FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_56.34100000005250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_56.34100000005250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC272.406417112299465No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC121.06951871657754No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC40.35650623885918004RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC40.35650623885918004No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC40.35650623885918004No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC30.267379679144385No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30.267379679144385No Hit
CTTATACACATCTCCGAGCCACGAGACCTCTACTTATCTCGTATGCCGTCT20.17825311942959002No Hit
AGACTACCGTTTGTGCATGGCTAAAGACCGTGGTGATTTTATAGCATCCTG20.17825311942959002No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC20.17825311942959002No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)