FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_52.3410000000521c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_52.3410000000521c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522441
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCCG406.167571E-428.1246130
ATACCGC500.002264695522.5004276
CATCTCC24900.022.22860745
CTACTCG754.6043868E-520.99970828
ATCTCCG7750.019.74166345
TATACCG706.984003E-419.286085
TATGCCG1051.4463913E-619.28544643
GTATCAA10850.019.081211
AGTATCG851.2045918E-418.5297645
GGTATCA4900.018.3701081
CGAGCCC1354.2738975E-818.33368115
TCTCCGA4050.018.33307845
TAGGGCG1507.2705006E-917.9997531
TGCCGTC1153.4506083E-617.60845245
TCAACGC10850.017.2117424
ATATGTA7250.017.0715311
TAATACT7150.016.9927734
ACCGTTC1601.6141712E-816.8753212
GCGGAAT800.001711348216.87476532
GACCGTT1752.6921043E-916.71460311
Produced by FastQC (version 0.11.2)