FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_54.34100000005236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_54.34100000005236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC491.6257465162574651RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC210.696748506967485RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC200.6635700066357001No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC150.4976775049767751No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC120.3981420039814201No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC100.33178500331785005RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC90.29860650298606506No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC80.26542800265428No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC70.232249502322495No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC50.16589250165892502No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC205.9667096E-445.045
CGAGACG205.9667096E-445.023
ATCTCCG250.001790269336.010
CATCTCC250.001790269336.09
ATGCCGT250.001790269336.044
CCGAGCC250.001790269336.014
TCTCCGA250.001790269336.011
TCCGAGC250.001790269336.013
CTCCGAG250.001790269336.012
GTATGCC300.00437985229.99999842
ATCTCGT300.00437985229.99999837
GCCCACG300.00437985229.99999818
CCACGAG300.00437985229.99999820
TATGCCG300.00437985229.99999843
GAGCCCA300.00437985229.99999816
CACGAGA300.00437985229.99999821
CCCACGA300.00437985229.99999819
ACATCTC300.00437985229.9999988
TCTCGTA300.00437985229.99999838
ACGAGAC300.00437985229.99999822
Produced by FastQC (version 0.11.2)