FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_53.34100000005229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_53.34100000005229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537248
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC53560.34841482961760234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6650.037.8948245
TATGCCG7500.033.5989843
CTCGTAT8050.031.58289339
CGATCGT7600.030.19645128
ACGATCG7550.030.09842527
GATCGTG7600.029.90040829
ATCGTGT7650.029.7049830
ATGCCGT8550.029.4727944
TCTCGTA8900.028.8193538
TCGTATG8850.028.72794240
TATCTCG8550.028.4201936
GAGACGA8100.028.33247424
CGAGACG8600.028.25495723
CCCACGA9300.027.82173319
ACGAGAC9450.027.38012122
CGTGTAT8250.027.27189832
GCCCACG9450.027.14203318
CCACGAG9650.027.0458120
GACGATC8350.026.9452926
TCGTGTA8450.026.89267531
Produced by FastQC (version 0.11.2)