FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_58.34100000005279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_58.34100000005279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63760
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC890.13958594730238394RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC670.10508155583437892RNA PCR Primer, Index 23 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGGGC206.98321E-445.03
CCGAGCC401.5376574E-533.7514
TCGTATG401.5376574E-533.7540
TCCGAGC453.4399338E-530.013
CACGAGA453.4399338E-530.021
TATGCCG453.4399338E-530.043
TCTCGTA453.4399338E-530.038
CGAGCCC453.4399338E-530.015
ACCATGC300.00511129429.9999988
AGCCCAC602.3588836E-729.99999817
GCCCACG554.0844297E-628.63636418
CGTATGC507.05469E-527.041
CTCGTAT507.05469E-527.039
CCCACGA507.05469E-527.019
ATGCCGT507.05469E-527.044
ACGAGAC507.05469E-527.022
CCACGAG507.05469E-527.020
TGCCGTC507.05469E-527.045
ATCTCGT551.3485114E-424.54545437
TCTCCGA551.3485114E-424.54545411
Produced by FastQC (version 0.11.2)