FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_55.34100000005243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_55.34100000005243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443047
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAAC17180.11905364135748871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1350.034.99900445
GCGAGCT300.005146662230.00122613
CGCGGTC300.005148406629.99914710
TATGCCG1600.029.53040743
ACGATCG1450.029.48396327
CGATCGT1500.028.50116328
CTCGTAT1800.026.2492539
GATCGTG1550.026.13009829
TATCTCG1800.023.74932336
GCCCACG2000.023.62596518
CCCACGA2000.023.62432919
ATGCCGT2000.023.62432944
ATCGTGT1750.023.14380330
ACGAGAC2050.023.04812422
CCACGAG2050.023.04812420
GAACCGC500.002265262922.4993616
ACGCGCT500.002265262922.49936143
GAGACGA2350.022.02217524
CGCTATT2250.022.00089828
GACGATC1850.021.89278626
Produced by FastQC (version 0.11.2)