FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_44.34100000005194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_44.34100000005194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167848
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG207.033003E-445.0004275
TGCCGTC1251.8189894E-1225.19916245
AACGCAT450.001226498725.00023710
CACCGTT551.3681328E-424.5456897
TATGCCG1353.6379788E-1223.33255643
AGTCGGG903.286732E-722.50021221
CCACGAG1551.8189894E-1221.774420
CCCACGA1459.094947E-1221.72341319
TAGCGTA654.2380154E-420.7694267
ACGTAGC550.003936358720.454748
ATGCCGT1552.3646862E-1120.32190344
TGGTTCG1703.6379788E-1219.8531317
GGTTCGT1757.2759576E-1219.28589618
GCCCACG1656.002665E-1119.09109118
GTCGGTT600.00651019918.750187
TACCCCG851.2043737E-418.52958920
ACACCGT851.2043737E-418.5295896
TCGGGTG851.2043737E-418.52958923
ATCTCCG15400.018.40847845
CGTCCGA750.00111016718.00017211