FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_47.341000000051ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_47.341000000051ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369374
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30550.8270749971573528No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG5410.14646401749987817No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT4410.11939118616903194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4100.038.4186845
TTGTGCG250.002105572535.99891720
GCTCCCC250.002105572535.99891726
CCGTCTT606.4010237E-933.75355517
GCCGTCT606.4010237E-933.75355516
ATGCCGT4600.033.7489844
TATGCCG4750.032.68322843
CTCGTAT5000.031.04906739
CGTATGC5100.030.44026241
GTATGCC5100.030.44026242
TCGTATG5150.030.14472440
AGACCGC300.005143546529.99909621
GTGCGTT300.005143546529.99909622
TCTCGTA5300.029.29157338
ATCTCGT5700.027.23602337
CCACGAG5550.027.16134520
CCCACGA5600.026.91883519
AATCTCG5200.026.82611536
GCCCACG5650.026.68422718
ACGAGAC5800.026.37851722
Produced by FastQC (version 0.11.2)