Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 18h_70.34100000005335.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2673 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 24 | 0.8978675645342313 | RNA PCR Primer, Index 45 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC | 17 | 0.6359895248784138 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC | 16 | 0.5985783763561541 | RNA PCR Primer, Index 13 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC | 13 | 0.4863449307893753 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC | 13 | 0.4863449307893753 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC | 10 | 0.3741114852225963 | RNA PCR Primer, Index 23 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC | 7 | 0.2618780396558174 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC | 7 | 0.2618780396558174 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC | 6 | 0.22446689113355783 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC | 6 | 0.22446689113355783 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC | 4 | 0.14964459408903852 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC | 4 | 0.14964459408903852 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT | 3 | 0.11223344556677892 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC | 3 | 0.11223344556677892 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCCCAC | 25 | 2.9346324E-5 | 45.000004 | 17 |
GCCCACG | 25 | 2.9346324E-5 | 45.000004 | 18 |
TGCCGTC | 25 | 2.9346324E-5 | 45.000004 | 45 |
GAGCCCA | 25 | 2.9346324E-5 | 45.000004 | 16 |
CCCACGA | 25 | 2.9346324E-5 | 45.000004 | 19 |
TATCTCG | 20 | 5.8342406E-4 | 45.0 | 36 |
CGAGCCC | 20 | 5.8342406E-4 | 45.0 | 15 |
CCGAGCC | 20 | 5.8342406E-4 | 45.0 | 14 |
TCCGAGC | 20 | 5.8342406E-4 | 45.0 | 13 |
GTATGCC | 30 | 8.615986E-5 | 37.500004 | 42 |
ATCTCGT | 30 | 8.615986E-5 | 37.500004 | 37 |
CCACGAG | 30 | 8.615986E-5 | 37.500004 | 20 |
TATGCCG | 30 | 8.615986E-5 | 37.500004 | 43 |
CACGAGA | 30 | 8.615986E-5 | 37.500004 | 21 |
ACATCTC | 30 | 8.615986E-5 | 37.500004 | 8 |
TCTCGTA | 30 | 8.615986E-5 | 37.500004 | 38 |
ACGAGAC | 30 | 8.615986E-5 | 37.500004 | 22 |
ATGCCGT | 30 | 8.615986E-5 | 37.500004 | 44 |
CGTATGC | 30 | 8.615986E-5 | 37.500004 | 41 |
TCGTATG | 30 | 8.615986E-5 | 37.500004 | 40 |