##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_69.34100000005328.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5766 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.22528616024974 38.0 32.0 38.0 27.0 38.0 2 34.57023933402706 38.0 32.0 38.0 27.0 38.0 3 34.560700659035724 38.0 32.0 38.0 32.0 38.0 4 34.94693028095734 38.0 32.0 38.0 32.0 38.0 5 35.066423864030526 38.0 32.0 38.0 32.0 38.0 6 36.43652445369407 38.0 38.0 40.0 32.0 40.0 7 36.13302115851543 38.0 38.0 40.0 32.0 40.0 8 36.15244536940687 38.0 38.0 40.0 32.0 40.0 9 36.29673950745751 38.0 38.0 40.0 32.0 40.0 10 35.93201526187999 38.0 38.0 40.0 27.0 40.0 11 36.08428720083246 38.0 38.0 40.0 32.0 40.0 12 35.84044398196323 38.0 38.0 40.0 27.0 40.0 13 35.93635102323968 38.0 38.0 40.0 27.0 40.0 14 35.75043357613597 38.0 38.0 40.0 27.0 40.0 15 35.89403399236906 38.0 38.0 40.0 27.0 40.0 16 35.759625390218524 38.0 38.0 40.0 27.0 40.0 17 35.66267776621575 38.0 32.0 40.0 27.0 40.0 18 35.68574401664932 38.0 32.0 40.0 27.0 40.0 19 35.763787721123826 38.0 38.0 40.0 27.0 40.0 20 35.70967741935484 38.0 38.0 40.0 27.0 40.0 21 35.74401664932362 38.0 38.0 40.0 27.0 40.0 22 35.78113076656261 38.0 38.0 40.0 27.0 40.0 23 35.783038501560874 38.0 38.0 40.0 27.0 40.0 24 35.581685744016646 38.0 32.0 40.0 27.0 40.0 25 35.50017343045439 38.0 32.0 40.0 27.0 40.0 26 35.35345126604232 38.0 32.0 40.0 27.0 40.0 27 35.38120013874436 38.0 32.0 40.0 27.0 40.0 28 35.344259451959765 38.0 32.0 40.0 27.0 40.0 29 35.38172043010753 38.0 32.0 40.0 27.0 40.0 30 35.4360041623309 38.0 32.0 40.0 27.0 40.0 31 35.38969823100936 38.0 38.0 40.0 27.0 40.0 32 35.17360388484218 38.0 32.0 40.0 27.0 40.0 33 35.21193201526188 38.0 32.0 40.0 27.0 40.0 34 35.420395421436005 38.0 32.0 40.0 27.0 40.0 35 35.34772806104752 38.0 32.0 40.0 27.0 40.0 36 35.306798473812 38.0 32.0 40.0 27.0 40.0 37 35.20083246618106 38.0 32.0 40.0 27.0 40.0 38 35.46531390912244 38.0 32.0 40.0 27.0 40.0 39 35.35778702740201 38.0 32.0 40.0 27.0 40.0 40 35.30939993062782 38.0 32.0 40.0 27.0 40.0 41 35.41103017689906 38.0 38.0 40.0 27.0 40.0 42 35.29084287200833 38.0 32.0 40.0 27.0 40.0 43 35.39420742282345 38.0 32.0 40.0 27.0 40.0 44 35.23222337842525 38.0 32.0 40.0 27.0 40.0 45 35.46132500867152 38.0 38.0 40.0 27.0 40.0 46 35.05757891085675 38.0 32.0 40.0 27.0 40.0 47 35.008498092265 38.0 32.0 40.0 27.0 40.0 48 35.16129032258065 38.0 32.0 40.0 27.0 40.0 49 34.926812348248355 38.0 32.0 40.0 27.0 40.0 50 34.960457856399586 38.0 32.0 40.0 27.0 40.0 51 34.10978147762747 38.0 32.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 2.361771908646908 2201 2 -0.47366526702463574 2201 3 -0.45294215333277776 2201 4 -0.849500716297598 2201 5 1.4267554060522798 2201 6 1.5188632114413423 2201 7 -1.2657434579309452 2201 8 0.37212678814240974 2201 9 0.7472253007409222 2201 10 2.26486681721056 2201 11 -3.1087763798701147 2201 12 2.008776163073044 2201 13 -4.8058164491758255 2201 14 2.434834132881001 2201 15 -1.3164333062770481 2201 16 -3.9216889880952372 2201 17 3.181438266594519 2201 18 0.4361104607198385 2201 19 0.9320819805194844 2201 20 -1.327573338120196 2201 21 1.001844466297598 2201 22 -0.9630735150266432 2201 23 0.5138706779331699 2201 24 -1.5839139159451676 2201 25 3.0400186618936615 2201 26 -0.7817225309412876 2201 27 0.48154493076367544 2201 28 1.0599363310300944 2201 29 2.251496637043523 2201 30 1.6178585303585322 2201 31 -1.7871631406787714 2201 32 -0.06448638167388054 2201 33 0.8246987387612421 2201 34 1.9990896690115392 2201 35 -2.3275253826035183 2201 36 1.9644716394716397 2201 37 2.3924397217366007 2201 38 -1.3233585858585997 2201 39 -2.495758148101892 2201 40 4.093059371184374 2201 41 2.6299652604340125 2201 42 -0.9133368367743344 2201 43 -3.5745209217865437 2201 44 -0.6478257419663578 2201 45 -4.769742843961584 2201 46 0.8204734068015256 2201 47 -1.5066153464590855 2201 48 -0.7888561091686057 2201 49 -2.2678124392968115 2201 50 -0.9408270982489881 2201 51 -6.433079594017098 1101 1 0.6316131784881804 1101 2 1.3993506059912377 1101 3 0.53118483079421 1101 4 0.07113420433732642 1101 5 1.141041120337995 1101 6 2.407752100330228 1101 7 1.9564787642912762 1101 8 1.3562537722693975 1101 9 1.4456379991536252 1101 10 -0.7986252462815031 1101 11 1.1928109217171823 1101 12 -1.2769381226412406 1101 13 1.5592629159035383 1101 14 1.7205484185952855 1101 15 0.8740428841991417 1101 16 -2.9058159722222214 1101 17 -0.6122125270562719 1101 18 3.3249993496087242 1101 19 1.7257327741702753 1101 20 2.3073472968004367 1101 21 2.4145428789960093 1101 22 0.49724394529081195 1101 23 0.8472040112665056 1101 24 2.844657512626263 1101 25 0.42097104284604114 1101 26 0.45637270715394607 1101 27 2.1323385815573275 1101 28 -0.86069858960483 1101 29 3.045147430694314 1101 30 0.205160117660121 1101 31 -0.5173218708374989 1101 32 1.7926564754689736 1101 33 0.3485082625707605 1101 34 2.586391256313128 1101 35 2.1169190618409246 1101 36 4.297804972804975 1101 37 1.6305349598318344 1101 38 0.9782287157286973 1101 39 -0.7497264020701522 1101 40 3.1724244505494497 1101 41 0.5823462128149615 1101 42 0.43586951243201355 1101 43 1.3778600305944053 1101 44 2.939475845335231 1101 45 2.5953365211177797 1101 46 2.137933724261842 1101 47 -0.4907423305860732 1101 48 2.6238423035298055 1101 49 1.2718701003857333 1101 50 3.21790306048117 1101 51 2.328825167887665 2202 1 3.476057622932622 2202 2 -0.3450938384532023 2202 3 2.1756292752386557 2202 4 0.8933564265595493 2202 5 1.341041120337998 2202 6 -0.7811367885586549 2202 7 2.334256542069056 2202 8 1.6006982167138375 2202 9 0.8900824435980681 2202 10 0.07915253149627688 2202 11 1.148366477272738 2202 12 -0.1769381226412392 2202 13 1.8370406936813168 2202 14 1.5205484185952827 2202 15 1.6407095508658074 2202 16 2.1497395833333357 2202 17 0.31000969516595234 2202 18 -0.16388953928016292 2202 19 1.2035105519480567 2202 20 1.0295695190226581 2202 21 -0.24101267655954928 2202 22 0.8083550564019291 2202 23 0.013870677933169873 2202 24 -0.7553424873737384 2202 25 -1.3456956238206246 2202 26 -0.01029395951271539 2202 27 -0.48988364066489254 2202 28 -0.3829208118270486 2202 29 0.12292520847208976 2202 30 -0.3392843267843233 2202 31 2.127122573606947 2202 32 2.5926564754689707 2202 33 0.38184159590409905 2202 34 0.6419468118686851 2202 35 2.772474617396483 2202 36 -2.3355283605283574 2202 37 2.0638682931651715 2202 38 1.8337842712842516 2202 39 2.361384709040962 2202 40 0.8502022283272268 2202 41 3.1156795461483 2202 42 2.358091734654238 2202 43 1.0111933639277382 2202 44 2.195031400890791 2202 45 3.3731142988955582 2202 46 3.8490448353729576 2202 47 1.9648132249694825 2202 48 -2.83171325202575 2202 49 3.360758989274622 2202 50 -0.32654138396327426 2202 51 3.2954918345543263 1102 1 0.6760576229326247 1102 2 1.6549061615467977 1102 3 2.27562927523865 1102 4 -0.30664357344045357 1102 5 -4.858958879662005 1102 6 1.5188632114413423 1102 7 0.7342565420690548 1102 8 1.4006982167138418 1102 9 -0.8099175564019347 1102 10 -1.7208474685037274 1102 11 0.648366477272738 1102 12 0.023061877358756533 1102 13 0.23704069368131542 1102 14 2.120548418595284 1102 15 1.7407095508658088 1102 16 2.449739583333333 1102 17 1.6100096951659495 1102 18 -0.5638895392801615 1102 19 1.6035105519480553 1102 20 2.1295695190226596 1102 21 -4.241012676559549 1102 22 -2.991644943598075 1102 23 -1.5861293220668315 1102 24 -1.0553424873737356 1102 25 -0.3456956238206246 1102 26 2.1897060404872803 1102 27 1.7101163593351032 1102 28 -3.182920811827053 1102 29 0.922925208472094 1102 30 0.8607156732156795 1102 31 0.1271225736069468 1102 32 -2.2073435245310264 1102 33 1.381841595904099 1102 34 -1.358053188131315 1102 35 -1.2275253826035168 1102 36 1.2644716394716369 1102 37 -0.6361317068348313 1102 38 1.2337842712842573 1102 39 -1.3386152909590407 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1.7783104482323182 1208 35 2.8542927992146616 1208 36 2.600835275835273 1208 37 3.6911410204378967 1208 38 1.442875180375161 1208 39 2.361384709040962 1208 40 2.4047476828726815 1208 41 3.097497727966477 1208 42 1.2035462801087817 1208 43 2.347557000291374 1208 44 -1.5049685991092119 1208 45 0.10038702616828488 1208 46 2.0672266535547763 1208 47 -3.3897322295759693 1208 48 0.6137412934287951 1208 49 -1.5665137379981076 1208 50 2.137094979673094 1208 51 2.2682191072816025 1209 1 3.476057622932622 1209 2 0.5549061615467963 1209 3 0.5756292752386543 1209 4 1.4933564265595507 1209 5 0.041041120337993675 1209 6 0.018863211441342287 1209 7 1.4342565420690576 1209 8 0.5006982167138432 1209 9 0.5900824435980709 1209 10 0.879152531496274 1209 11 2.348366477272741 1209 12 2.5230618773587565 1209 13 -3.4629593063186803 1209 14 -0.47945158140471733 1209 15 -0.7592904491341912 1209 16 3.0497395833333343 1209 17 2.210009695165951 1209 18 3.6361104607198413 1209 19 0.303510551948051 1209 20 1.6295695190226596 1209 21 2.6589873234404493 1209 22 0.10835505640192622 1209 23 2.2138706779331727 1209 24 1.344657512626263 1209 25 0.4543043761793797 1209 26 1.6897060404872803 1209 27 -2.889883640664891 1209 28 2.1170791881729514 1209 29 1.0229252084720954 1209 30 2.1607156732156767 1209 31 0.8271225736069425 1209 32 1.9926564754689764 1209 33 3.2818415959040976 1209 34 -0.5580531881313178 1209 35 2.0724746173964803 1209 36 0.06447163947164114 1209 37 -0.8361317068348271 1209 38 1.3337842712842516 1209 39 0.061384709040964935 1209 40 1.4502022283272282 1209 41 0.6156795461483 1209 42 -0.9419082653457664 1209 43 -1.6888066360722576 1209 44 1.9950314008907881 1209 45 2.9731142988955597 1209 46 -1.1509551646270424 1209 47 3.864813224969481 1209 48 3.66828674797425 1209 49 3.160758989274619 1209 50 2.97345861603673 1209 51 4.395491834554328 1214 1 -1.7017201548451553 1214 2 -1.3784271717865408 1214 3 -2.024370724761347 1214 4 -5.484421351218231 1214 5 -1.3034033241064478 1214 6 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0.9914250679875707 1214 43 0.4889711417055196 1214 44 -0.9494130435536547 1214 45 1.3731142988955582 1214 46 0.4712670575951776 1214 47 -0.3796312194749589 1214 48 -0.7094910298035302 1214 49 -0.6170187885031595 1214 50 -3.8932080506299407 1214 51 -2.4489526098901138 1215 1 0.2427242895992876 1215 2 2.954906161546795 1215 3 2.975629275238653 1215 4 1.2933564265595479 1215 5 -2.025625546328669 1215 6 2.5188632114413423 1215 7 2.7342565420690548 1215 8 2.134031550047176 1215 9 1.890082443598068 1215 10 -2.187514135170389 1215 11 1.7483664772727394 1215 12 2.7230618773587594 1215 13 2.337040693681317 1215 14 -1.1127849147380502 1215 15 2.040709550865806 1215 16 -0.5169270833333286 1215 17 -0.5566569715007148 1215 18 1.6027771273865028 1215 19 -1.9964894480519462 1215 20 2.1962361856893224 1215 21 2.858987323440452 1215 22 1.441688389735262 1215 23 1.3472040112665056 1215 24 -0.3220091540404013 1215 25 0.25430437617937685 1215 26 1.2897060404872818 1215 27 3.2434496926684417 1215 28 2.9170791881729485 1215 29 2.489591875138757 1215 30 -1.7392843267843219 1215 31 -0.23954409305972035 1215 32 -0.37401019119769074 1215 33 -2.6514917374292395 1215 34 3.141946811868685 1215 35 3.0058079507298174 1215 36 2.631138306138304 1215 37 -0.5361317068348299 1215 38 3.86711760461759 1215 39 3.694718042374298 1215 40 -0.21646443833943607 1215 41 4.249012879481633 1215 42 2.4914250679875707 1215 43 2.5445266972610767 1215 44 -0.00496859910921188 1215 45 3.0397809655622225 1215 46 0.4157115020396205 1215 47 -0.7685201083638518 1215 48 3.401620081307584 1215 49 4.494092322607955 1215 50 -0.39320805062993713 1215 51 0.4954918345543291 1212 1 0.7903433372183386 1212 2 1.5263347329753643 1212 3 1.4042007038100834 1212 4 -2.706643573440452 1212 5 -0.4303874510905743 1212 6 -4.195422502844373 1212 7 -1.2657434579309452 1212 8 -2.3421589261433056 1212 9 0.032939586455214 1212 10 0.8362953886391296 1212 11 -0.3944906655844065 1212 12 -2.7055095512126712 1212 13 -1.5201021634615373 1212 14 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51 -2.575936736874244 1213 1 -3.123942377067376 1213 2 0.35490616154679344 1213 3 0.5756292752386543 1213 4 2.093356426559545 1213 5 -1.658958879662002 1213 6 -4.681136788558657 1213 7 0.5342565420690519 1213 8 0.6006982167138375 1213 9 1.890082443598068 1213 10 2.379152531496274 1213 11 0.9483664772727423 1213 12 -1.876938122641242 1213 13 1.5370406936813197 1213 14 1.9205484185952884 1213 15 -5.859290449134193 1213 16 -1.3502604166666643 1213 17 0.8100096951659523 1213 18 -3.3638895392801587 1213 19 0.5035105519480538 1213 20 -0.47043048097734186 1213 21 -7.941012676559549 1213 22 -4.591644943598073 1213 23 -4.686129322066829 1213 24 -3.9553424873737377 1213 25 -1.8456956238206246 1213 26 1.589706040487279 1213 27 1.7101163593351032 1213 28 1.7170791881729457 1213 29 2.8229252084720926 1213 30 -2.239284326784322 1213 31 -1.6728774263930575 1213 32 3.192656475468972 1213 33 -6.518158404095903 1213 34 1.7419468118686865 1213 35 -4.127525382603519 1213 36 -1.2355283605283631 1213 37 -3.4361317068348285 1213 38 -1.6662157287157484 1213 39 0.9613847090409635 1213 40 -2.0497977716727718 1213 41 -7.8843204538517035 1213 42 -3.7419082653457636 1213 43 -5.288806636072259 1213 44 -4.304968599109213 1213 45 -3.426885701104439 1213 46 -0.9509551646270467 1213 47 -0.5351867750305175 1213 48 -6.131713252025751 1213 49 -4.839241010725381 1213 50 -2.6265413839632714 1213 51 -1.004508165445671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 3.0 22 6.0 23 17.0 24 29.0 25 54.0 26 86.0 27 95.0 28 151.0 29 183.0 30 228.0 31 271.0 32 276.0 33 342.0 34 387.0 35 462.0 36 536.0 37 720.0 38 947.0 39 971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.21769297484822 15.472679965307892 12.402428447528187 38.907198612315696 2 12.330905306971903 19.580298300381546 44.10336455081512 23.985431841831424 3 15.643426985778703 21.956295525494276 38.570933055844606 23.82934443288241 4 11.89732917100243 27.055150884495315 33.95768296912938 27.089836975372876 5 12.851196670135275 32.17134928893513 41.84876864377385 13.12868539715574 6 27.17655220256677 43.58307318765175 16.63198057578911 12.608394033992369 7 23.395768296912937 30.090183836281653 19.33749566423864 27.17655220256677 8 20.499479708636837 43.132154006243496 19.441553936871315 16.92681234824835 9 22.06035379812695 16.389177939646203 17.53381893860562 44.01664932362122 10 13.544918487686438 36.47242455775234 29.431148109608046 20.551508844953172 11 28.598681928546654 22.389871661463754 31.66840097121054 17.34304543877905 12 18.730489073881373 22.85813388831079 28.390565383281302 30.020811654526536 13 24.60978147762747 19.77107180020812 34.79014915019077 20.82899757197364 14 19.16406520985085 20.135275754422477 24.26292056885189 36.437738466874784 15 19.719042663891777 26.101283385362468 22.840790842872007 31.338883107873745 16 31.113423517169615 25.251474158862298 22.684703433922998 20.95039889004509 17 20.291363163371486 35.62261533125216 23.603884842178285 20.48213666319806 18 31.73777315296566 24.332292750607007 23.812001387443633 20.117932708983695 19 21.990981616371837 24.696496704821367 22.164412070759624 31.148109608047175 20 20.724939299340964 24.158862296219215 23.864030523759972 31.25216788067985 21 20.62088102670829 24.332292750607007 22.337842525147416 32.708983697537285 22 20.152618799861255 36.160249739854315 22.667360388484216 21.019771071800207 23 20.10058966354492 25.112729795352063 23.482483524106833 31.304197016996184 24 31.06139438085328 25.390218522372525 23.74262920568852 19.805757891085673 25 22.459243843218868 34.269857787027405 23.066250433576137 20.20464793617759 26 30.905306971904267 25.00867152271939 24.43635102323968 19.649670482136663 27 20.04856052722858 36.593825875823796 23.37842525147416 19.979188345473464 28 19.87513007284079 24.26292056885189 25.095386749913285 30.766562608394032 29 23.93340270551509 25.997225112729794 29.639264654873397 20.43010752688172 30 24.228234477974333 27.85293097467915 27.939646201873046 19.979188345473464 31 21.834894207422824 27.74887270204648 27.85293097467915 22.563302115851545 32 21.054457162677767 24.765868886576484 30.107526881720432 24.07214706902532 33 20.62088102670829 25.442247658688867 30.870620881026706 23.066250433576137 34 20.4474505723205 28.04370447450572 30.81859174471037 20.690253208463403 35 23.759972251127298 24.471037114117237 29.604578563995837 22.164412070759624 36 20.690253208463403 25.35553243149497 30.610475199445027 23.343739160596602 37 17.828650711064864 31.633714880332985 28.07839056538328 22.459243843218868 38 17.464446756850503 20.846340617412416 38.952480055497745 22.736732570239333 39 17.898022892819977 19.614984391259107 29.240374609781476 33.24661810613944 40 17.898022892819977 18.903919528269164 40.513354144987865 22.684703433922998 41 17.603191120360737 19.944502254595907 29.72597988206729 32.72632674297606 42 28.009018383628163 20.4474505723205 29.847381200138745 21.69614984391259 43 15.938258758237946 20.98508498092265 40.339923690600074 22.736732570239333 44 15.747485258411379 31.408255289628862 29.118973291710027 23.72528616024974 45 14.25598335067638 21.297259798820672 40.77349982656955 23.673257023933402 46 25.043357613596946 21.956295525494276 29.413805064169267 23.586541796739507 47 13.631633714880333 21.418661116892128 31.408255289628862 33.541449878598684 48 14.498785986819286 22.927506070065903 28.25182101977107 34.32188692334374 49 22.771418661116893 21.938952480055498 30.315643426985776 24.97398543184183 50 12.920568851890394 21.626777662157476 38.935137010058966 26.517516475893167 51 11.743278404163053 22.133564614050304 29.40156114483955 36.721595836947095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 2.0 18 3.0 19 2.0 20 1.0 21 2.5 22 4.0 23 4.5 24 5.0 25 7.0 26 11.5 27 14.0 28 29.5 29 45.0 30 62.5 31 80.0 32 121.5 33 163.0 34 207.0 35 251.0 36 283.0 37 315.0 38 361.5 39 408.0 40 457.5 41 507.0 42 502.5 43 498.0 44 505.5 45 513.0 46 518.5 47 524.0 48 623.0 49 722.0 50 672.0 51 622.0 52 518.5 53 415.0 54 332.5 55 250.0 56 208.0 57 166.0 58 136.5 59 107.0 60 87.0 61 67.0 62 49.5 63 32.0 64 25.0 65 18.0 66 12.5 67 7.0 68 8.0 69 9.0 70 6.0 71 3.0 72 3.5 73 4.0 74 3.0 75 1.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01734304543877905 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.01734304543877905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 5766.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.3281304197017 #Duplication Level Percentage of deduplicated Percentage of total 1 98.95935597879443 87.40894901144641 2 0.5105046141763204 0.9018383628165106 3 0.0785391714117416 0.20811654526534862 4 0.0196347928529354 0.0693721817551162 5 0.0392695857058708 0.1734304543877905 6 0.05890437855880621 0.31217481789802287 7 0.0 0.0 8 0.0196347928529354 0.1387443635102324 9 0.05890437855880621 0.46826222684703434 >10 0.1570783428234832 2.18522372528616 >50 0.05890437855880621 4.335761359694763 >100 0.0392695857058708 3.7981269510926117 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 113 1.9597641345820327 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 106 1.8383628165105792 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 85 1.4741588622962192 No Hit CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 85 1.4741588622962192 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 80 1.387443635102324 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 34 0.5896635449184877 RNA PCR Primer, Index 45 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT 19 0.32951786333680194 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 17 0.29483177245924386 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 14 0.2428026361429067 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT 11 0.19077349982656955 No Hit TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT 11 0.19077349982656955 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG 10 0.1734304543877905 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC 10 0.1734304543877905 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 9 0.15608740894901144 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 9 0.15608740894901144 RNA PCR Primer, Index 23 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC 9 0.15608740894901144 RNA PCR Primer, Index 45 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG 8 0.1387443635102324 No Hit TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT 6 0.10405827263267431 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT 6 0.10405827263267431 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 6 0.10405827263267431 RNA PCR Primer, Index 27 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.01734304543877905 2 0.0 0.0 0.01734304543877905 3 0.0 0.0 0.0346860908775581 4 0.0 0.0 0.0346860908775581 5 0.0 0.0 0.0346860908775581 6 0.0 0.0 0.0346860908775581 7 0.0 0.0 0.0346860908775581 8 0.0 0.0 0.0346860908775581 9 0.0 0.0 0.0346860908775581 10 0.0 0.0 0.05202913631633715 11 0.0 0.0 0.05202913631633715 12 0.0 0.0 0.05202913631633715 13 0.0 0.0 0.05202913631633715 14 0.0 0.0 0.05202913631633715 15 0.0 0.0 0.05202913631633715 16 0.0 0.0 0.05202913631633715 17 0.0 0.0 0.05202913631633715 18 0.0 0.0 0.05202913631633715 19 0.0 0.0 0.05202913631633715 20 0.0 0.0 0.05202913631633715 21 0.0 0.0 0.0693721817551162 22 0.0 0.0 0.08671522719389525 23 0.0 0.0 0.1387443635102324 24 0.0 0.0 0.15608740894901144 25 0.0 0.0 0.15608740894901144 26 0.0 0.0 0.22545959070412763 27 0.0 0.0 0.31217481789802287 28 0.0 0.0 0.4856052722858134 29 0.0 0.0 0.693721817551162 30 0.0 0.0 1.1272979535206382 31 0.0 0.0 2.3066250433576134 32 0.0 0.0 3.780783905653833 33 0.0 0.0 5.428373222337843 34 0.0 0.0 6.902532084634061 35 0.0 0.0 8.58480749219563 36 0.0 0.0 10.388484217828651 37 0.0 0.0 12.608394033992369 38 0.0 0.0 15.261879986125564 39 0.0 0.0 18.019424210891433 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCTT 20 6.463368E-4 45.0 27 TGCCGTC 80 0.0 45.0 45 GTGTATC 25 3.426709E-5 45.0 33 GTATCTC 40 5.013135E-9 45.0 35 GATCGTG 20 6.463368E-4 45.0 29 TCGTGTA 20 6.463368E-4 45.0 31 AGACGTC 20 6.463368E-4 45.0 25 TATCTCG 45 2.6193447E-10 45.0 36 AGACGAT 20 6.463368E-4 45.0 25 GACGATC 20 6.463368E-4 45.0 26 CGTGTAT 20 6.463368E-4 45.0 32 ACGATCG 20 6.463368E-4 45.0 27 ATCGTGT 20 6.463368E-4 45.0 30 CGAGACG 40 5.013135E-9 45.0 23 TGTATCT 25 3.426709E-5 45.0 34 CGTCTTA 20 6.463368E-4 45.0 28 GAGACGA 20 6.463368E-4 45.0 24 CGATCGT 20 6.463368E-4 45.0 28 GTATGCC 85 0.0 42.352943 42 ATCTCGT 85 0.0 42.352943 37 >>END_MODULE