FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_72.3410000000535f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_72.3410000000535f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978037
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATAC12480.12760253446444256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG501.9815361E-631.49935537
CCCGTAA453.487151E-530.0039881
TTCGTCG300.00514740229.99938837
TACTTAC4350.029.99938631
GCGATTA1900.029.60465824
GTATCAA10400.028.561491
TTGCGGG803.3942342E-928.12442629
CGATTAG2100.027.85657325
GTACTTA4800.027.18694530
TGTAAGT10950.026.3048671
GGGGACG1750.025.7137635
AGTGTAC7300.025.58166927
TGGCGAT2150.025.11576522
GGCGATT2250.024.9994923
GTGTACT6000.024.37450228
GGTATCA3550.024.087711
GTAAGTA12550.023.8441332
CTACGAC1052.3646862E-923.57215318
CAGTGTA7850.023.50270526
GCCGGTT500.002263294522.5018412
Produced by FastQC (version 0.11.2)