FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_77.341000000053af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_77.341000000053af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1677551
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC91430.5450206878956287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10900.042.10861245
TATGCCG12300.037.1328443
ATGCCGT13100.035.0369444
CTCGTAT13050.034.82636639
AATCTCG13100.033.8346936
TCTCGTA13450.033.79063438
GCCCACG14200.032.4841618
TCGTATG13950.032.41821740
CCACGAG14500.032.2747320
CCCACGA14350.032.14172719
CGAGACT13950.032.09564623
ACGAGAC14700.031.68256222
CACGAGA15550.030.24009721
CGACGCG300.005146558430.00162917
GACGCGC300.005146558430.00162918
TACAATC15250.029.35962533
GACTGAT15800.028.33930226
TTACAAT15900.028.01788332
ATCTCGT16600.027.64963237
CGAGCCC16850.027.50891115
Produced by FastQC (version 0.11.2)