FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_71.34100000005342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_71.34100000005342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109731
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC119821.0797211216051457No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT17540.1580563217572547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13600.041.85959645
TATGCCG16000.035.4368343
CGAGACT16200.035.27710723
ATGCCGT16250.034.89164444
GCCCACG16500.034.63726418
CCCACGA16600.034.4270619
ACGAGAC16750.034.2530822
CTCGTAT16800.033.61543339
CCACGAG17150.033.5853720
TCGTATG16950.033.5834340
TATCTCG16700.033.4125436
GAGACTC17050.033.254524
TCTCGTA17000.033.21995538
ATCTCGT17250.032.8689437
GACTCAA17400.032.19766226
CACGAGA17900.032.17815821
CGAGCCC17950.031.96461915
AGACTCA18100.031.822625
AGCCCAC18000.031.75082617
CCGAGCC18700.030.68261514
Produced by FastQC (version 0.11.2)