##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_57.3410000000526c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12616 Sequences flagged as poor quality 0 Sequence length 51 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.029486366518704 38.0 38.0 38.0 32.0 38.0 2 36.02045022194039 38.0 38.0 38.0 32.0 38.0 3 36.00245719720989 38.0 32.0 38.0 32.0 38.0 4 36.26696258719087 38.0 38.0 38.0 32.0 38.0 5 36.17636334812936 38.0 38.0 38.0 32.0 38.0 6 38.029169308814204 40.0 38.0 40.0 32.0 40.0 7 37.97186112872543 40.0 38.0 40.0 32.0 40.0 8 37.88482878883957 40.0 38.0 40.0 32.0 40.0 9 37.94562460367787 40.0 38.0 40.0 32.0 40.0 10 37.91336398224477 40.0 38.0 40.0 32.0 40.0 11 37.923034242232085 40.0 38.0 40.0 32.0 40.0 12 37.821655041217504 40.0 38.0 40.0 32.0 40.0 13 37.8858592263792 40.0 38.0 40.0 32.0 40.0 14 37.81848446417248 40.0 38.0 40.0 32.0 40.0 15 37.78146797717184 40.0 38.0 40.0 32.0 40.0 16 37.72154407102093 40.0 38.0 40.0 32.0 40.0 17 37.671607482561825 40.0 38.0 40.0 32.0 40.0 18 37.716708941027264 40.0 38.0 40.0 32.0 40.0 19 37.73105580215599 40.0 38.0 40.0 32.0 40.0 20 37.80152187698161 40.0 38.0 40.0 32.0 40.0 21 37.775206087507925 40.0 38.0 40.0 32.0 40.0 22 37.7469086873811 40.0 38.0 40.0 32.0 40.0 23 37.75451807228916 40.0 38.0 40.0 32.0 40.0 24 37.712111604311986 40.0 38.0 40.0 32.0 40.0 25 37.813728598604946 40.0 38.0 40.0 32.0 40.0 26 37.66106531388713 40.0 38.0 40.0 32.0 40.0 27 37.62547558655675 40.0 38.0 40.0 32.0 40.0 28 37.59844641724794 40.0 38.0 40.0 32.0 40.0 29 37.584020291693086 40.0 38.0 40.0 32.0 40.0 30 37.589965123652505 40.0 38.0 40.0 32.0 40.0 31 37.52655358275206 40.0 38.0 40.0 32.0 40.0 32 37.544625871908686 40.0 38.0 40.0 32.0 40.0 33 37.51743817374762 40.0 38.0 40.0 32.0 40.0 34 37.575301204819276 40.0 38.0 40.0 32.0 40.0 35 37.5856055802156 40.0 38.0 40.0 32.0 40.0 36 37.48533608116677 40.0 38.0 40.0 32.0 40.0 37 37.47962904248573 40.0 38.0 40.0 32.0 40.0 38 37.4996829422955 40.0 38.0 40.0 32.0 40.0 39 37.4962745719721 40.0 38.0 40.0 32.0 40.0 40 37.53780913126189 40.0 38.0 40.0 32.0 40.0 41 37.50142675967026 40.0 38.0 40.0 32.0 40.0 42 37.4320703868104 40.0 38.0 40.0 32.0 40.0 43 37.52924857324033 40.0 38.0 40.0 32.0 40.0 44 37.3967184527584 40.0 38.0 40.0 32.0 40.0 45 37.35946417247939 40.0 38.0 40.0 32.0 40.0 46 37.35256816740647 40.0 38.0 40.0 32.0 40.0 47 37.36842105263158 40.0 38.0 40.0 32.0 40.0 48 37.36469562460368 40.0 38.0 40.0 32.0 40.0 49 37.36057387444515 40.0 38.0 40.0 32.0 40.0 50 37.31491756499683 40.0 38.0 40.0 32.0 40.0 51 36.660351934051995 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.9495903862420292 2201 2 1.6640414773473893 2201 3 1.1546948451169854 2201 4 1.872004411270602 2201 5 0.4350527235025581 2201 6 1.4550790782885414 2201 7 1.5934416951144783 2201 8 1.6129671437422033 2201 9 1.3595844420585834 2201 10 1.7779985908978304 2201 11 1.8454482133773524 2201 12 1.8771901573411043 2201 13 1.8144990165027508 2201 14 1.9364348186551936 2201 15 1.6946364190688428 2201 16 1.9570417478585753 2201 17 1.975061632182694 2201 18 1.833247522873208 2201 19 1.6530930812988984 2201 20 0.31766018035004606 2201 21 1.4863688458001718 2201 22 1.6877689147741322 2201 23 1.864954440577634 2201 24 1.996836550164673 2201 25 2.2290727266978934 2201 26 1.7325679968176857 2201 27 1.76002081231303 2201 28 2.169128018130948 2201 29 1.820154717924808 2201 30 2.2735349304661483 2201 31 2.2051067459631426 2201 32 2.1166684778693465 2201 33 2.209794571730768 2201 34 2.0960228731416706 2201 35 1.7729386348306733 2201 36 1.7739808895255038 2201 37 1.736283192172884 2201 38 1.6967708979557443 2201 39 2.161974948152448 2201 40 2.067933902880597 2201 41 2.4023868067137144 2201 42 1.4300923711778495 2201 43 2.0618236236131153 2201 44 2.640399286215114 2201 45 1.4047501057297467 2201 46 2.106361086377511 2201 47 2.2885966767035413 2201 48 2.1861678656948484 2201 49 2.3744740519595737 2201 50 2.2447680500889433 2201 51 1.818499057415849 1101 1 1.092447529099175 1101 2 1.4735652868711995 1101 3 0.9562821467042824 1101 4 -0.17561463634844898 1101 5 0.10171939016922948 1101 6 0.7566663798758384 1101 7 1.0378861395589212 1101 8 0.8828084135834757 1101 9 0.6452987277728681 1101 10 0.47641128931052634 1101 11 0.5597339276630677 1101 12 0.7819520621030094 1101 13 0.4811656831694222 1101 14 1.5237364059567824 1101 15 1.7898745143069377 1101 16 0.845930636747461 1101 17 -2.5011288440077806 1101 18 0.4681681577938406 1101 19 0.17690260510841682 1101 20 -0.42043505774518763 1101 21 -0.11680575737442922 1101 22 1.67189589890112 1101 23 0.6586052342284319 1101 24 -0.09840154507342191 1101 25 1.0703425679677352 1101 26 1.6055838698335592 1101 27 1.3631954154876382 1101 28 1.3437311927341185 1101 29 1.613805511575606 1101 30 1.3529000098312238 1101 31 1.6812972221536242 1101 32 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1.732237421863104 1209 19 -0.7711493429435308 1209 20 0.42372078641065514 1209 21 1.0823284417597705 1209 22 0.788779015784236 1209 23 1.1477827234059177 1209 24 -0.1698301165019913 1209 25 0.13311313073829467 1209 26 0.5406488048984954 1209 27 -0.5026054503132329 1209 28 0.982259331262263 1209 29 -0.033380635610541276 1209 30 1.2230298799610964 1209 31 1.3566218974782984 1209 32 0.495456356657229 1209 33 1.361309723245924 1209 34 1.26774004485884 1209 35 1.4396053014973376 1209 36 -1.6755140599694442 1209 37 0.9029498588395484 1209 38 0.9290941302789761 1209 39 1.3841971703746694 1209 40 0.5830854180321126 1209 41 0.9680433723702819 1209 42 1.0816075226930053 1209 43 1.2537428155323056 1209 44 2.135348781164609 1209 45 1.8794975804772278 1209 46 1.7730277530441754 1209 47 0.9401118282186971 1209 48 1.7417234212504056 1209 49 1.8189184964040166 1209 50 1.8003236056445004 1209 51 1.919509158425953 1214 1 -0.0027905661389198144 1214 2 -2.050244236938326 1214 3 -1.0913369009147615 1214 4 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0.2107471289095244 1212 11 -0.5054289796050995 1212 12 -1.4736870356413476 1212 13 -0.010062387006016138 1212 14 -0.5138575790056237 1212 15 -0.8492232300539655 1212 16 -1.2944202404455183 1212 17 -0.5863418765892305 1212 18 -0.7223080326823492 1212 19 0.5302860637550353 1212 20 -1.2700591178955634 1212 21 -1.9463796922115222 1212 22 -0.4408860559860983 1212 23 -0.1584373722878638 1212 24 -0.038251169133573626 1212 25 -0.8118629458167277 1212 26 0.07174928336738873 1212 27 -1.9475815268682553 1212 28 -1.1875971280678854 1212 29 -3.1506055159933197 1212 30 -0.5042428473116303 1212 31 -2.882612552282467 1212 32 -2.6552613466920576 1212 33 -0.9831878844095812 1212 34 -2.143743208729674 1212 35 0.33434214360259773 1212 36 -1.237715016907245 1212 37 -1.8075764569499242 1212 38 -2.3675565874243745 1212 39 -0.5631712506779607 1212 40 -2.230311711154485 1212 41 -2.3812389242804315 1212 42 -2.65762692706776 1212 43 -1.3826208208313275 1212 44 0.6520951926478631 1212 45 -1.063086151580194 1212 46 -1.1217090890610848 1212 47 -1.904385779436808 1212 48 -1.8372239471706493 1212 49 -0.7073972930696684 1212 50 -0.7259921838291845 1212 51 -0.8364717028180664 1213 1 -5.994854058202414 1213 2 -3.8359585226526143 1213 3 -1.9008607104385717 1213 4 -2.2391066998405122 1213 5 -0.9538361653863277 1213 6 -2.8782542550447943 1213 7 -3.7398916382188574 1213 8 -5.387032856257797 1213 9 -4.473748891274752 1213 10 -4.444223631324391 1213 11 -6.654551786622644 1213 12 -4.78947650932556 1213 13 -4.685500983497249 1213 14 -2.285787403567028 1213 15 -3.249808025375607 1213 16 -4.598513807696982 1213 17 -2.524938367817306 1213 18 -3.111196921571235 1213 19 -1.013573585367773 1213 20 -2.3490064863166182 1213 21 -2.2358533764220496 1213 22 -2.7011199741147536 1213 23 -5.468378892755695 1213 24 -2.3920523387242127 1213 25 -4.493149495524328 1213 26 -3.156320892071207 1213 27 -4.684423632131413 1213 28 -3.719760870757945 1213 29 -3.6242897265196348 1213 30 -4.670909513978295 1213 31 -5.7393376984813 1213 32 -6.6611092999084285 1213 33 -2.956872094935896 1213 34 -2.9039771268583294 1213 35 -1.7826169207248839 1213 36 -3.614907999363382 1213 37 -3.0970501411604516 1213 38 -4.358784657599813 1213 39 -4.004691718514216 1213 40 -5.1542883193416245 1213 41 -4.76427985995295 1213 42 -3.0699076288221505 1213 43 -3.3826208208313275 1213 44 -2.1373784915626644 1213 45 -2.3174721164924748 1213 46 -4.06030558028916 1213 47 -4.378069989963123 1213 48 -4.369387689860709 1213 49 -4.7366370591515405 1213 50 -2.921898616577721 1213 51 -3.5148342759174795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 2.0 23 15.0 24 21.0 25 46.0 26 68.0 27 94.0 28 127.0 29 162.0 30 224.0 31 282.0 32 316.0 33 388.0 34 466.0 35 568.0 36 767.0 37 1101.0 38 1983.0 39 5985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.463538363982245 11.683576410906785 9.036144578313253 31.81674064679772 2 21.141498216409037 19.072532699167656 30.68569163694015 29.10027744748315 3 22.851934051997464 20.933734939759034 26.236525047558658 29.977805960684844 4 16.883322764743184 14.291376030437538 30.84178820545339 37.983512999365885 5 18.46861128725428 32.347812301838935 31.2143310082435 17.969245402663283 6 38.41946734305643 30.89727330374128 13.229232720355105 17.454026632847178 7 33.14045656309448 27.148065948002536 17.46987951807229 22.24159797083069 8 31.951490171211162 29.343690551680407 16.83576410906785 21.869055168040582 9 30.00951173113507 11.770767279644895 14.838300570703868 43.38142041851617 10 18.666772352568167 24.896956246036776 28.083386176284087 28.352885225110967 11 40.543753963221306 19.33259353202283 18.817374762206722 21.306277742549142 12 26.10177552314521 20.878249841471145 25.103043753963224 27.91693088142042 13 33.18008877615726 16.3998097653773 22.725110970196578 27.694990488268868 14 25.499365884590997 17.247939124920737 21.353836398224477 35.89885859226379 15 29.050412175015854 23.430564362714016 19.52282815472416 27.996195307545975 16 28.59067850348763 22.923272035510465 20.814838300570703 27.6712111604312 17 28.170577045022192 22.907419150285353 20.577045022194042 28.344958782498413 18 28.91231964483907 23.077532899952434 20.548596797209452 27.46155065799905 19 30.342422320862394 20.973367152821815 20.569118579581485 28.115091946734307 20 29.961953075459736 20.862396956246037 21.2428662016487 27.93278376664553 21 29.565630944831963 21.116043119847813 20.600824350031704 28.717501585288524 22 29.72415979708307 21.385542168674696 20.0856055802156 28.804692454026632 23 27.93278376664553 21.536144578313255 21.3142041851617 29.21686746987952 24 27.599873176918198 21.70259987317692 21.87698161065314 28.820545339251748 25 28.70957514267597 21.48858592263792 21.837349397590362 27.964489537095748 26 29.002853519340523 21.147748890298036 21.972098922003806 27.877298668357643 27 28.804692454026632 22.495244134432465 21.33798351299937 27.362079898541538 28 28.10716550412175 22.8994927076728 20.109384908053265 28.88395688015219 29 28.368738110336082 23.145212428662017 21.22701331642359 27.259036144578314 30 29.161382371591632 22.80437539632213 20.458148383005707 27.576093849080536 31 27.964489537095748 21.33005707038681 22.70133164235891 28.00412175015853 32 28.37666455294864 22.090995561192138 21.41724793912492 28.115091946734307 33 27.837666455294862 20.584971464806596 22.201965757767912 29.375396322130626 34 27.33037412809131 21.322130627774253 23.129359543436905 28.218135700697527 35 27.56024096385542 21.3142041851617 23.113506658211794 28.012048192771083 36 28.527266962587188 20.370957514267594 22.3287888395688 28.772986683576413 37 27.140139505389982 21.274571972098922 23.509828788839567 28.075459733671526 38 27.116360177552313 19.74476854787571 23.636651870640456 29.50221940393152 39 25.554850982878886 20.370957514267594 24.064679771718453 30.00951173113507 40 27.037095751426758 18.52409638554217 25.89568801521877 28.543119847812303 41 26.04629042485732 19.213696892834495 25.808497146480658 28.931515535827522 42 25.72130627774255 18.90456563094483 26.395053899809767 28.979074191502853 43 23.509828788839567 18.80152187698161 27.694990488268868 29.993658845909955 44 22.859860494610018 19.879518072289155 28.170577045022192 29.090044388078628 45 22.201965757767912 18.26252377932784 30.1284083703234 29.40710209258085 46 22.376347495244133 19.142358909321498 28.662016487000635 29.819277108433734 47 20.87032339885859 19.784400760938492 30.960684844641722 28.384590995561194 48 20.862396956246037 20.077679137603045 29.77964489537096 29.280279010779964 49 20.82276474318326 21.425174381737477 28.891883322764745 28.86017755231452 50 19.562460367786937 21.33005707038681 30.02536461636018 29.082117945466074 51 19.29296131896005 21.520291693088144 27.805960684844642 31.380786303107165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 2.0 13 1.0 14 0.0 15 3.5 16 7.0 17 8.5 18 10.0 19 11.5 20 13.0 21 12.0 22 11.0 23 9.0 24 7.0 25 11.0 26 10.0 27 5.0 28 5.5 29 6.0 30 10.0 31 14.0 32 22.5 33 31.0 34 44.0 35 57.0 36 60.5 37 64.0 38 92.5 39 121.0 40 140.0 41 159.0 42 191.0 43 223.0 44 286.0 45 349.0 46 462.0 47 575.0 48 726.0 49 877.0 50 1024.0 51 1171.0 52 1294.0 53 1417.0 54 1393.0 55 1369.0 56 1329.0 57 1289.0 58 1235.0 59 1181.0 60 1121.5 61 1062.0 62 952.5 63 843.0 64 743.0 65 643.0 66 531.0 67 419.0 68 345.0 69 271.0 70 209.0 71 147.0 72 123.0 73 99.0 74 88.5 75 65.5 76 53.0 77 35.0 78 17.0 79 10.5 80 4.0 81 3.5 82 3.0 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007926442612555484 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.01585288522511097 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 12616.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.6846768427879 #Duplication Level Percentage of deduplicated Percentage of total 1 92.03593712760055 82.54213279211999 2 6.205816319447317 11.131332623106639 3 1.1709500369681076 3.150488269936058 4 0.2888122761694259 1.036081426259399 5 0.13704070176702626 0.614522552614231 6 0.07455669041379273 0.40119556117372696 7 0.024831189515113525 0.15588840450994856 8 0.024824285536581184 0.17810864209611016 9 0.0 0.0 >10 0.037231372582090534 0.7902497281838977 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 29 0.22986683576410905 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 29 0.22986683576410905 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 13 0.1030437539632213 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.007926442612555484 17 0.0 0.0 0.007926442612555484 18 0.0 0.0 0.01585288522511097 19 0.0 0.0 0.01585288522511097 20 0.0 0.0 0.01585288522511097 21 0.0 0.0 0.023779327837666456 22 0.0 0.0 0.03170577045022194 23 0.0 0.0 0.03170577045022194 24 0.0 0.0 0.03963221306277743 25 0.0 0.0 0.0554850982878884 26 0.0 0.0 0.06341154090044387 27 0.0 0.0 0.09511731135066583 28 0.0 0.0 0.1664552948636652 29 0.0 0.0 0.3883956880152188 30 0.0 0.0 0.7133798351299937 31 0.0 0.0 1.014584654407102 32 0.0 0.0 1.4743183259353203 33 0.0 0.0 2.1322130627774256 34 0.0 0.0 2.8693722257450855 35 0.0 0.0 3.709575142675967 36 0.0 0.0 4.882688649334179 37 0.0 0.0 6.5630944831959415 38 0.0 0.0 9.04407102092581 39 0.0 0.0 12.18294229549778 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 175 6.487535E-7 14.142857 45 TTATACA 120 0.0015038165 13.124999 45 TCTTATA 210 6.802138E-5 10.714286 44 CTCTTAT 230 1.7119553E-4 9.782608 43 TCTCTTA 265 7.098357E-4 8.490566 42 GTCTCTT 275 0.0010262476 8.181818 41 TGTCTCT 305 0.0028531519 7.3770494 40 CTGTCTC 325 0.0053094164 6.9230766 39 >>END_MODULE