##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_7.34100000004f2f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6876 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.86212914485166 38.0 32.0 38.0 32.0 38.0 2 36.0065445026178 38.0 38.0 38.0 32.0 38.0 3 35.93615474112856 38.0 32.0 38.0 32.0 38.0 4 36.09453170447935 38.0 38.0 38.0 32.0 38.0 5 36.12056428155905 38.0 38.0 38.0 32.0 38.0 6 37.975130890052355 40.0 38.0 40.0 32.0 40.0 7 37.759744037230945 40.0 38.0 40.0 32.0 40.0 8 37.70055264688772 40.0 38.0 40.0 32.0 40.0 9 37.9000872600349 40.0 38.0 40.0 32.0 40.0 10 37.63976148923793 40.0 38.0 40.0 32.0 40.0 11 37.75770796974985 40.0 38.0 40.0 32.0 40.0 12 37.61242001163467 40.0 38.0 40.0 32.0 40.0 13 37.58435136707388 40.0 38.0 40.0 32.0 40.0 14 37.56937172774869 40.0 38.0 40.0 32.0 40.0 15 37.567626527050614 40.0 38.0 40.0 32.0 40.0 16 37.56384525887144 40.0 38.0 40.0 32.0 40.0 17 37.59176847004072 40.0 38.0 40.0 32.0 40.0 18 37.588568935427574 40.0 38.0 40.0 32.0 40.0 19 37.568062827225134 40.0 38.0 40.0 32.0 40.0 20 37.603403141361255 40.0 38.0 40.0 32.0 40.0 21 37.71247818499128 40.0 38.0 40.0 32.0 40.0 22 37.56893542757417 40.0 38.0 40.0 32.0 40.0 23 37.58391506689936 40.0 38.0 40.0 32.0 40.0 24 37.503199534613145 40.0 38.0 40.0 32.0 40.0 25 37.55497382198953 40.0 38.0 40.0 32.0 40.0 26 37.49258289703316 40.0 38.0 40.0 32.0 40.0 27 37.45491564863293 40.0 38.0 40.0 32.0 40.0 28 37.44866201279814 40.0 38.0 40.0 32.0 40.0 29 37.360383944153575 40.0 38.0 40.0 32.0 40.0 30 37.43324607329843 40.0 38.0 40.0 32.0 40.0 31 37.40183246073298 40.0 38.0 40.0 32.0 40.0 32 37.288539848749274 40.0 38.0 40.0 32.0 40.0 33 37.28417684700407 40.0 38.0 40.0 32.0 40.0 34 37.31966259453171 40.0 38.0 40.0 32.0 40.0 35 37.29610238510762 40.0 38.0 40.0 32.0 40.0 36 37.29755671902269 40.0 38.0 40.0 32.0 40.0 37 37.324316463059915 40.0 38.0 40.0 32.0 40.0 38 37.26512507271669 40.0 38.0 40.0 32.0 40.0 39 37.31239092495637 40.0 38.0 40.0 32.0 40.0 40 37.20098894706224 40.0 38.0 40.0 32.0 40.0 41 37.19982547993019 40.0 38.0 40.0 32.0 40.0 42 37.260325770796975 40.0 38.0 40.0 32.0 40.0 43 37.27981384525887 40.0 38.0 40.0 32.0 40.0 44 37.252326934264104 40.0 38.0 40.0 32.0 40.0 45 37.210732984293195 40.0 38.0 40.0 32.0 40.0 46 37.1000581733566 38.0 38.0 40.0 32.0 40.0 47 37.10703897614892 38.0 38.0 40.0 32.0 40.0 48 37.075916230366495 38.0 38.0 40.0 32.0 40.0 49 37.04522978475858 38.0 38.0 40.0 32.0 40.0 50 36.99171029668412 38.0 38.0 40.0 32.0 40.0 51 36.447207678883075 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.7698818542568517 2201 2 0.3015220023032512 2201 3 0.9215768606393553 2201 4 0.28033719752470176 2201 5 -3.4986536900599248 2201 6 -2.51863514090077 2201 7 -1.2540452602952783 2201 8 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37 -0.5852703719891252 1209 38 -0.7427016126234847 1209 39 -0.21540469079531732 1209 40 1.0050069721944723 1209 41 1.0345448388417111 1209 42 -0.5469631496975254 1209 43 2.155945790320793 1209 44 0.8151862894050552 1209 45 0.8203516535547735 1209 46 0.9523022550366349 1209 47 1.4765190972222157 1209 48 -0.6713684319153046 1209 49 1.5942533594877304 1209 50 0.3973501325063822 1209 51 3.276304554820186 1214 1 0.8455027611277615 1214 2 0.7169066176878616 1214 3 1.6600383991008982 1214 4 0.23418335137085222 1214 5 0.05519246378622711 1214 6 -0.018635140900769898 1214 7 0.7536470473970311 1214 8 0.5008067886974246 1214 9 -1.2289614031801506 1214 10 -0.9532769747613585 1214 11 -1.2502405146936368 1214 12 -1.3931134837384889 1214 13 -2.569069515553892 1214 14 -3.7159267815517936 1214 15 0.1592478701853679 1214 16 0.3986919330669281 1214 17 0.6998602525946467 1214 18 0.8633599473443283 1214 19 1.2339134389915785 1214 20 0.8234333374958211 1214 21 0.6849346833721981 1214 22 0.4120376151626033 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-5.778240899725269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 4.0 23 3.0 24 11.0 25 18.0 26 46.0 27 59.0 28 83.0 29 115.0 30 136.0 31 153.0 32 209.0 33 217.0 34 290.0 35 321.0 36 414.0 37 659.0 38 1057.0 39 3077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.23101542042479 18.73727087576375 11.448938027349433 31.58277567646203 2 17.030250145433392 19.284467713787084 38.089005235602095 25.59627690517743 3 19.63350785340314 22.614892379290286 30.948225712623618 26.803374054682955 4 15.532286212914483 20.24432809773124 32.24258289703316 31.980802792321118 5 16.463059918557303 31.70447934845841 34.889470622454915 16.942990110529376 6 32.06806282722513 36.940081442699245 15.415939499709133 15.575916230366493 7 27.66143106457243 27.89412449098313 20.433391506689937 24.01105293775451 8 25.0 36.57649796393252 19.473531122745783 18.9499709133217 9 23.778359511343805 15.6195462478185 19.63350785340314 40.968586387434556 10 17.64107038976149 29.217568353694006 27.66143106457243 25.479930191972077 11 34.3804537521815 22.13496218731821 25.479930191972077 18.004653868528216 12 21.78592204770215 22.96393251890634 27.705061082024436 27.545084351367073 13 27.952297847585804 19.342641070389764 27.545084351367073 25.15997673065736 14 22.22222222222222 20.069808027923212 24.14194299011053 33.56602675974404 15 25.45084351367074 25.24723676556137 20.53519488074462 28.766724840023265 16 28.15590459569517 26.41070389761489 21.52414194299011 23.909249563699824 17 23.007562536358346 28.708551483420592 25.13089005235602 23.15299592786504 18 27.123327515997676 25.029086678301336 24.40372309482257 23.44386271087842 19 24.927283304246657 24.171029668411865 23.502036067481093 27.399650959860384 20 23.56020942408377 24.461896451425247 23.9674229203025 28.01047120418848 21 23.036649214659686 24.60732984293194 23.007562536358346 29.348458406050028 22 24.43280977312391 28.635834787667246 23.25479930191972 23.676556137289122 23 23.211169284467715 24.345549738219894 23.909249563699824 28.534031413612563 24 27.44328097731239 24.40372309482257 23.531122745782433 24.621873182082606 25 24.505526468877253 27.923211169284468 23.5456660849331 24.02559627690518 26 28.1413612565445 25.30541012216405 23.312972658522398 23.24025596276905 27 23.10936591041303 28.490401396160557 24.098312972658523 24.301919720767888 28 24.200116346713205 23.72018615474113 23.269342641070388 28.81035485747528 29 23.72018615474113 27.908667830133798 24.665503199534612 23.705642815590462 30 22.658522396742292 27.923211169284468 24.70913321698662 24.70913321698662 31 24.970913321698664 24.73821989528796 26.17801047120419 24.112856311809193 32 25.88714368819081 24.665503199534612 26.27981384525887 23.16753926701571 33 24.1564863292612 23.763816172193135 24.214659685863875 27.86503781268179 34 26.77428737638162 22.876672484002327 24.898196625945317 25.45084351367074 35 24.374636416521234 22.84758580570099 28.257707969749855 24.520069808027923 36 23.82198952879581 22.658522396742292 27.821407795229785 25.698080279232112 37 22.876672484002327 26.090750436300176 25.625363583478766 25.407213496218734 38 22.440372309482257 21.39325189063409 29.75567190226876 26.41070389761489 39 21.81500872600349 20.520651541593953 28.068644560791157 29.595695171611403 40 22.96393251890634 20.578824898196626 31.355439208842352 25.101803374054683 41 21.97498545666085 20.666084933100638 28.01047120418848 29.348458406050028 42 25.82897033158813 20.607911576497965 28.868528214077955 24.694589877835952 43 21.39325189063409 21.53868528214078 31.180919139034323 25.88714368819081 44 19.299011052937754 25.683536940081442 29.84293193717277 25.174520069808025 45 19.96800465386853 22.469458987783593 31.646305991855726 25.916230366492147 46 25.13089005235602 21.320535194880744 27.835951134380455 25.71262361838278 47 19.546247818499126 22.673065735892962 28.679464805119252 29.101221640488657 48 19.415357766143106 22.367655613728914 27.64688772542176 30.570098894706227 49 22.41128563118092 22.469458987783593 28.039557882489817 27.07969749854567 50 18.833624200116347 22.52763234438627 31.515415939499707 27.123327515997676 51 18.07737056428156 22.80395578824898 27.981384525887144 31.137289121582317 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.5 14 1.0 15 2.0 16 3.0 17 5.5 18 8.0 19 6.0 20 4.0 21 9.0 22 14.0 23 20.0 24 26.0 25 29.0 26 49.5 27 67.0 28 83.0 29 99.0 30 145.0 31 191.0 32 228.0 33 265.0 34 300.0 35 335.0 36 355.0 37 375.0 38 384.0 39 393.0 40 405.5 41 418.0 42 374.5 43 331.0 44 339.5 45 348.0 46 336.5 47 325.0 48 331.5 49 338.0 50 430.5 51 523.0 52 406.5 53 290.0 54 276.5 55 263.0 56 276.0 57 289.0 58 293.0 59 297.0 60 291.0 61 285.0 62 262.5 63 240.0 64 243.0 65 246.0 66 224.5 67 203.0 68 194.5 69 186.0 70 160.0 71 134.0 72 127.5 73 121.0 74 103.0 75 68.0 76 51.0 77 43.5 78 36.0 79 33.0 80 30.0 81 19.0 82 8.0 83 7.5 84 7.0 85 5.0 86 3.0 87 2.5 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029086678301337987 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 6876.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.67713787085515 #Duplication Level Percentage of deduplicated Percentage of total 1 98.57142857142858 93.32460732984293 2 1.1367127496159755 2.152414194299011 3 0.04608294930875576 0.13089005235602094 4 0.030721966205837174 0.11634671320535195 5 0.06144393241167435 0.29086678301337987 6 0.04608294930875576 0.2617801047120419 7 0.0 0.0 8 0.030721966205837174 0.2326934264107039 9 0.0 0.0 >10 0.06144393241167435 0.8580570098894705 >50 0.0 0.0 >100 0.015360983102918587 2.632344386271088 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC 181 2.632344386271088 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT 18 0.2617801047120419 No Hit CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 15 0.2181500872600349 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 14 0.2036067481093659 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 12 0.17452006980802792 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 8 0.11634671320535195 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG 8 0.11634671320535195 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.014543339150668994 19 0.0 0.0 0.014543339150668994 20 0.0 0.0 0.014543339150668994 21 0.0 0.0 0.014543339150668994 22 0.0 0.0 0.014543339150668994 23 0.0 0.0 0.014543339150668994 24 0.0 0.0 0.014543339150668994 25 0.0 0.0 0.04363001745200698 26 0.0 0.0 0.07271669575334497 27 0.0 0.0 0.10180337405468295 28 0.0 0.0 0.2472367655613729 29 0.0 0.0 0.4363001745200698 30 0.0 0.0 0.6980802792321117 31 0.0 0.0 1.3816172193135543 32 0.0 0.0 2.283304246655032 33 0.0 0.0 3.2286212914485164 34 0.0 0.0 4.552065154159395 35 0.0 0.0 5.64281559045957 36 0.0 0.0 7.009889470622455 37 0.0 0.0 8.609656777196044 38 0.0 0.0 10.514834205933683 39 0.0 0.0 12.638161721931356 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 45 2.7830538E-10 45.000004 42 CAATGCG 30 1.8657538E-6 45.000004 28 ATCTCGT 45 2.7830538E-10 45.000004 37 CGTTATC 30 1.8657538E-6 45.000004 33 ATCTCCG 45 2.7830538E-10 45.000004 10 TGCCGTC 45 2.7830538E-10 45.000004 45 CCACGAG 45 2.7830538E-10 45.000004 20 TTATCTC 30 1.8657538E-6 45.000004 35 TATGCCG 45 2.7830538E-10 45.000004 43 CACGAGA 45 2.7830538E-10 45.000004 21 CCCACGA 45 2.7830538E-10 45.000004 19 GACAATG 30 1.8657538E-6 45.000004 26 ACGAGAC 45 2.7830538E-10 45.000004 22 ATGCCGT 45 2.7830538E-10 45.000004 44 CGTATGC 45 2.7830538E-10 45.000004 41 GCGTTAT 30 1.8657538E-6 45.000004 32 TCGTATG 45 2.7830538E-10 45.000004 40 TCTCCGA 45 2.7830538E-10 45.000004 11 TCCGAGC 45 2.7830538E-10 45.000004 13 GTTATCT 30 1.8657538E-6 45.000004 34 >>END_MODULE