FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_48.341000000051d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_48.341000000051d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174546
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.028.8657511
GTATCAA18900.025.6065981
TATAATA500.002264402622.5004045
CGCCTAA550.0039371520.45404222
CAACGCA24700.019.2209935
AACGCAG24950.018.9382156
TCAACGC25400.018.7790664
GCGTCGA600.00650990318.75033611
CGCAGAG25100.018.7353978
TATCAAC25750.018.5246052
ACGCAGA25950.018.2084187
TAGACCG1002.0463804E-518.0003245
ATCAACG27000.017.6662313
CGTACGG800.001710669316.8753039
AGAGTAC28500.016.65819511
TATGCCG1501.4231955E-716.49959443
CAGAGTA28700.016.46370910
GCAGAGT29000.016.2158099
GCGTACG850.002564902215.8826388
CTCGTAT1855.6206773E-915.81042139
Produced by FastQC (version 0.11.2)