FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_70.34100000005335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_70.34100000005335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC240.8978675645342313RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.6359895248784138No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC160.5985783763561541RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC130.4863449307893753No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC130.4863449307893753No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC100.3741114852225963RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.2618780396558174No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC70.2618780396558174No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC60.22446689113355783No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC60.22446689113355783No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC40.14964459408903852No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC40.14964459408903852No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT30.11223344556677892No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC30.11223344556677892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC252.9346324E-545.00000417
GCCCACG252.9346324E-545.00000418
TGCCGTC252.9346324E-545.00000445
GAGCCCA252.9346324E-545.00000416
CCCACGA252.9346324E-545.00000419
TATCTCG205.8342406E-445.036
CGAGCCC205.8342406E-445.015
CCGAGCC205.8342406E-445.014
TCCGAGC205.8342406E-445.013
GTATGCC308.615986E-537.50000442
ATCTCGT308.615986E-537.50000437
CCACGAG308.615986E-537.50000420
TATGCCG308.615986E-537.50000443
CACGAGA308.615986E-537.50000421
ACATCTC308.615986E-537.5000048
TCTCGTA308.615986E-537.50000438
ACGAGAC308.615986E-537.50000422
ATGCCGT308.615986E-537.50000444
CGTATGC308.615986E-537.50000441
TCGTATG308.615986E-537.50000440