FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_49.341000000051e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_49.341000000051e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253966
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1000.036.0266381
GTTACTC250.002104522735.998275
AACCCGT300.005141007729.9985566
TAACCTC300.005141007729.9985565
ATATGTA406.1262405E-428.1458151
CAGCGCG450.001224268124.9987969
TATGTAA450.001224268124.9987962
GTTTTAA500.002250094422.516651
GTATCAA1950.021.93931
ATGTAAG550.00392932320.453563
ACGGGAG1051.4371908E-619.2847865
GGATACA600.006469162618.7638761
ACGAGAT600.0064912818.7527928
GATCCGC600.006498666518.7490986
CCATTAC600.006498666518.7490983
CGGGGGG1253.806108E-717.9991346
GGCAGCG800.001706443516.874197
AGTACGG5300.016.1312982
GAGTACG5200.016.0214631
CCTACCC850.002558614115.8815883
Produced by FastQC (version 0.11.2)