FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_75.34100000005385.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_75.34100000005385.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72175
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT253.8544487E-545.044
CGTATGC301.1293521E-437.49999641
CGGTTTG250.002094074236.014
TATGCCG352.794428E-432.14285743
TCGTATG352.794428E-432.14285740
ACGAGAC352.794428E-432.14285722
CACGAGA300.005115717629.99999821
GGTATCA300.005115717629.9999981
ATCTCGT406.109851E-428.12537
GCCCACG406.109851E-428.12518
CTCGTAT406.109851E-428.12539
TCTCGTA406.109851E-428.12538
AGCCCAC450.001215444225.017
TGCCGTC450.001215444225.045
GTATCAA551.3509268E-424.5454541
CGAGCCC500.002244256722.515
CCGAGCC550.003901435320.45454614
GTATGCC600.006452898518.74999842
CATCTCC2800.017.67857245
TATCAAC850.002534055815.8823532
Produced by FastQC (version 0.11.2)