FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_79.341000000053c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_79.341000000053c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516242
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC32430.21388406336191718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3650.038.22060445
CGGGTCG250.00210761635.9989734
TATGCCG4400.032.2149943
CCCACGA4700.030.63742419
CGAGACT4500.030.49912823
GGACGTC300.00514766130.0001329
GCCCACG4850.029.69085318
CGAGCCC4900.029.38788415
CCACGAG5150.028.39724720
ACGAGAC5100.027.79332422
AATCTCG4650.027.57985736
CTCGTAT5250.026.99922839
ATGCCGT5300.026.32000444
TCTCGTA5600.026.11532638
CCGAGCC5700.026.05274814
CACGAGA5600.025.31177721
TCGTATG5800.024.43895540
ATCTCGT6000.023.62432337
CGCGGTT500.002264883422.50009718
GCGGGGT500.002265317422.49935544
Produced by FastQC (version 0.11.2)