FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_42.3410000000517a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_42.3410000000517a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011271
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTCG300.005147356529.99953820
GTATCAA8250.025.3720281
CCCACGA955.531183E-721.31546219
CCCGATC955.531183E-721.31546231
GTATTAC1301.2223609E-920.7761041
TCAACGC9650.020.7509774
CAACGCA9750.020.5381475
ATCAACG9950.020.3514463
TATCAAC10400.020.3362262
AACGCAG9850.020.3296366
TATGCCG1009.054038E-720.24968743
CATCTCC24350.020.23582645
ACGCAGA10100.019.8264277
CGCAGAG10350.019.3475288
CGTTAGG706.9833966E-419.28541815
TAAGACG706.9833966E-419.2854184
GTATAAT951.3118288E-518.9536381
TACAGCG600.00651054618.7497127
ATCTCCG8750.018.25686345
TCGTTAG750.001110212617.99972314
Produced by FastQC (version 0.11.2)