H23NVBCXX l02n01 18h_69.34100000005328.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 18h_69.34100000005328.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5766
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	113	1.9597641345820327	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	106	1.8383628165105792	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	85	1.4741588622962192	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	85	1.4741588622962192	RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC	80	1.387443635102324	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	34	0.5896635449184877	RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	19	0.32951786333680194	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC	17	0.29483177245924386	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	14	0.2428026361429067	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT	11	0.19077349982656955	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	11	0.19077349982656955	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG	10	0.1734304543877905	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC	10	0.1734304543877905	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	9	0.15608740894901144	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	9	0.15608740894901144	RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC	9	0.15608740894901144	RNA PCR Primer, Index 45 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG	8	0.1387443635102324	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT	6	0.10405827263267431	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT	6	0.10405827263267431	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	6	0.10405827263267431	RNA PCR Primer, Index 27 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCTT	20	6.463368E-4	45.0	27
TGCCGTC	80	0.0	45.0	45
GTGTATC	25	3.426709E-5	45.0	33
GTATCTC	40	5.013135E-9	45.0	35
GATCGTG	20	6.463368E-4	45.0	29
TCGTGTA	20	6.463368E-4	45.0	31
AGACGTC	20	6.463368E-4	45.0	25
TATCTCG	45	2.6193447E-10	45.0	36
AGACGAT	20	6.463368E-4	45.0	25
GACGATC	20	6.463368E-4	45.0	26
CGTGTAT	20	6.463368E-4	45.0	32
ACGATCG	20	6.463368E-4	45.0	27
ATCGTGT	20	6.463368E-4	45.0	30
CGAGACG	40	5.013135E-9	45.0	23
TGTATCT	25	3.426709E-5	45.0	34
CGTCTTA	20	6.463368E-4	45.0	28
GAGACGA	20	6.463368E-4	45.0	24
CGATCGT	20	6.463368E-4	45.0	28
GTATGCC	85	0.0	42.352943	42
ATCTCGT	85	0.0	42.352943	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content