FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_60.34100000005293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_60.34100000005293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625381
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC49390.30386721636342495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6850.041.71341745
TATGCCG8450.033.81501843
ATGCCGT8650.032.77306444
CGAGACC8400.031.60666723
CCACGAG9400.031.35591520
ACGAGAC9400.031.11655622
TCTCGTA9000.030.99953538
AATCTCG8650.030.9532936
GCCCACG9400.030.87909718
ATCTCGT9200.030.5701937
CTCGTAT9200.030.32562839
CCCACGA9650.030.07634219
AGTCCGC300.00514670830.00139618
CGAGCCC10400.027.91080915
CACGAGA10550.027.724721
TCGTATG10400.027.47554640
CCGAGCC11350.025.77291714
AGCCCAC11950.024.28983117
GACCTGA10850.024.26230826
GAGCCCA12000.024.1893716
Produced by FastQC (version 0.11.2)