FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_61.341000000052a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_61.341000000052a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679579
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC134440.8004386813600313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14850.042.27194645
TATGCCG17050.036.94946343
ATGCCGT17250.036.26019744
AATCTCG16600.035.9180136
CTCGTAT17800.035.139839
TCGTATG18350.034.45440340
TCTCGTA18100.034.43306438
ACGAGAC18650.034.1414622
GACGCGC401.5605563E-533.7513818
CGAGACC17800.033.7493823
ATCTCGT18950.033.00730537
CCACGAG19300.032.8750420
CCCACGA19600.032.71623219
CACGAGA19600.032.25705721
CGTATGC19550.032.2244741
GCCCACG19700.032.20944218
ACCTGAT19400.030.73488427
GACCTGA19650.030.4583626
CGACGCG453.490816E-530.00122817
CCCGACG300.005148391729.99944519
Produced by FastQC (version 0.11.2)