FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_45.341000000051a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_45.341000000051a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331693
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGG356.2489653E-638.57149518
CACGCGG301.1400078E-437.50006517
ACACGCG352.8199673E-432.1441216
TGCCGTC2150.029.30347345
CGCGGGG406.1679573E-428.12399119
TATGCCG2500.026.09906443
ACCGGGT450.001226913524.9991048
CACCGGG450.001226913524.9991047
CTGTGCG450.001226913524.9991049
CGTCCCG1459.094947E-1221.7249914
CGTGCAA1156.7720975E-921.52177611
GGACCGT754.604611E-520.9992476
TGGACCG550.003937678420.4538125
CGGGTGG2050.019.7561317
TCTCCGA19400.019.60128445
ATCTCCG33100.019.3738745
TGCGTCC1757.2759576E-1219.2864712
CCATCCG1051.4464167E-619.2850239
ATGCCGT3350.018.80529444
CCGCCCA600.006510962718.75003211
Produced by FastQC (version 0.11.2)