FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_50.341000000051f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_50.341000000051f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225329
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG300.00513870629.99985112
TACACTC300.00513870629.9998515
ACTGCTA406.150821E-428.124868
GTATCAA755.585207E-827.0058611
TCTCCGA1250.026.99986545
AAGACCG450.001223530724.9998765
TACGAGG450.001223530724.9998762
ATCTCCG1551.8189894E-1221.77408844
ACGGGGC855.0028466E-621.1763675
GTGTAGT654.2174887E-420.7737391
CGTGCAC600.00649482418.7499073
AGTACGG2900.016.2930222
GAGTACG3050.016.233031
TCAACGC1257.572098E-616.1999193
TACGGGT850.002556674415.8822754
CCGCTCC850.002556674415.8822759
CTCATGG850.002556674415.8822752
ACAGTGC1156.744154E-515.6520978
AGCACCG900.003741582214.9999265
TATCAAC1402.2143808E-514.4642142
Produced by FastQC (version 0.11.2)