FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_64.341000000052d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_64.341000000052d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931839
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATAC22990.2467164392132117No Hit
TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATACAC13580.145733329470005No Hit
GTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATACACATC11060.11869003121783912No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA10190.10935365444030568No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG9760.10473912338934087No Hit
TGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGCGTCAGATC9330.10012459233837605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG356.2461477E-638.57221227
GACCGTA301.13962014E-437.5007611
TATGCCG1500.034.49884843
GCGCTAG401.5600346E-533.75068326
CGTATGC1550.033.38598341
TGCCGTC1600.032.34440645
ATGCCGT1650.031.3625944
TACTTAC4700.030.15856631
TGTAAGT22350.029.0016571
CTCGTAT1800.028.7490439
TCTCGTA1800.028.7490438
CACGCGG1600.026.71929217
GTACTTA5450.026.00970330
GTATCAA41800.026.006311
ACGCGGG1650.025.90961618
AATCTCG1700.025.1462236
GTAAGTA26100.025.085372
ACCGCGA450.001226619124.99916536
TACCGCG450.001226619124.99916535
CGGGGTG7650.024.70638317
Produced by FastQC (version 0.11.2)