FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_76.34100000005392.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_76.34100000005392.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574951
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC70190.445664658773511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8450.040.74027645
TATGCCG9150.037.6235443
ATGCCGT9750.035.3082444
CGAGACT10050.034.25317423
TCTCGTA10150.034.1373838
CTCGTAT10100.034.08360739
CCACGAG10500.033.4280320
CCCACGA10300.033.42178719
ATCTCGT10650.032.9572137
AATCTCG10350.032.60816636
CACGAGA10900.031.78847521
ACGAGAC11050.031.76418922
GCCCACG10850.031.72860318
TCGTATG11100.031.2157140
GTATACG300.005140251530.009041
CGGTAAT453.4844823E-530.009041
GACTGAT11850.028.2906826
TGATTAC12150.027.59214429
CGAGCCC12850.026.790315
AGCCCAC13300.025.8838617
Produced by FastQC (version 0.11.2)