FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_5.34100000004eea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_5.34100000004eea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2478
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC381.533494753833737No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC341.3720742534301855No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC240.9685230024213075No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC200.8071025020177561No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC190.7667473769168685RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC180.7263922518159807No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC160.645682001614205RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.2824858757062147No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC60.24213075060532688RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT60.24213075060532688No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC50.20177562550443903No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC30.12106537530266344No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT30.12106537530266344No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT30.12106537530266344No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC252.8649249E-545.017
GTATGCC252.8649249E-545.042
ATCTCCG252.8649249E-545.010
GCCCACG252.8649249E-545.018
TGCCGTC252.8649249E-545.045
CCACGAG252.8649249E-545.020
TATGCCG252.8649249E-545.043
CATCTCC252.8649249E-545.09
GAGCCCA252.8649249E-545.016
CACGAGA252.8649249E-545.021
CCCACGA252.8649249E-545.019
TCTCGTA252.8649249E-545.038
ACGAGAC252.8649249E-545.022
ATGCCGT252.8649249E-545.044
CGTATGC252.8649249E-545.041
CGAGCCC252.8649249E-545.015
CCGAGCC252.8649249E-545.014
TCGTATG252.8649249E-545.040
TCCGAGC252.8649249E-545.013
CTCGTAT252.8649249E-545.039
Produced by FastQC (version 0.11.2)