FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_59.34100000005286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_59.34100000005286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521484
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG250.002107269636.00018717
GGGAGCG300.005147646630.0001565
CACGCGG852.0372681E-1029.11779617
ACACGCG903.965397E-1027.50104316
CGACGGT1152.6557245E-1023.478387
ACGACGG1152.6557245E-1023.478386
TGCCGTC1850.023.10746645
CGCGGGG1003.6474376E-822.49937419
ACGCGGG1056.110713E-821.4286818
ACGGTAT1301.2278178E-920.7693379
GCGGGGC1109.9924364E-820.4539820
TCTCCGA19950.020.41296845
GACGGTA1452.1827873E-1020.1725168
TATGCCG2150.019.88317143
TCCGACC1151.5966543E-719.56467219
ACCGTCG1102.2568456E-618.4091858
GTATCAA33350.017.8833541
CATCTCC62300.017.73225245
ATCTCCG31600.017.51533745
CGGGACG901.8661056E-417.50008817
Produced by FastQC (version 0.11.2)