##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_66.341000000052f0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2572 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.44245723172628 38.0 32.0 38.0 27.0 38.0 2 34.444012441679625 38.0 32.0 38.0 27.0 38.0 3 34.49183514774494 38.0 32.0 38.0 32.0 38.0 4 34.701010886469675 38.0 32.0 38.0 32.0 38.0 5 34.744167962674965 38.0 32.0 38.0 32.0 38.0 6 36.35614307931571 38.0 38.0 40.0 32.0 40.0 7 35.84409020217729 38.0 38.0 40.0 27.0 40.0 8 35.75466562986003 38.0 38.0 40.0 27.0 40.0 9 35.90357698289269 38.0 38.0 40.0 27.0 40.0 10 35.770217729393465 38.0 38.0 40.0 27.0 40.0 11 35.86819595645412 38.0 38.0 40.0 27.0 40.0 12 35.71772939346812 38.0 38.0 40.0 27.0 40.0 13 36.01088646967341 38.0 38.0 40.0 32.0 40.0 14 35.679626749611195 38.0 38.0 40.0 27.0 40.0 15 35.56765163297045 38.0 32.0 40.0 27.0 40.0 16 35.40357698289269 38.0 32.0 40.0 27.0 40.0 17 35.65746500777605 38.0 38.0 40.0 27.0 40.0 18 35.505832037325035 38.0 32.0 40.0 27.0 40.0 19 35.83398133748056 38.0 38.0 40.0 27.0 40.0 20 35.70256609642302 38.0 38.0 40.0 27.0 40.0 21 35.5898133748056 38.0 38.0 40.0 27.0 40.0 22 35.704510108864696 38.0 38.0 40.0 27.0 40.0 23 35.639580093312595 38.0 32.0 40.0 27.0 40.0 24 35.63258164852255 38.0 38.0 40.0 27.0 40.0 25 35.63724727838258 38.0 32.0 40.0 27.0 40.0 26 35.31881804043546 38.0 32.0 40.0 27.0 40.0 27 35.3549766718507 38.0 32.0 40.0 27.0 40.0 28 35.21772939346812 38.0 32.0 40.0 27.0 40.0 29 35.32348367029549 38.0 32.0 40.0 27.0 40.0 30 35.233281493001556 38.0 32.0 40.0 27.0 40.0 31 35.295489891135304 38.0 32.0 40.0 27.0 40.0 32 35.06765163297045 38.0 32.0 40.0 27.0 40.0 33 34.9957231726283 38.0 32.0 40.0 27.0 40.0 34 35.1049766718507 38.0 32.0 38.0 27.0 40.0 35 35.24339035769829 38.0 32.0 38.0 27.0 40.0 36 35.237947122861584 38.0 32.0 40.0 27.0 40.0 37 34.827760497667185 38.0 32.0 38.0 27.0 40.0 38 35.076982892690516 38.0 32.0 38.0 27.0 40.0 39 35.14191290824261 38.0 32.0 40.0 27.0 40.0 40 35.24688958009331 38.0 32.0 40.0 27.0 40.0 41 35.31842923794712 38.0 32.0 40.0 27.0 40.0 42 35.18234836702955 38.0 32.0 40.0 27.0 40.0 43 35.27216174183515 38.0 32.0 40.0 27.0 40.0 44 35.008942457231726 38.0 32.0 40.0 27.0 40.0 45 34.93895800933126 38.0 32.0 38.0 27.0 40.0 46 34.76244167962675 38.0 32.0 38.0 27.0 40.0 47 34.766718506998444 38.0 32.0 38.0 27.0 40.0 48 34.94906687402799 38.0 32.0 40.0 27.0 40.0 49 34.92262830482115 38.0 32.0 40.0 27.0 40.0 50 34.903965785381025 38.0 32.0 38.0 27.0 40.0 51 34.03615863141524 38.0 32.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 -2.485825892857143 2201 2 1.814471726190476 2201 3 -13.367150297619045 2201 4 -4.228831845238094 2201 5 -0.6147693452380949 2201 6 -1.362797619047619 2201 7 3.03396577380952 2201 8 -5.290215773809525 2201 9 -3.026599702380956 2201 10 -14.005245535714291 2201 11 -3.0613467261904717 2201 12 -6.146986607142846 2201 13 -1.367224702380959 2201 14 -0.9155877976190467 2201 15 -7.879910714285717 2201 16 3.032254464285714 2201 17 -1.2918154761904717 2201 18 -0.9848958333333329 2201 19 -2.332775297619051 2201 20 2.178757440476197 2201 21 1.0973214285714263 2201 22 0.908444940476187 2201 23 -6.033296130952376 2201 24 -6.019494047619045 2201 25 -6.275930059523805 2201 26 1.057663690476197 2201 27 0.9427827380952465 2201 28 -6.7651413690476225 2201 29 -7.428497023809523 2201 30 -9.153794642857143 2201 31 -6.881696428571434 2201 32 -0.6551711309523824 2201 33 -0.39215029761903963 2201 34 3.056101190476191 2201 35 -4.881733630952379 2201 36 -0.683370535714289 2201 37 -1.328869047619044 2201 38 -8.732440476190483 2201 39 -2.9136904761904745 2201 40 -0.6925595238095212 2201 41 -0.6838541666666629 2201 42 -4.361197916666665 2201 43 -2.056101190476191 2201 44 -7.053236607142846 2201 45 -0.08690476190475493 2201 46 -2.360788690476191 2201 47 -7.198251488095234 2201 48 -1.0452752976190425 2201 49 -6.441815476190467 2201 50 -6.827492559523801 2201 51 2.602083333333333 1101 1 3.1808407738095212 1101 2 3.814471726190476 1101 3 -0.7004836309523768 1101 4 2.104501488095238 1101 5 -0.9481026785714306 1101 6 1.637202380952381 1101 7 -5.966034226190477 1101 8 -0.6235491071428569 1101 9 -1.026599702380956 1101 10 -5.005245535714291 1101 11 -2.3946800595238074 1101 12 2.186346726190486 1101 13 -1.7005580357142946 1101 14 -5.582254464285715 1101 15 -1.2132440476190496 1101 16 -2.6344122023809504 1101 17 -1.958482142857136 1101 18 3.015104166666667 1101 19 1.667224702380949 1101 20 -5.487909226190471 1101 21 -5.236011904761906 1101 22 0.908444940476187 1101 23 -5.366629464285712 1101 24 -6.019494047619045 1101 25 -0.9425967261904731 1101 26 2.3909970238095326 1101 27 0.2761160714285822 1101 28 -2.0984747023809547 1101 29 0.9048363095238088 1101 30 -4.820461309523811 1101 31 -3.548363095238102 1101 32 3.011495535714282 1101 33 -13.392150297619043 1101 34 -0.6105654761904731 1101 35 -7.548400297619043 1101 36 -9.016703869047625 1101 37 -7.995535714285712 1101 38 -8.065773809523815 1101 39 -8.580357142857142 1101 40 -2.3592261904761855 1101 41 2.9828125000000014 1101 42 -0.36119791666666146 1101 43 -2.7227678571428555 1101 44 0.28009672619048587 1101 45 -4.0869047619047585 1101 46 -2.360788690476191 1101 47 -5.198251488095234 1101 48 -1.0452752976190425 1101 49 -0.7751488095237988 1101 50 -4.160825892857137 1101 51 -3.7312499999999993 2202 1 1.6808407738095212 2202 2 -5.435528273809524 2202 3 -2.4504836309523768 2202 4 -0.39549851190476204 2202 5 -4.698102678571431 2202 6 -4.112797619047619 2202 7 -1.3827008928571445 2202 8 -1.873549107142857 2202 9 -0.5265997023809561 2202 10 3.1614211309523768 2202 11 1.6053199404761926 2202 12 2.186346726190486 2202 13 1.2994419642857054 2202 14 1.7510788690476176 2202 15 -0.04657738095238528 2202 16 1.5322544642857139 2202 17 2.541517857142864 2202 18 0.5151041666666671 2202 19 2.167224702380949 2202 20 2.678757440476197 2202 21 2.930654761904762 2202 22 3.908444940476187 2202 23 -0.4499627976190439 2202 24 -3.9361607142857125 2202 25 0.5574032738095269 2202 26 2.3909970238095326 2202 27 1.2761160714285822 2202 28 -4.348474702380955 2202 29 0.4048363095238088 2202 30 -0.9037946428571431 2202 31 -5.548363095238102 2202 32 -4.738504464285718 2202 33 -10.725483630952375 2202 34 -3.193898809523809 2202 35 0.03493303571428896 2202 36 -2.7667038690476247 2202 37 -6.16220238095238 2202 38 -3.482440476190483 2202 39 -0.4970238095238102 2202 40 2.8074404761904788 2202 41 -4.267187499999999 2202 42 -0.9445312499999972 2202 43 -0.05610119047619122 2202 44 -2.969903273809514 2202 45 -2.1702380952380906 2202 46 -5.194122023809527 2202 47 2.1350818452380977 2202 48 2.121391369047622 2202 49 0.6415178571428655 2202 50 -2.244159226190469 2202 51 -3.147916666666667 1102 1 -1.6763020833333329 1102 2 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1.7800967261904859 1209 45 2.0797619047619094 1209 46 -2.444122023809527 1209 47 0.8850818452380977 1209 48 1.6213913690476218 1209 49 2.3915178571428655 1209 50 4.505840773809531 1209 51 3.602083333333333 1214 1 -9.152492559523811 1214 2 -1.8521949404761884 1214 3 3.2995163690476232 1214 4 2.104501488095238 1214 5 1.0518973214285694 1214 6 1.637202380952381 1214 7 0.3672991071428555 1214 8 -3.2902157738095212 1214 9 -0.026599702380956103 1214 10 -5.005245535714291 1214 11 2.6053199404761926 1214 12 2.85301339285715 1214 13 2.2994419642857054 1214 14 -7.582254464285715 1214 15 -1.2132440476190496 1214 16 -4.301078869047618 1214 17 2.041517857142864 1214 18 -5.318229166666665 1214 19 0.3338913690476133 1214 20 0.8454241071428612 1214 21 3.0973214285714263 1214 22 -4.758221726190481 1214 23 -7.366629464285712 1214 24 -1.3528273809523768 1214 25 -1.2759300595238088 1214 26 3.057663690476197 1214 27 2.276116071428582 1214 28 -9.098474702380955 1214 29 -7.428497023809523 1214 30 0.8462053571428569 1214 31 -0.548363095238102 1214 32 3.011495535714282 1214 33 -7.0588169642857075 1214 34 -4.610565476190477 1214 35 1.4515997023809533 1214 36 -0.683370535714289 1214 37 0.3377976190476204 1214 38 -13.39910714285715 1214 39 -12.913690476190478 1214 40 -4.359226190476189 1214 41 0.9828125000000014 1214 42 3.305468750000003 1214 43 2.943898809523809 1214 44 3.94676339285715 1214 45 4.246428571428574 1214 46 3.9725446428571374 1214 47 1.1350818452380977 1214 48 0.6213913690476218 1214 49 1.5581845238095298 1214 50 1.5058407738095312 1214 51 2.602083333333333 1215 1 1.9808407738095184 1215 2 1.4144717261904773 1215 3 2.0995163690476204 1215 4 0.5045014880952365 1215 5 -0.548102678571432 1215 6 -4.562797619047618 1215 7 1.3672991071428555 1215 8 -1.0235491071428555 1215 9 -1.4265997023809547 1215 10 -2.3385788690476232 1215 11 -0.39468005952380736 1215 12 1.3863467261904887 1215 13 -1.9005580357142975 1215 14 0.15107886904761614 1215 15 2.8534226190476133 1215 16 1.6322544642857153 1215 17 2.4415178571428626 1215 18 1.21510416666667 1215 19 1.2672247023809504 1215 20 2.5787574404761955 1215 21 2.8306547619047606 1215 22 3.708444940476184 1215 23 0.9000372023809575 1215 24 -0.6861607142857125 1215 25 -3.142596726190476 1215 26 -0.20900297619046881 1215 27 1.8761160714285836 1215 28 -1.698474702380956 1215 29 1.3048363095238074 1215 30 -3.3537946428571423 1215 31 -0.7483630952381048 1215 32 3.4114955357142804 1215 33 -2.5254836309523725 1215 34 0.8561011904761884 1215 35 -0.4150669642857139 1215 36 1.7832961309523725 1215 37 0.3377976190476204 1215 38 -4.732440476190483 1215 39 -2.647023809523809 1215 40 2.5074404761904816 1215 41 -3.6171875 1215 42 -0.89453125 1215 43 -3.2561011904761905 1215 44 -1.7199032738095141 1215 45 -5.62023809523809 1215 46 -3.0941220238095255 1215 47 -0.4649181547619037 1215 48 -1.9786086309523796 1215 49 3.6915178571428626 1215 50 -1.0941592261904702 1215 51 0.0020833333333314386 1212 1 0.43084077380952124 1212 2 3.814471726190476 1212 3 0.29951636904762324 1212 4 -2.145498511904762 1212 5 3.0518973214285694 1212 6 3.137202380952381 1212 7 -4.8827008928571445 1212 8 -2.873549107142857 1212 9 1.973400297619044 1212 10 -3.5885788690476232 1212 11 -6.394680059523807 1212 12 0.6863467261904859 1212 13 -5.450558035714295 1212 14 -9.248921130952382 1212 15 2.4534226190476147 1212 16 -3.217745535714286 1212 17 -0.20848214285713595 1212 18 -2.234895833333333 1212 19 2.667224702380949 1212 20 1.178757440476197 1212 21 2.930654761904762 1212 22 1.408444940476187 1212 23 2.300037202380956 1212 24 -0.6861607142857125 1212 25 -3.692596726190473 1212 26 -5.359002976190467 1212 27 0.7761160714285822 1212 28 1.9015252976190453 1212 29 -3.345163690476191 1212 30 -3.403794642857143 1212 31 -5.048363095238102 1212 32 -6.238504464285718 1212 33 -10.725483630952375 1212 34 -4.693898809523809 1212 35 -6.215066964285711 1212 36 -3.2667038690476247 1212 37 -0.4122023809523796 1212 38 -0.482440476190483 1212 39 1.7529761904761898 1212 40 -8.692559523809521 1212 41 -5.017187499999999 1212 42 -1.6945312499999972 1212 43 -4.806101190476191 1212 44 3.280096726190486 1212 45 -2.6702380952380906 1212 46 3.805877976190473 1212 47 2.1350818452380977 1212 48 2.621391369047622 1212 49 -1.1084821428571345 1212 50 -2.744159226190469 1212 51 0.8520833333333329 1213 1 -2.3191592261904788 1213 2 -11.685528273809524 1213 3 0.29951636904762324 1213 4 -1.895498511904762 1213 5 0.0518973214285694 1213 6 1.637202380952381 1213 7 -10.132700892857144 1213 8 -3.123549107142857 1213 9 1.973400297619044 1213 10 2.6614211309523768 1213 11 -8.894680059523807 1213 12 3.186346726190486 1213 13 -3.2005580357142946 1213 14 -2.7489211309523824 1213 15 -0.5465773809523853 1213 16 -12.467745535714286 1213 17 -3.958482142857136 1213 18 -5.484895833333333 1213 19 -1.332775297619051 1213 20 -0.8212425595238031 1213 21 2.430654761904762 1213 22 -15.091555059523813 1213 23 -10.199962797619044 1213 24 2.3138392857142875 1213 25 -10.442596726190473 1213 26 -0.6090029761904674 1213 27 -0.7238839285714178 1213 28 -0.09847470238095468 1213 29 -0.09516369047619122 1213 30 -12.653794642857143 1213 31 3.451636904761898 1213 32 -2.988504464285718 1213 33 3.2745163690476247 1213 34 0.05610119047619122 1213 35 -5.715066964285711 1213 36 -9.516703869047625 1213 37 -0.6622023809523796 1213 38 -9.232440476190483 1213 39 -2.74702380952381 1213 40 -2.1925595238095212 1213 41 -0.01718749999999858 1213 42 0.30546875000000284 1213 43 3.943898809523809 1213 44 0.28009672619048587 1213 45 0.5797619047619094 1213 46 -5.194122023809527 1213 47 3.1350818452380977 1213 48 2.621391369047622 1213 49 -9.608482142857135 1213 50 -8.994159226190469 1213 51 -7.897916666666667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 5.0 24 12.0 25 26.0 26 42.0 27 61.0 28 66.0 29 96.0 30 104.0 31 120.0 32 117.0 33 150.0 34 184.0 35 207.0 36 253.0 37 314.0 38 430.0 39 381.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.5875486381323 15.019455252918288 11.98443579766537 35.40856031128405 2 13.569206842923794 18.895800933125972 42.61275272161742 24.922239502332815 3 16.56298600311042 22.97822706065319 32.46500777604977 27.993779160186627 4 12.713841368584758 21.65629860031104 34.68118195956454 30.94867807153966 5 12.908242612752721 33.631415241057546 37.71384136858476 15.746500777604977 6 29.432348367029547 39.42457231726283 15.78538102643857 15.35769828926905 7 25.34992223950233 30.054432348367026 20.762052877138416 23.833592534992224 8 22.900466562986 39.15241057542768 18.312597200622083 19.63452566096423 9 24.805598755832037 16.018662519440124 17.65163297045101 41.52410575427683 10 14.463452566096425 32.8149300155521 29.665629860031107 23.055987558320375 11 31.337480559875587 23.989113530326595 26.98289269051322 17.690513219284604 12 21.18973561430793 24.57231726283048 27.33281493001555 26.905132192846033 13 25.738724727838257 21.15085536547434 29.704510108864696 23.405909797822705 14 20.606531881804045 21.30637636080871 23.950233281493002 34.13685847589424 15 23.367029548989112 27.488335925349922 21.61741835147745 27.527216174183515 16 27.91601866251944 26.67185069984448 25.0 20.412130637636082 17 21.77293934681182 31.726283048211506 23.678071539657854 22.82270606531882 18 28.6547433903577 25.738724727838257 24.805598755832037 20.80093312597201 19 23.055987558320375 25.194401244167963 26.39968895800933 25.34992223950233 20 23.250388802488338 24.68895800933126 23.63919129082426 28.421461897356142 21 22.900466562986 24.883359253499222 23.950233281493002 28.265940902021775 22 22.045101088646966 31.765163297045103 23.75583203732504 22.433903576982893 23 22.12286158631415 25.194401244167963 24.027993779160187 28.6547433903577 24 28.96578538102644 26.360808709175735 24.611197511664074 20.06220839813375 25 21.967340590979784 31.104199066874028 25.505443234836704 21.423017107309487 26 26.905132192846033 26.39968895800933 25.27216174183515 21.423017107309487 27 20.762052877138416 32.03732503888025 24.144634525660962 23.055987558320375 28 22.08398133748056 23.60031104199067 26.5552099533437 27.76049766718507 29 24.22239502332815 25.505443234836704 28.032659409020216 22.23950233281493 30 23.63919129082426 25.116640746500778 27.29393468118196 23.950233281493002 31 25.116640746500778 26.010886469673405 27.682737169517885 21.18973561430793 32 23.79471228615863 27.29393468118196 24.261275272161743 24.65007776049767 33 21.811819595645414 26.944012441679625 28.69362363919129 22.55054432348367 34 23.75583203732504 23.48367029548989 28.615863141524105 24.144634525660962 35 23.367029548989112 22.356143079315707 29.743390357698292 24.53343701399689 36 23.989113530326595 24.65007776049767 27.060653188180407 24.300155520995332 37 20.412130637636082 27.33281493001555 28.771384136858476 23.48367029548989 38 20.37325038880249 21.073094867807153 34.175738724727836 24.377916018662518 39 21.967340590979784 21.77293934681182 28.1104199066874 28.149300155520997 40 19.12908242612753 21.578538102643858 35.14774494556765 24.144634525660962 41 21.539657853810265 20.72317262830482 29.54898911353033 28.18818040435459 42 24.766718506998444 21.30637636080871 31.765163297045103 22.161741835147744 43 18.506998444790046 21.578538102643858 34.68118195956454 25.233281493001552 44 16.56298600311042 27.954898911353034 30.94867807153966 24.53343701399689 45 16.524105754276828 22.939346811819597 36.58631415241057 23.950233281493002 46 22.66718506998445 21.811819595645414 31.337480559875587 24.183514774494558 47 16.018662519440124 23.211508553654745 30.909797822706064 29.86003110419907 48 15.085536547433904 24.027993779160187 30.404354587869364 30.48211508553655 49 21.111975116640746 23.4447900466563 28.92690513219285 26.51632970451011 50 14.580093312597201 21.967340590979784 36.197511664074646 27.25505443234837 51 13.80248833592535 23.561430793157076 28.032659409020216 34.603421461897355 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 1.5 18 1.0 19 1.0 20 1.0 21 0.5 22 0.0 23 1.5 24 3.0 25 2.0 26 2.5 27 4.0 28 8.0 29 12.0 30 24.0 31 36.0 32 41.0 33 46.0 34 70.0 35 94.0 36 112.0 37 130.0 38 149.5 39 169.0 40 202.0 41 235.0 42 236.5 43 238.0 44 232.5 45 227.0 46 220.5 47 214.0 48 223.5 49 233.0 50 234.0 51 235.0 52 226.0 53 217.0 54 186.0 55 155.0 56 126.5 57 98.0 58 85.5 59 73.0 60 64.0 61 55.0 62 41.0 63 27.0 64 25.5 65 24.0 66 19.5 67 15.0 68 14.5 69 14.0 70 9.5 71 5.0 72 4.5 73 4.0 74 3.0 75 1.0 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07776049766718507 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.67340590979782 #Duplication Level Percentage of deduplicated Percentage of total 1 99.17864476386036 93.89580093312597 2 0.2874743326488706 0.5443234836702955 3 0.08213552361396304 0.23328149300155523 4 0.08213552361396304 0.3110419906687403 5 0.04106776180698152 0.19440124416796267 6 0.0 0.0 7 0.0 0.0 8 0.08213552361396304 0.6220839813374806 9 0.0 0.0 >10 0.24640657084188913 4.199066874027993 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 40 1.5552099533437014 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 17 0.6609642301710731 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 16 0.6220839813374806 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 14 0.5443234836702955 No Hit CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC 11 0.4276827371695179 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 10 0.38880248833592534 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC 8 0.3110419906687403 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC 8 0.3110419906687403 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 5 0.19440124416796267 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 4 0.15552099533437014 No Hit TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT 4 0.15552099533437014 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 3 0.11664074650077762 No Hit CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC 3 0.11664074650077762 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.038880248833592534 7 0.0 0.0 0.038880248833592534 8 0.0 0.0 0.038880248833592534 9 0.0 0.0 0.038880248833592534 10 0.0 0.0 0.038880248833592534 11 0.0 0.0 0.038880248833592534 12 0.0 0.0 0.038880248833592534 13 0.0 0.0 0.038880248833592534 14 0.0 0.0 0.038880248833592534 15 0.0 0.0 0.038880248833592534 16 0.0 0.0 0.038880248833592534 17 0.0 0.0 0.038880248833592534 18 0.0 0.0 0.038880248833592534 19 0.0 0.0 0.038880248833592534 20 0.0 0.0 0.038880248833592534 21 0.0 0.0 0.038880248833592534 22 0.0 0.0 0.038880248833592534 23 0.0 0.0 0.07776049766718507 24 0.0 0.0 0.1166407465007776 25 0.0 0.0 0.1166407465007776 26 0.0 0.0 0.15552099533437014 27 0.0 0.0 0.15552099533437014 28 0.0 0.0 0.3110419906687403 29 0.0 0.0 0.4665629860031104 30 0.0 0.0 1.088646967340591 31 0.0 0.0 2.2939346811819594 32 0.0 0.0 3.576982892690513 33 0.0 0.0 4.743390357698289 34 0.0 0.0 6.531881804043546 35 0.0 0.0 7.5816485225505446 36 0.0 0.0 9.409020217729394 37 0.0 0.0 11.625194401244167 38 0.0 0.0 14.307931570762053 39 0.0 0.0 17.146189735614307 >>END_MODULE >>Kmer Content pass >>END_MODULE