FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_7.34100000004f2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_7.34100000004f2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6876
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC1812.632344386271088No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGT180.2617801047120419No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC150.2181500872600349RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC140.2036067481093659No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC120.17452006980802792No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC80.11634671320535195No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCG80.11634671320535195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC452.7830538E-1045.00000442
CAATGCG301.8657538E-645.00000428
ATCTCGT452.7830538E-1045.00000437
CGTTATC301.8657538E-645.00000433
ATCTCCG452.7830538E-1045.00000410
TGCCGTC452.7830538E-1045.00000445
CCACGAG452.7830538E-1045.00000420
TTATCTC301.8657538E-645.00000435
TATGCCG452.7830538E-1045.00000443
CACGAGA452.7830538E-1045.00000421
CCCACGA452.7830538E-1045.00000419
GACAATG301.8657538E-645.00000426
ACGAGAC452.7830538E-1045.00000422
ATGCCGT452.7830538E-1045.00000444
CGTATGC452.7830538E-1045.00000441
GCGTTAT301.8657538E-645.00000432
TCGTATG452.7830538E-1045.00000440
TCTCCGA452.7830538E-1045.00000411
TCCGAGC452.7830538E-1045.00000413
GTTATCT301.8657538E-645.00000434