61 150.0 62 146.0 63 142.0 64 141.5 65 141.0 66 137.0 67 133.0 68 124.0 69 115.0 70 107.0 71 99.0 72 81.5 73 64.0 74 56.5 75 45.0 76 41.0 77 33.0 78 25.0 79 25.0 80 25.0 81 15.5 82 6.0 83 4.5 84 3.0 85 2.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007801529099703542 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 12818.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.516461226400374 #Duplication Level Percentage of deduplicated Percentage of total 1 73.12105195307464 44.25027305351849 2 12.079412143870053 14.620065532844437 3 6.316875080572386 11.468247776564207 4 3.4678355034162696 8.394445311281011 5 1.8048214515921104 5.461070369792479 6 1.2375918525203042 4.49368076142924 7 0.7477117442310172 3.167420814479638 8 0.3738558721155086 1.809954751131222 9 0.23204847234755704 1.2638477141519737 >10 0.6187959262601521 5.070993914807302 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 46 0.3588703385863629 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 31 0.2418474020908098 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG 26 0.2028397565922921 No Hit CAATAATGGGTTAAAAGTGTCCTGAAAACAGCAGTGCTAATGCCTGTCTCT 20 0.15603058199407083 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC 18 0.14042752379466375 No Hit GTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGC 18 0.14042752379466375 No Hit ACTCTGGGTTATTTGTGTTACATGCAAGGGTCCATTTGAAGAGAAGGTTCT 17 0.1326259946949602 No Hit CGTAAAACTTGCAACAACAAAGTAGAGGATGTCTAACTTGTTTTTGCAGGG 17 0.1326259946949602 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 17 0.1326259946949602 No Hit AGTTTTACAATCGCCATAAACTTTTACCTCAACAGAATCTTCTTCACCACC 16 0.12482446559525667 No Hit AGAATGGGTTGAAGTGATTGGAAATGAGAAGTAGGTTCCCAAGCTGTCTCT 15 0.11702293649555313 No Hit GAGAGAAGCATTGTCAGAAACTTCTTTGTGATGATTGCATTCAACTCACGG 14 0.10922140739584958 No Hit ATGGCAGTCAATTAGGAGAACTACAATTCGAAAGTTTCAATGCAGCAGAAG 14 0.10922140739584958 No Hit CTCTAGAAATATGATGGTAAACTATCCCAAACTCCTGTCCTCGTGGAAGAA 14 0.10922140739584958 No Hit GGAAATGGGCATTGAAAGTGACGTAACACTTTATGCTACCGACTTGGATGT 13 0.10141987829614604 No Hit AAGTTTATCTGCCTCCTTTTAGTGTGCATTTATCCTACTAAGCCACCAAGG 13 0.10141987829614604 No Hit CTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAATAGC 13 0.10141987829614604 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.08581682009673897 2 0.0 0.0 0.17163364019347793 3 0.0 0.0 0.26525198938992045 4 0.0 0.0 0.34326728038695586 5 0.0 0.0 0.4134810422842877 6 0.0 0.0 0.4212825713839913 7 0.0 0.0 0.4446871586831019 8 0.0 0.0 0.5305039787798409 9 0.0 0.0 0.6631299734748011 10 0.0 0.0 0.8113590263691683 11 0.0 0.0 0.8659697300670931 12 0.0 0.0 0.8893743173662038 13 0.0 0.0 0.9049773755656109 14 0.0 0.0 0.9127789046653144 15 0.0 0.0 0.9361834919644251 16 0.0 0.0 0.9517865501638321 17 0.0 0.0 0.9595880792635356 18 0.0 0.0 0.9907941956623498 19 0.0 0.0 1.006397253861757 20 0.0 0.0 1.139023248556717 21 0.0 0.0 1.1858324231549384 22 0.0 0.0 1.209237010454049 23 0.0 0.0 1.2560461850522702 24 0.0 0.0 1.2794507723513808 25 0.0 0.0 1.3262599469496021 26 0.0 0.0 1.36526759244812 27 0.0 0.0 1.4666874707442659 28 0.0 0.0 1.599313465439226 29 0.0 0.0 1.856763925729443 30 0.0 0.0 2.278046497113434 31 0.0 0.0 3.0347948197846777 32 0.0 0.0 3.783741613356218 33 0.0 0.0 5.039787798408488 34 0.0 0.0 6.264627867061944 35 0.0 0.0 7.785926041504135 36 0.0 0.0 9.041972226556405 37 0.0 0.0 11.335621781869246 38 0.0 0.0 13.67608051178031 39 0.0 0.0 16.227180527383368 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACA 215 6.5868353E-6 11.511628 45 TACACAT 145 0.0062229503 10.862068 45 CTTATAC 260 5.4054944E-5 9.51923 44 TCTTATA 275 9.993908E-5 9.0 43 CTCTTAT 310 3.6711778E-4 7.983871 42 TCTCTTA 325 0.0053235684 6.923077 41 GTCTCTT 345 0.0095014395 6.5217395 40 >>END_MODULE