FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_57.3410000000526c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_57.3410000000526c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC290.22986683576410905No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC290.22986683576410905RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC130.1030437539632213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1756.487535E-714.14285745
TTATACA1200.001503816513.12499945
TCTTATA2106.802138E-510.71428644
CTCTTAT2301.7119553E-49.78260843
TCTCTTA2657.098357E-48.49056642
GTCTCTT2750.00102624768.18181841
TGTCTCT3050.00285315197.377049440
CTGTCTC3250.00530941646.923076639
Produced by FastQC (version 0.11.2)