FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_80.341000000053d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_80.341000000053d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12818
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC460.3588703385863629No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC310.2418474020908098No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG260.2028397565922921No Hit
CAATAATGGGTTAAAAGTGTCCTGAAAACAGCAGTGCTAATGCCTGTCTCT200.15603058199407083No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC180.14042752379466375No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGC180.14042752379466375No Hit
ACTCTGGGTTATTTGTGTTACATGCAAGGGTCCATTTGAAGAGAAGGTTCT170.1326259946949602No Hit
CGTAAAACTTGCAACAACAAAGTAGAGGATGTCTAACTTGTTTTTGCAGGG170.1326259946949602No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC170.1326259946949602No Hit
AGTTTTACAATCGCCATAAACTTTTACCTCAACAGAATCTTCTTCACCACC160.12482446559525667No Hit
AGAATGGGTTGAAGTGATTGGAAATGAGAAGTAGGTTCCCAAGCTGTCTCT150.11702293649555313No Hit
GAGAGAAGCATTGTCAGAAACTTCTTTGTGATGATTGCATTCAACTCACGG140.10922140739584958No Hit
ATGGCAGTCAATTAGGAGAACTACAATTCGAAAGTTTCAATGCAGCAGAAG140.10922140739584958No Hit
CTCTAGAAATATGATGGTAAACTATCCCAAACTCCTGTCCTCGTGGAAGAA140.10922140739584958No Hit
GGAAATGGGCATTGAAAGTGACGTAACACTTTATGCTACCGACTTGGATGT130.10141987829614604No Hit
AAGTTTATCTGCCTCCTTTTAGTGTGCATTTATCCTACTAAGCCACCAAGG130.10141987829614604No Hit
CTATAAAGACACATGCACACGTATGTTTATTGCGGCACTATTCACAATAGC130.10141987829614604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA2156.5868353E-611.51162845
TACACAT1450.006222950310.86206845
CTTATAC2605.4054944E-59.5192344
TCTTATA2759.993908E-59.043
CTCTTAT3103.6711778E-47.98387142
TCTCTTA3250.00532356846.92307741
GTCTCTT3450.00950143956.521739540
Produced by FastQC (version 0.11.2)