FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_68.3410000000531b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_68.3410000000531b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763559
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC357564.682807746356208No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT101381.3277297497639344No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG24430.31994908055566107No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG207.0329563E-444.99849322
AGGCCCG207.0329563E-444.99849324
CGCACGT207.0329563E-444.99849323
GCCCGGT207.0329563E-444.99849326
TGCCGTC37450.044.2775645
CACGGTG250.002107012635.99879526
CCGGAGG250.002107012635.99879528
GGCCCGG250.002107012635.99879525
ATGCCGT49650.033.48830444
GTCAACG352.82005E-432.1417836
TATGCCG53250.031.26655843
CGTATGC53400.031.26299941
GCCCACG53350.031.25012618
CGAGACT53350.031.1657823
CCCACGA53500.031.16250819
ACGAGAC53600.031.14634722
TCTCGTA53250.031.13980138
CACGAGA53550.031.13341121
CTCGTAT53350.031.12360839
CCACGAG53600.031.1043720
Produced by FastQC (version 0.11.2)