FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_62.341000000052bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_62.341000000052bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507174
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC133690.8870243249949906No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT29790.1976546835335535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15650.043.2742545
TATGCCG19800.034.54504843
CCCACGA20300.034.0266919
CCACGAG20450.033.77710720
CGAGACC20100.033.6936323
ATGCCGT20100.033.6936344
GCCCACG20600.033.42193618
ACGAGAC20750.033.28876522
CGAGCCC21000.032.78641515
CACGAGA21250.032.71726221
AATCTCG20750.032.52973636
CTCGTAT21200.032.36990439
CCGAGCC21500.032.0239414
TCTCGTA21650.031.69708638
AGCCCAC22250.030.94345517
TCGTATG22300.030.87407540
ATCTCGT22350.030.80500637
CGTATGC22800.030.19701441
GACCTGA21850.030.17126826
GAGCCCA22900.030.16440216
Produced by FastQC (version 0.11.2)