FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_73.3410000000536b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_73.3410000000536b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC181.7458777885548011No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC151.454898157129001No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC50.4849660523763337RNA PCR Primer, Index 23 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTA30.2909796314258002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19398642095053348No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT20.19398642095053348No Hit
TCACAAGGTAGTGCACAACCACCTGTAATGGGATCTGATGTCCTCTTCCTG20.19398642095053348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19398642095053348No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA20.19398642095053348No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA20.19398642095053348No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19398642095053348No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)