FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_51.3410000000520f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_51.3410000000520f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252618
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1350.029.9996343
TGCCGTC1350.029.9996345
AATAGCG406.1669043E-428.1246515
CGAGCCC1600.025.31218515
GCCCACG1700.023.82323518
CCCACGA1700.023.82323519
ACGAGAC1900.023.6848622
TATCTCG1750.021.85687336
ACGATCG1401.364242E-1020.892627
GTCGGTT654.23888E-420.76897212
AGATCGG550.00393701520.45429218
TACGAGT550.00393701520.4542928
GTATCAA9000.020.25461
ATCGTGT1452.1827873E-1020.17216330
CGTAATC1251.7671482E-819.80054522
CATCTCC29800.019.78163345
CCGAGCC2100.019.28547514
ACATCTC49450.019.20097545
ATCTCCG10250.019.09732445
TGGACGT1302.7732312E-819.03822518
Produced by FastQC (version 0.11.2)