FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_43.34100000005187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_43.34100000005187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043141
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG751.6975864E-623.99969343
CGTCTAT802.9684616E-622.5051081
CATCTCC34450.019.26680845
ATCTCCG15250.019.18008245
TCTAATA1202.4964356E-718.749762
GCGTCAC600.006510583718.7497636
GTATCAA17050.018.4792671
AGGCCGA901.8656506E-417.4997776
GGTTTCG1053.111038E-517.1434620
TTCGGGG1053.111038E-517.1434623
TCTCCGA8800.017.13046345
CTCCGAG5650.017.12367445
TACTAAT1459.6662916E-817.0687472
TATTAGA1358.5816646E-716.66645417
GTTTATA952.814166E-416.582711
GTTTACG952.8193163E-416.5787359
ACGGGAC3000.016.49978816
GGTATCA10800.016.4620691
GCGATTA1104.6354588E-516.3642124
GCCCACG1104.6373618E-516.36342618
Produced by FastQC (version 0.11.2)