##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 18h_74.34100000005378.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3807
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.775939059627	38.0	32.0	38.0	32.0	38.0
2	34.96164959285527	38.0	32.0	38.0	32.0	38.0
3	34.60625164171263	38.0	32.0	38.0	32.0	38.0
4	35.1284475965327	38.0	32.0	38.0	32.0	38.0
5	35.225111636459154	38.0	32.0	38.0	32.0	38.0
6	36.7344365642238	38.0	38.0	40.0	32.0	40.0
7	36.37956396112425	38.0	38.0	40.0	32.0	40.0
8	36.16101917520357	38.0	38.0	40.0	32.0	40.0
9	36.16285789335435	38.0	38.0	40.0	27.0	40.0
10	36.19989493039139	38.0	38.0	40.0	27.0	40.0
11	36.20015760441292	38.0	38.0	40.0	27.0	40.0
12	36.16548463356974	38.0	38.0	40.0	32.0	40.0
13	36.179143682689784	38.0	38.0	40.0	32.0	40.0
14	36.17283950617284	38.0	38.0	40.0	32.0	40.0
15	36.066981875492516	38.0	38.0	40.0	32.0	40.0
16	35.996847911741526	38.0	38.0	40.0	27.0	40.0
17	35.88757551878119	38.0	38.0	40.0	27.0	40.0
18	35.95823483057526	38.0	38.0	40.0	27.0	40.0
19	35.98397688468611	38.0	38.0	40.0	27.0	40.0
20	35.93485684265826	38.0	38.0	40.0	27.0	40.0
21	36.01260835303388	38.0	38.0	40.0	27.0	40.0
22	36.10795902285264	38.0	38.0	40.0	32.0	40.0
23	36.04150249540321	38.0	38.0	40.0	27.0	40.0
24	35.87601786183347	38.0	38.0	40.0	27.0	40.0
25	35.98923036511689	38.0	38.0	40.0	27.0	40.0
26	35.74940898345154	38.0	38.0	40.0	27.0	40.0
27	35.69214604675597	38.0	38.0	40.0	27.0	40.0
28	35.78854741266089	38.0	38.0	40.0	27.0	40.0
29	35.61491988442343	38.0	38.0	40.0	27.0	40.0
30	35.53007617546625	38.0	38.0	40.0	27.0	40.0
31	35.58234830575256	38.0	38.0	40.0	27.0	40.0
32	35.56553716837405	38.0	38.0	40.0	27.0	40.0
33	35.564223798266354	38.0	38.0	40.0	27.0	40.0
34	35.59311794063567	38.0	38.0	40.0	27.0	40.0
35	35.66246388232204	38.0	38.0	40.0	27.0	40.0
36	35.594956658786444	38.0	38.0	40.0	27.0	40.0
37	35.6267402153927	38.0	38.0	40.0	27.0	40.0
38	35.6976622012083	38.0	38.0	40.0	27.0	40.0
39	35.44943525085369	38.0	38.0	40.0	27.0	40.0
40	35.613606514315734	38.0	38.0	40.0	27.0	40.0
41	35.670344102968215	38.0	38.0	40.0	27.0	40.0
42	35.60388757551878	38.0	38.0	40.0	27.0	40.0
43	35.65668505384817	38.0	38.0	40.0	27.0	40.0
44	35.52061991069083	38.0	38.0	40.0	27.0	40.0
45	35.76438140267928	38.0	38.0	40.0	27.0	40.0
46	35.545573942737064	38.0	38.0	40.0	27.0	40.0
47	35.44575781455214	38.0	32.0	40.0	27.0	40.0
48	35.45232466509062	38.0	38.0	40.0	27.0	40.0
49	35.30864197530864	38.0	32.0	40.0	27.0	40.0
50	35.54242185447859	38.0	38.0	40.0	27.0	40.0
51	34.6193853427896	38.0	32.0	38.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	1.711662946428575
2201	2	2.5027715773809547
2201	3	1.4807291666666629
2201	4	0.7849516369047578
2201	5	-0.6954055059523796
2201	6	1.9241815476190567
2201	7	2.783537946428588
2201	8	2.703311011904759
2201	9	3.03803943452381
2201	10	2.4671874999999943
2201	11	2.1097656250000085
2201	12	1.9088727678571473
2201	13	-0.03530505952380736
2201	14	0.40589657738096463
2201	15	1.5543712797619023
2201	16	2.950502232142867
2201	17	-2.417020089285721
2201	18	-3.739936755952378
2201	19	-3.1419642857142804
2201	20	-0.012779017857148744
2201	21	2.542485119047612
2201	22	3.824107142857116
2201	23	2.5924293154761884
2201	24	3.4640438988095283
2201	25	2.790290178571439
2201	26	1.5595052083333343
2201	27	1.5429315476190624
2201	28	2.3199776785714192
2201	29	1.8325148809523881
2201	30	3.839676339285724
2201	31	2.072451636904759
2201	32	-1.5230282738095227
2201	33	1.7223400297619094
2201	34	2.899944196428585
2201	35	2.554241071428571
2201	36	0.5255580357142904
2201	37	0.8297619047619094
2201	38	2.55585937499999
2201	39	2.058426339285724
2201	40	1.0058221726190482
2201	41	1.7627790178571345
2201	42	-0.7289062500000014
2201	43	1.0323474702380793
2201	44	1.8888764880952493
2201	45	2.3956845238095212
2201	46	3.0882812500000085
2201	47	3.3030691964285737
2201	48	2.9188802083333414
2201	49	2.731938244047633
2201	50	3.1109561011904745
2201	51	3.6168898809523853
1101	1	-1.8883370535714263
1101	2	0.1027715773809561
1101	3	2.28072916666666
1101	4	-4.615048363095241
1101	5	0.10459449404761756
1101	6	0.9241815476190567
1101	7	1.1835379464285865
1101	8	2.969977678571425
1101	9	1.7047061011904745
1101	10	-2.5994791666666686
1101	11	0.70976562500001
1101	12	0.04220610119048018
1101	13	0.0980282738095255
1101	14	1.4058965773809646
1101	15	2.4210379464285694
1101	16	1.2171688988095326
1101	17	2.5496465773809405
1101	18	1.2600632440476218
1101	19	1.1247023809523853
1101	20	-2.7127790178571516
1101	21	0.5424851190476119
1101	22	-1.6425595238095454
1101	23	-2.6075706845238145
1101	24	1.797377232142864
1101	25	3.056956845238105
1101	26	2.4261718750000014
1101	27	0.34293154761905953
1101	28	0.053311011904753514
1101	29	3.032514880952391
1101	30	2.573009672619058
1101	31	-2.4608816964285793
1101	32	1.0436383928571402
1101	33	1.8890066964285737
1101	34	2.3666108630952465
1101	35	1.0875744047619094
1101	36	2.992224702380952
1101	37	-2.3702380952380935
1101	38	-3.0441406250000114
1101	39	-1.141573660714279
1101	40	1.1058221726190496
1101	41	1.0294456845238003
1101	42	1.7710937499999986
1101	43	1.5323474702380793
1101	44	3.088876488095252
1101	45	-0.20431547619048018
1101	46	1.68828125000001
1101	47	2.769735863095235
1101	48	3.1188802083333442
1101	49	2.931938244047636
1101	50	2.9776227678571416
1101	51	4.150223214285724
2202	1	-4.6883370535714235
2202	2	1.3027715773809518
2202	3	-3.9192708333333357
2202	4	2.7849516369047578
2202	5	1.1045944940476176
2202	6	0.9241815476190567
2202	7	2.9835379464285907
2202	8	2.5699776785714263
2202	9	2.5047061011904717
2202	10	1.2005208333333286
2202	11	-3.0902343749999943
2202	12	-2.95779389880952
2202	13	0.8980282738095298
2202	14	-0.1941034226190368
2202	15	0.821037946428568
2202	16	-0.9828311011904702
2202	17	-4.050353422619054
2202	18	0.46006324404762466
2202	19	1.1247023809523853
2202	20	0.6872209821428541
2202	21	2.342485119047609
2202	22	2.957440476190449
2202	23	0.7924293154761912
2202	24	-3.202622767857136
2202	25	-2.7430431547618994
2202	26	0.4261718750000014
2202	27	1.5429315476190624
2202	28	1.2533110119047564
2202	29	1.4325148809523895
2202	30	2.573009672619058
2202	31	-2.860881696428578
2202	32	2.443638392857139
2202	33	-2.310993303571429
2202	34	-4.433389136904751
2202	35	1.8875744047619065
2202	36	1.792224702380956
2202	37	2.229761904761908
2202	38	2.355859374999987
2202	39	1.2584263392857267
2202	40	2.705822172619051
2202	41	0.229445684523796
2202	42	0.5710937499999957
2202	43	1.9323474702380778
2202	44	1.0888764880952522
2202	45	-0.20431547619048018
2202	46	1.2882812500000114
2202	47	-2.230264136904765
2202	48	0.31888020833333997
2202	49	1.7319382440476332
2202	50	-0.02237723214285836
2202	51	-1.6497767857142804
1102	1	2.711662946428575
1102	2	1.5027715773809547
1102	3	-1.6859375000000014
1102	4	-0.21504836309524222
1102	5	2.3045944940476204
1102	6	-2.5758184523809433
1102	7	1.7835379464285879
1102	8	1.0366443452380878
1102	9	-1.4619605654761898
1102	10	1.80052083333333
1102	11	-2.3902343749999915
1102	12	1.242206101190483
1102	13	-1.868638392857136
1102	14	2.072563244047629
1102	15	0.05437127976190226
1102	16	1.2838355654761955
1102	17	-2.417020089285721
1102	18	-0.23993675595237818
1102	19	-2.308630952380952
1102	20	-2.679445684523813
1102	21	0.3758184523809476
1102	22	2.1574404761904518
1102	23	0.9257626488095241
1102	24	0.46404389880952834
1102	25	-0.8763764880952323
1102	26	-2.9404947916666657
1102	27	0.8762648809523981
1102	28	-2.1800223214285808
1102	29	-8.184523809475763E-4
1102	30	1.8396763392857238
1102	31	0.4057849702380878
1102	32	0.14363839285714164
1102	33	1.8890066964285737
1102	34	2.2332775297619136
1102	35	2.8875744047619065
1102	36	-0.3077752976190453
1102	37	3.163095238095245
1102	38	-0.94414062500001
1102	39	2.058426339285724
1102	40	3.1724888392857125
1102	41	2.096112351190463
1102	42	2.437760416666663
1102	43	1.1990141369047436
1102	44	1.8888764880952493
1102	45	2.0623511904761855
1102	46	1.0882812500000085
1102	47	2.969735863095238
1102	48	2.9188802083333414
1102	49	0.8986049107142975
1102	50	-1.055710565476197
1102	51	0.11688988095238528
2203	1	0.7116629464285751
2203	2	1.5027715773809547
2203	3	1.4807291666666629
2203	4	0.9516183035714221
2203	5	2.3045944940476204
2203	6	0.9241815476190567
2203	7	2.783537946428588
2203	8	2.703311011904759
2203	9	3.03803943452381
2203	10	2.1338541666666657
2203	11	2.776432291666673
2203	12	1.5755394345238187
2203	13	2.9646949404761926
2203	14	2.4058965773809646
2203	15	2.2210379464285666
2203	16	3.2838355654761955
2203	17	2.082979910714279
2203	18	-0.23993675595237818
2203	19	2.524702380952384
2203	20	1.4872209821428513
2203	21	1.5424851190476119
2203	22	-0.6758928571428839
2203	23	1.0924293154761884
2203	24	-1.0359561011904717
2203	25	1.2902901785714391
2203	26	2.5595052083333343
2203	27	0.8762648809523981
2203	28	2.9866443452380906
2203	29	2.165848214285724
2203	30	2.1730096726190595
2203	31	2.072451636904759
2203	32	1.9769717261904773
2203	33	2.2223400297619094
2203	34	1.899944196428585
2203	35	1.8875744047619065
2203	36	3.1922247023809547
2203	37	2.8297619047619094
2203	38	2.55585937499999
2203	39	2.058426339285724
2203	40	1.8391555059523839
2203	41	-1.7372209821428655
2203	42	1.7710937499999986
2203	43	2.5323474702380793
2203	44	2.222209821428585
2203	45	1.3956845238095212
2203	46	-0.07838541666665577
2203	47	0.8030691964285737
2203	48	-5.7477864583333265
2203	49	-1.434728422619031
2203	50	0.6109561011904745
2203	51	0.450223214285721
1103	1	0.2830915178571445
1103	2	0.7884858630952394
1103	3	1.7664434523809476
1103	4	2.7849516369047578
1103	5	0.5903087797619051
1103	6	1.495610119047626
1103	7	2.9263950892857338
1103	8	1.0842633928571388
1103	9	1.7047061011904745
1103	10	1.3719494047618994
1103	11	1.1097656250000085
1103	12	2.099348958333337
1103	13	1.8694568452380977
1103	14	2.2630394345238187
1103	15	2.935323660714282
1103	16	1.9028831845238159
1103	17	2.035360863095228
1103	18	2.4029203869047677
1103	19	1.8104166666666686
1103	20	2.0586495535714207
1103	21	1.2567708333333272
1103	22	2.4431547619047365
1103	23	1.3067150297619037
1103	24	1.3688058035714334
1103	25	1.1712425595238187
1103	26	2.0833147321428527
1103	27	2.4000744047619165
1103	28	2.796168154761901
1103	29	3.2610863095238187
1103	30	2.744438244047629
1103	31	2.5962611607142776
1103	32	1.9293526785714263
1103	33	1.603292410714289
1103	34	-0.7191034226190354
1103	35	1.6018601190476218
1103	36	1.90651041666667
1103	37	1.5440476190476247
1103	38	2.1272879464285595
1103	39	2.915569196428578
1103	40	2.0772507440476176
1103	41	2.0008742559523682
1103	42	1.4853794642857139
1103	43	-1.181938244047636
1103	44	2.7460193452381034
1103	45	2.2528273809523753
1103	46	3.516852678571439
1103	47	2.826878720238092
1103	48	1.9188802083333414
1103	49	1.7319382440476332
1103	50	2.349051339285708
1103	51	3.2359375000000057
2204	1	-0.12167038690476062
2204	2	0.669438244047619
2204	3	-3.685937500000005
2204	4	-2.2150483630952422
2204	5	-1.862072172619044
2204	6	-2.909151785714279
2204	7	-1.7164620535714121
2204	8	1.0366443452380878
2204	9	-0.9619605654761898
2204	10	-6.866145833333338
2204	11	-5.556901041666659
2204	12	-5.091127232142849
2204	13	-3.2019717261904717
2204	14	-7.594103422619035
2204	15	-9.612295386904766
2204	16	-1.8828311011904688
2204	17	-3.5836867559523924
2204	18	-5.07327008928571
2204	19	-8.308630952380948
2204	20	-2.8461123511904844
2204	21	-8.457514880952388
2204	22	-9.67589285714288
2204	23	-5.240904017857144
2204	24	-3.8692894345238003
2204	25	-0.20970982142856087
2204	26	-3.607161458333337
2204	27	-2.9570684523809376
2204	28	-1.1800223214285808
2204	29	-3.0008184523809476
2204	30	-11.82699032738094
2204	31	-3.094215029761912
2204	32	-1.0230282738095227
2204	33	-1.6109933035714263
2204	34	-3.4333891369047507
2204	35	-3.945758928571429
2204	36	-3.8077752976190453
2204	37	-0.6702380952380906
2204	38	0.8891927083333258
2204	39	-4.108240327380944
2204	40	-2.3275111607142875
2204	41	-3.403887648809537
2204	42	-6.228906250000001
2204	43	0.03234747023807927
2204	44	-1.9444568452380864
2204	45	-1.1043154761904788
2204	46	-0.9117187499999915
2204	47	-4.19693080357143
2204	48	-5.2477864583333265
2204	49	-3.934728422619031
2204	50	-0.8890438988095255
2204	51	-9.216443452380947
2205	1	1.711662946428575
2205	2	1.5027715773809547
2205	3	3.480729166666663
2205	4	0.7849516369047578
2205	5	0.3045944940476204
2205	6	2.2575148809523924
2205	7	2.450204613095252
2205	8	3.3699776785714235
2205	9	2.7047061011904745
2205	10	2.4671874999999943
2205	11	2.4430989583333442
2205	12	-1.9244605654761813
2205	13	-0.5353050595238074
2205	14	-0.09410342261903537
2205	15	1.2210379464285666
2205	16	1.6171688988095312
2205	17	-1.7503534226190567
2205	18	1.5933965773809575
2205	19	2.8580357142857196
2205	20	1.1538876488095156
2205	21	1.5424851190476119
2205	22	3.4907738095237875
2205	23	2.5924293154761884
2205	24	1.4640438988095283
2205	25	-2.3763764880952323
2205	26	-2.9404947916666657
2205	27	2.5429315476190624
2205	28	0.48664434523809064
2205	29	2.4991815476190524
2205	30	-0.9936569940476048
2205	31	0.5724516369047592
2205	32	1.6436383928571416
2205	33	2.8890066964285737
2205	34	2.899944196428585
2205	35	0.7209077380952422
2205	36	1.358891369047619
2205	37	-0.6702380952380906
2205	38	-1.6108072916666742
2205	39	1.5584263392857238
2205	40	-1.9941778273809518
2205	41	0.5961123511904631
2205	42	1.7710937499999986
2205	43	0.6990141369047436
2205	44	1.5555431547619136
2205	45	1.0623511904761855
2205	46	2.754947916666673
2205	47	1.969735863095238
2205	48	2.5855468750000057
2205	49	2.731938244047633
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1211	28	3.153311011904755
1211	29	2.332514880952388
1211	30	1.6730096726190595
1211	31	2.9891183035714235
1211	32	2.1436383928571416
1211	33	2.6390066964285737
1211	34	-0.05838913690475067
1211	35	-0.23742559523809348
1211	36	2.0672247023809547
1211	37	1.7047619047619094
1211	38	1.18085937499999
1211	39	2.558426339285724
1211	40	2.255822172619048
1211	41	2.179445684523799
1211	42	2.5210937499999986
1211	43	2.2823474702380793
1211	44	0.013876488095249329
1211	45	1.8956845238095212
1211	46	-0.036718749999991473
1211	47	1.844735863095238
1211	48	-0.7061197916666586
1211	49	1.1069382440476332
1211	50	1.2776227678571388
1211	51	2.450223214285721
1208	1	1.961662946428575
1208	2	-1.1222284226190453
1208	3	0.4807291666666629
1208	4	1.2849516369047578
1208	5	0.1795944940476204
1208	6	-2.2008184523809433
1208	7	-2.841462053571412
1208	8	1.1199776785714235
1208	9	-2.4202938988095255
1208	10	-0.82447916666667
1208	11	-2.8902343749999915
1208	12	-5.882793898809517
1208	13	-2.4519717261904717
1208	14	0.7808965773809646
1208	15	1.2210379464285666
1208	16	0.8671688988095312
1208	17	-0.6253534226190567
1208	18	1.0100632440476218
1208	19	0.8997023809523839
1208	20	2.4872209821428513
1208	21	1.1674851190476119
1208	22	0.5324404761904518
1208	23	-2.1575706845238116
1208	24	1.547377232142864
1208	25	-2.6680431547618966
1208	26	-0.6488281250000014
1208	27	1.4179315476190624
1208	28	-0.22168898809524507
1208	29	-4.167485119047612
1208	30	2.1730096726190595
1208	31	0.2391183035714235
1208	32	0.26863839285714164
1208	33	-6.360993303571426
1208	34	-1.5583891369047507
1208	35	0.2625744047619065
1208	36	2.4422247023809547
1208	37	1.8297619047619094
1208	38	-0.81914062500001
1208	39	-3.441573660714276
1208	40	1.5058221726190482
1208	41	-0.07055431547620117
1208	42	-0.3539062500000014
1208	43	2.2823474702380793
1208	44	0.013876488095249329
1208	45	0.7706845238095212
1208	46	-0.036718749999991473
1208	47	-0.655264136904762
1208	48	3.1688802083333414
1208	49	-5.018061755952367
1208	50	-1.5973772321428612
1208	51	-2.799776785714279
1209	1	1.711662946428575
1209	2	1.5027715773809547
1209	3	2.480729166666663
1209	4	2.7849516369047578
1209	5	2.3045944940476204
1209	6	2.590848214285721
1209	7	2.450204613095252
1209	8	2.703311011904759
1209	9	2.7047061011904745
1209	10	1.80052083333333
1209	11	2.4430989583333442
1209	12	2.242206101190483
1209	13	1.9646949404761926
1209	14	2.7392299107143003
1209	15	2.5543712797619023
1209	16	2.2838355654761955
1209	17	3.082979910714279
1209	18	2.926729910714286
1209	19	2.524702380952384
1209	20	2.1538876488095156
1209	21	2.542485119047612
1209	22	3.4907738095237875
1209	23	1.2590959821428527
1209	24	3.1307105654761997
1209	25	2.790290178571439
1209	26	2.5595052083333343
1209	27	3.2095982142857267
1209	28	2.653311011904755
1209	29	2.832514880952388
1209	30	2.839676339285724
1209	31	3.405784970238088
1209	32	2.6436383928571416
1209	33	3.2223400297619094
1209	34	2.899944196428585
1209	35	3.554241071428571
1209	36	3.5255580357142904
1209	37	3.4964285714285737
1209	38	2.2225260416666544
1209	39	3.725093005952388
1209	40	2.839155505952384
1209	41	2.429445684523799
1209	42	3.1044270833333343
1209	43	2.5323474702380793
1209	44	3.5555431547619136
1209	45	2.729017857142857
1209	46	3.0882812500000085
1209	47	3.969735863095238
1209	48	2.5855468750000057
1209	49	2.3986049107142975
1209	50	3.444289434523803
1209	51	3.2835565476190567
1214	1	-0.6883370535714235
1214	2	-2.0972284226190467
1214	3	-1.5192708333333371
1214	4	-2.2150483630952422
1214	5	-3.895405505952379
1214	6	-0.4758184523809419
1214	7	-2.0164620535714093
1214	8	-4.430022321428574
1214	9	-0.49529389880952834
1214	10	-0.9994791666666671
1214	11	2.3097656250000114
1214	12	0.8422061011904844
1214	13	2.0980282738095255
1214	14	-1.5941034226190354
1214	15	-6.778962053571433
1214	16	-4.782831101190467
1214	17	-2.2503534226190567
1214	18	0.46006324404762466
1214	19	0.7247023809523867
1214	20	0.6872209821428541
1214	21	-5.457514880952388
1214	22	-2.042559523809551
1214	23	-0.20757068452380878
1214	24	1.797377232142864
1214	25	2.6569568452381063
1214	26	0.826171875
1214	27	-0.45706845238093763
1214	28	1.653311011904755
1214	29	2.6325148809523853
1214	30	1.773009672619061
1214	31	2.5391183035714207
1214	32	2.8436383928571445
1214	33	1.0890066964285765
1214	34	0.7666108630952522
1214	35	0.48757440476190794
1214	36	2.5922247023809533
1214	37	2.229761904761908
1214	38	-3.0441406250000114
1214	39	-2.5415736607142776
1214	40	0.10582217261904958
1214	41	-2.3705543154761983
1214	42	0.37109375
1214	43	2.3323474702380764
1214	44	0.2888764880952479
1214	45	0.5956845238095241
1214	46	1.2882812500000114
1214	47	-0.4302641369047606
1214	48	2.7188802083333385
1214	49	2.931938244047636
1214	50	-3.62237723214286
1214	51	-4.249776785714278
1215	1	-2.455003720238089
1215	2	-4.663895089285713
1215	3	0.4807291666666629
1215	4	1.7849516369047578
1215	5	-0.6954055059523796
1215	6	0.2575148809523924
1215	7	1.4502046130952522
1215	8	1.0366443452380878
1215	9	1.0380394345238102
1215	10	-0.8661458333333343
1215	11	-0.22356770833332718
1215	12	-0.257793898809517
1215	13	-2.2019717261904717
1215	14	-2.0941034226190354
1215	15	1.5543712797619023
1215	16	-1.8828311011904688
1215	17	-2.0836867559523924
1215	18	-1.2399367559523782
1215	19	-4.14196428571428
1215	20	0.32055431547618696
1215	21	-1.9575148809523881
1215	22	-0.009226190476212537
1215	23	2.2590959821428527
1215	24	1.4640438988095283
1215	25	1.1236235119047677
1215	26	0.5595052083333343
1215	27	0.20959821428572667
1215	28	1.9866443452380906
1215	29	0.16584821428572383
1215	30	3.1730096726190595
1215	31	1.4057849702380878
1215	32	-1.8563616071428584
1215	33	-3.6109933035714263
1215	34	0.23327752976191363
1215	35	2.2209077380952422
1215	36	2.858891369047619
1215	37	2.163095238095245
1215	38	1.2225260416666544
1215	39	2.725093005952388
1215	40	-0.16084449404761614
1215	41	0.26277901785713453
1215	42	-1.3955729166666657
1215	43	-1.4676525297619207
1215	44	-1.9444568452380864
1215	45	1.0623511904761855
1215	46	0.9216145833333442
1215	47	-1.1969308035714263
1215	48	-0.5811197916666586
1215	49	0.2319382440476332
1215	50	-2.055710565476197
1215	51	1.6168898809523853
1212	1	1.854520089285721
1212	2	1.6456287202381006
1212	3	1.7664434523809476
1212	4	1.9278087797619037
1212	5	0.5903087797619051
1212	6	0.4956101190476261
1212	7	1.640680803571442
1212	8	1.3699776785714235
1212	9	2.5618489583333286
1212	10	1.6576636904761841
1212	11	-1.6045200892857068
1212	12	1.242206101190483
1212	13	2.1551711309523824
1212	14	1.977325148809534
1212	15	0.9353236607142819
1212	16	1.9028831845238159
1212	17	-1.821781994047626
1212	18	0.2600632440476218
1212	19	1.5247023809523839
1212	20	-1.227064732142864
1212	21	-0.028943452380957524
1212	22	1.871726190476167
1212	23	2.7352864583333343
1212	24	1.0830915178571487
1212	25	1.1712425595238187
1212	26	1.5118861607142833
1212	27	2.4000744047619165
1212	28	2.796168154761901
1212	29	2.6896577380952422
1212	30	2.4587239583333442
1212	31	1.7391183035714235
1212	32	2.215066964285711
1212	33	1.8890066964285737
1212	34	2.1380394345238187
1212	35	2.173288690476191
1212	36	0.04936755952380878
1212	37	0.25833333333333997
1212	38	0.698716517857136
1212	39	2.3441406250000085
1212	40	1.5058221726190482
1212	41	2.8580171130952294
1212	42	2.6282366071428527
1212	43	-2.324795386904775
1212	44	-0.825409226190466
1212	45	-0.17574404761904816
1212	46	2.9454241071428626
1212	47	2.826878720238092
1212	48	2.490308779761911
1212	49	3.160509672619064
1212	50	2.0633370535714235
1212	51	0.23593750000000568
1213	1	0.3116629464285765
1213	2	0.1027715773809561
1213	3	-0.11927083333333854
1213	4	1.584951636904755
1213	5	-2.6954055059523796
1213	6	-0.07581845238094331
1213	7	-3.6164620535714107
1213	8	0.9699776785714249
1213	9	2.5047061011904717
1213	10	0.40052083333333144
1213	11	1.509765625000007
1213	12	0.8422061011904844
1213	13	0.8980282738095298
1213	14	0.0058965773809660504
1213	15	2.0210379464285637
1213	16	-0.18283110119046597
1213	17	1.3496465773809447
1213	18	-0.3399367559523796
1213	19	-1.4752976190476161
1213	20	2.2872209821428484
1213	21	-2.657514880952391
1213	22	-7.0425595238095475
1213	23	0.1924293154761898
1213	24	-8.602622767857135
1213	25	-2.3430431547618937
1213	26	0.826171875
1213	27	-3.857068452380936
1213	28	0.25331101190475636
1213	29	-2.3674851190476147
1213	30	-4.226990327380939
1213	31	1.339118303571425
1213	32	-2.95636160714286
1213	33	-2.310993303571429
1213	34	-3.4333891369047507
1213	35	-0.7124255952380949
1213	36	-0.8077752976190453
1213	37	-0.7702380952380921
1213	38	-2.644140625000013
1213	39	-3.3415736607142748
1213	40	0.10582217261904958
1213	41	-2.770554315476204
1213	42	1.37109375
1213	43	1.1323474702380807
1213	44	-2.3111235119047535
1213	45	2.1956845238095184
1213	46	-2.1117187499999943
1213	47	-3.4302641369047606
1213	48	-1.8811197916666558
1213	49	0.931938244047636
1213	50	2.577622767857136
1213	51	-1.2497767857142819
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	0.0
23	18.0
24	19.0
25	41.0
26	35.0
27	63.0
28	103.0
29	124.0
30	123.0
31	168.0
32	177.0
33	180.0
34	236.0
35	303.0
36	322.0
37	447.0
38	627.0
39	819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.89069889647924	17.288491854965844	13.846558066211244	32.974251182343664
2	14.342001576044131	21.35539795114263	40.031520882584715	24.271079590228524
3	16.86367218282112	22.642500656685055	33.46467034410297	27.02915681639086
4	12.266876805883898	20.85631731021802	36.87943262411347	29.99737325978461
5	12.634620436038876	35.04071447333858	36.56422379826635	15.760441292356187
6	29.13054898870502	41.108484370895724	15.99684791174153	13.764118728657737
7	25.08536905700026	31.12687155240347	22.327291830837932	21.46046755975834
8	22.826372471762543	38.61308116627266	20.751247701602313	17.80929866036249
9	24.717625426845284	16.86367218282112	19.17520357236669	39.2434988179669
10	14.49960598896769	33.35960073548726	29.498292618859995	22.642500656685055
11	31.337010769634883	22.379826635145783	27.81717888100867	18.465983714210665
12	21.40793275545049	24.980299448384553	29.655897031783557	23.955870764381405
13	26.47754137115839	20.77751510375624	30.049908064092463	22.69503546099291
14	18.728657735749934	21.066456527449436	28.132387706855795	32.07249802994484
15	22.195954820068295	27.554504859469397	23.22038350407145	27.02915681639086
16	26.2936695560809	26.635145784081953	25.584449697924878	21.486734961912266
17	20.98765432098765	29.682164433937487	25.97846073023378	23.35172051484108
18	26.109797741003415	25.558182295770948	27.081691620698713	21.250328342526924
19	22.800105069608616	26.582610979774103	25.374310480693456	25.242973469923825
20	21.98581560283688	26.16233254531127	25.66325190438666	26.188599947465196
21	20.98765432098765	26.188599947465196	25.689519306540582	27.13422642500657
22	21.82821118991332	30.811662726556342	26.661413186235883	20.698712897294456
23	21.670606776989757	26.05726293669556	25.53191489361702	26.740215392697664
24	25.321775676385606	26.136065143157346	26.582610979774103	21.959548200682953
25	21.933280798529026	29.971105857630683	26.267402153926977	21.82821118991332
26	25.269240872077752	26.31993695823483	27.76464407670081	20.646178092986602
27	22.537431048069344	29.86603624901497	25.899658523772	21.696874179143684
28	19.779353821907016	27.29183083793013	26.37247176254268	26.556343577620172
29	22.5899658523772	28.211189913317575	28.368794326241137	20.830049908064094
30	23.903335960073548	27.02915681639086	26.766482794851587	22.301024428684002
31	23.824533753611767	25.847123719464143	28.132387706855795	22.195954820068295
32	21.933280798529026	27.948515891778303	25.426845285001313	24.691358024691358
33	22.169687417914368	26.661413186235883	28.289992119779356	22.878907276070397
34	22.800105069608616	25.558182295770948	28.55266614131862	23.08904649330181
35	22.406094037299713	24.271079590228524	29.83976884686105	23.483057525610718
36	22.983976884686104	24.297346992382455	28.60520094562648	24.113475177304963
37	21.224060940372997	26.608878381928026	27.475702653007616	24.691358024691358
38	18.728657735749934	23.062779091147885	34.672970843183606	23.53559232991857
39	19.753086419753085	21.644339374835827	30.627790911478854	27.97478329393223
40	20.094562647754138	21.98581560283688	34.988179669030735	22.93144208037825
41	19.12266876805884	21.513002364066196	31.96742842132913	27.396900446545835
42	23.850801155765694	22.353559232991856	30.575256107171	23.22038350407145
43	18.255844496979247	21.801943787759388	36.485421591804574	23.456790123456788
44	17.625426845285002	26.16233254531127	30.627790911478854	25.584449697924878
45	15.313895455739427	22.301024428684002	37.37851326503809	25.00656685053848
46	19.516679800367744	22.537431048069344	32.54531126871553	25.400577882847386
47	15.103756238508012	23.771998949303914	32.597846073023376	28.526398739164698
48	14.972419227738376	23.64066193853428	32.46650906225375	28.920409771473597
49	18.497109826589593	23.857067787703627	31.923279033105622	25.722543352601157
50	13.636363636363635	23.51550183920126	35.44403573305308	27.40409879138203
51	14.18812401471361	23.568050446663165	30.320546505517605	31.923279033105622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	1.0
20	2.0
21	2.5
22	3.0
23	2.0
24	1.0
25	2.0
26	7.5
27	12.0
28	17.0
29	22.0
30	34.5
31	47.0
32	65.0
33	83.0
34	122.5
35	162.0
36	199.0
37	236.0
38	283.5
39	331.0
40	365.5
41	400.0
42	414.0
43	428.0
44	411.5
45	395.0
46	391.5
47	388.0
48	362.5
49	337.0
50	279.5
51	222.0
52	242.5
53	263.0
54	206.0
55	149.0
56	121.0
57	93.0
58	77.0
59	61.0
60	52.0
61	43.0
62	38.5
63	34.0
64	30.0
65	26.0
66	24.0
67	22.0
68	17.5
69	13.0
70	12.0
71	11.0
72	9.5
73	8.0
74	5.5
75	2.5
76	2.0
77	3.0
78	4.0
79	3.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026267402153926978
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.026267402153926978
50	0.026267402153926978
51	0.026267402153926978
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3807.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.56080903598634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.06542056074767	94.66771736275282
2	0.4947773501924134	0.9456264775413712
3	0.10995052226498077	0.31520882584712373
4	0.027487630566245192	0.10506960861570791
5	0.08246289169873557	0.3940110323089046
6	0.0	0.0
7	0.027487630566245192	0.18387181507748881
8	0.0	0.0
9	0.0	0.0
>10	0.19241341396371633	3.38849487785658
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	27	0.7092198581560284	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	25	0.6566850538481744	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	21	0.5516154452324665	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC	19	0.4990806409246125	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC	14	0.36774363015497763	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	12	0.31520882584712373	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	11	0.28894142369319675	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC	7	0.18387181507748881	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	5	0.1313370107696349	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	5	0.1313370107696349	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	5	0.1313370107696349	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	4	0.10506960861570791	TruSeq Adapter, Index 12 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.026267402153926978
4	0.0	0.0	0.052534804307853955
5	0.0	0.0	0.052534804307853955
6	0.0	0.0	0.052534804307853955
7	0.0	0.0	0.052534804307853955
8	0.0	0.0	0.052534804307853955
9	0.0	0.0	0.052534804307853955
10	0.0	0.0	0.052534804307853955
11	0.0	0.0	0.052534804307853955
12	0.0	0.0	0.052534804307853955
13	0.0	0.0	0.052534804307853955
14	0.0	0.0	0.052534804307853955
15	0.0	0.0	0.052534804307853955
16	0.0	0.0	0.052534804307853955
17	0.0	0.0	0.052534804307853955
18	0.0	0.0	0.052534804307853955
19	0.0	0.0	0.052534804307853955
20	0.0	0.0	0.052534804307853955
21	0.0	0.0	0.07880220646178093
22	0.0	0.0	0.07880220646178093
23	0.0	0.0	0.1313370107696349
24	0.0	0.0	0.1313370107696349
25	0.0	0.0	0.1313370107696349
26	0.0	0.0	0.15760441292356187
27	0.0	0.0	0.21013921723141582
28	0.0	0.0	0.39401103230890466
29	0.0	0.0	0.5516154452324665
30	0.0	0.0	1.1820330969267139
31	0.0	0.0	2.6267402153926978
32	0.0	0.0	4.0189125295508275
33	0.0	0.0	5.962700288941424
34	0.0	0.0	7.64381402679275
35	0.0	0.0	9.141055949566589
36	0.0	0.0	10.900971893879696
37	0.0	0.0	13.422642500656686
38	0.0	0.0	16.33832413974258
39	0.0	0.0	19.175203572366694
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCTCC	50	0.0018784639	22.5	45
ACACATC	50	0.0018784639	22.5	42
CACATCT	55	0.0032700105	20.454546	43
ACATCTC	60	0.0054159146	18.749998	44
TACACAT	60	0.0054159146	18.749998	41
ATACACA	65	0.008602736	17.307692	40
>>END_MODULE