FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_63.341000000052c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_63.341000000052c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446234
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC93020.6431877552318643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11350.041.23376545
TATGCCG12750.036.52966343
GCCCACG13150.036.4438618
CCCACGA13300.036.03283719
ATGCCGT13250.035.32144
CCACGAG13650.035.2737520
ACGAGAC13850.034.92683422
AATCTCG13050.034.82662236
CGAGCCC13950.034.51756315
CGAGACC13400.033.91697323
CTCGTAT13900.033.8339
CACGAGA14350.033.70986621
TCGTATG14400.032.81159240
TCTCGTA14400.032.65534638
CCGAGCC14750.032.64542414
GACCTGA14350.031.51589826
AGCCCAC15350.031.36721217
CGTATGC15150.031.03981841
ATCTCGT15500.030.48302737
ACCTGAT14900.030.3525627
Produced by FastQC (version 0.11.2)