FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_46.341000000051bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_46.341000000051bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213604
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT751.7043931E-930.018
TATGCCG2500.029.69877443
TGCCGTC2350.029.68085145
CCCACGA2550.029.1164519
CTAACGG554.1665244E-628.6351834
GACCGCG803.3924152E-928.1273215
CACCGCA406.1678304E-428.1238429
GGACCGC856.4774213E-926.47276914
ATGCCGT2800.025.71322644
CTCGTAT2750.025.36259339
GCCCACG2950.025.16949318
CCACGAG3150.024.9989720
ACGAGAC3050.024.34325822
AATCTCG2900.023.27490436
CGTCATG500.00226359822.50185412
GCGGTTC1003.646346E-822.49907319
GGGCGTT602.4683072E-422.49907333
CGAGACA2900.022.49907323
ATCCTCG1401.364242E-1020.8919968
AGTTCCG1151.5955266E-719.56521618
Produced by FastQC (version 0.11.2)