##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 18h_41.3410000000516d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1228
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.90879478827362	38.0	32.0	38.0	32.0	38.0
2	35.207654723127035	38.0	32.0	38.0	32.0	38.0
3	35.122964169381106	38.0	32.0	38.0	32.0	38.0
4	35.236970684039086	38.0	32.0	38.0	32.0	38.0
5	35.52442996742671	38.0	32.0	38.0	32.0	38.0
6	36.93648208469055	38.0	38.0	40.0	32.0	40.0
7	36.8371335504886	38.0	38.0	40.0	32.0	40.0
8	36.63192182410423	38.0	38.0	40.0	32.0	40.0
9	36.93811074918567	38.0	38.0	40.0	32.0	40.0
10	36.58387622149837	38.0	38.0	40.0	32.0	40.0
11	36.442996742671006	38.0	38.0	40.0	32.0	40.0
12	36.32328990228013	38.0	38.0	40.0	32.0	40.0
13	36.63355048859935	38.0	38.0	40.0	32.0	40.0
14	36.431596091205215	38.0	38.0	40.0	32.0	40.0
15	36.61644951140065	38.0	38.0	40.0	32.0	40.0
16	36.50407166123779	38.0	38.0	40.0	32.0	40.0
17	36.42345276872964	38.0	38.0	40.0	32.0	40.0
18	36.292345276872965	38.0	38.0	40.0	32.0	40.0
19	36.50732899022801	38.0	38.0	40.0	32.0	40.0
20	36.1457654723127	38.0	38.0	40.0	32.0	40.0
21	36.36482084690554	38.0	38.0	40.0	32.0	40.0
22	36.527687296416936	38.0	38.0	40.0	32.0	40.0
23	36.26628664495114	38.0	38.0	40.0	32.0	40.0
24	36.381107491856675	38.0	38.0	40.0	32.0	40.0
25	36.432410423452765	38.0	38.0	40.0	32.0	40.0
26	36.593648208469055	38.0	38.0	40.0	32.0	40.0
27	36.34771986970684	38.0	38.0	40.0	32.0	40.0
28	36.12947882736157	38.0	38.0	40.0	27.0	40.0
29	36.06188925081433	38.0	38.0	40.0	27.0	40.0
30	36.13273615635179	38.0	38.0	40.0	27.0	40.0
31	36.017100977198695	38.0	38.0	40.0	27.0	40.0
32	36.08224755700326	38.0	38.0	40.0	27.0	40.0
33	36.05374592833876	38.0	38.0	40.0	27.0	40.0
34	36.27931596091205	38.0	38.0	40.0	27.0	40.0
35	36.30456026058632	38.0	38.0	40.0	32.0	40.0
36	36.02198697068404	38.0	38.0	40.0	27.0	40.0
37	36.057003257328994	38.0	38.0	40.0	27.0	40.0
38	35.93403908794788	38.0	38.0	40.0	27.0	40.0
39	35.83550488599349	38.0	38.0	40.0	27.0	40.0
40	36.0	38.0	38.0	40.0	27.0	40.0
41	35.62459283387622	38.0	38.0	40.0	27.0	40.0
42	35.73534201954397	38.0	38.0	40.0	27.0	40.0
43	35.83550488599349	38.0	38.0	40.0	27.0	40.0
44	36.03745928338762	38.0	38.0	40.0	27.0	40.0
45	35.61970684039088	38.0	38.0	40.0	27.0	40.0
46	35.377035830618894	38.0	38.0	40.0	27.0	40.0
47	35.438925081433226	38.0	32.0	40.0	27.0	40.0
48	35.295602605863195	38.0	32.0	40.0	27.0	40.0
49	35.46416938110749	38.0	38.0	40.0	27.0	40.0
50	35.37459283387622	38.0	32.0	40.0	27.0	40.0
51	34.56026058631922	38.0	32.0	40.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-9.773809523809526
1101	2	2.566137566137563
1101	3	2.8941798941798993
1101	4	2.0396825396825378
1101	5	-0.6865079365079367
1101	6	0.5238095238095255
1101	7	1.6917989417989503
1101	8	2.341269841269849
1101	9	1.9417989417989503
1101	10	2.1190476190476275
1101	11	1.7552910052910065
1101	12	1.8624338624338677
1101	13	-0.07936507936508974
1101	14	0.7248677248677282
1101	15	1.4682539682539755
1101	16	-9.876984126984127
1101	17	2.8809523809523796
1101	18	1.4894179894179942
1101	19	1.4219576719576565
1101	20	-0.4060846560846514
1101	21	2.074074074074076
1101	22	2.455026455026456
1101	23	2.0092592592592524
1101	24	3.6812169312169374
1101	25	-9.731481481481481
1101	26	3.4021164021164054
1101	27	2.5939153439153486
1101	28	3.0780423280423364
1101	29	0.09920634920635507
1101	30	3.1494708994709057
1101	31	2.9246031746031775
1101	32	3.31216931216931
1101	33	3.337301587301596
1101	34	1.4563492063492163
1101	35	2.186507936507944
1101	36	-0.16402116402116462
1101	37	-1.334656084656082
1101	38	1.9100529100529116
1101	39	2.793650793650791
1101	40	3.2103174603174622
1101	41	3.252645502645507
1101	42	-0.03174603174603163
1101	43	2.7619047619047663
1101	44	3.510582010582013
1101	45	2.632275132275133
1101	46	3.3465608465608483
1101	47	1.892857142857146
1101	48	3.367724867724874
1101	49	2.698412698412689
1101	50	2.304232804232811
1101	51	3.80555555555555
2201	1	2.7261904761904745
2201	2	-0.43386243386243706
2201	3	2.8941798941798993
2201	4	-0.9603174603174622
2201	5	2.3134920634920633
2201	6	2.5238095238095255
2201	7	2.6917989417989503
2201	8	1.341269841269849
2201	9	0.9417989417989503
2201	10	-1.8809523809523725
2201	11	-2.2447089947089935
2201	12	-2.1375661375661323
2201	13	-0.07936507936508974
2201	14	1.7248677248677282
2201	15	1.4682539682539755
2201	16	2.6230158730158735
2201	17	2.8809523809523796
2201	18	1.4894179894179942
2201	19	1.4219576719576565
2201	20	2.5939153439153486
2201	21	-4.925925925925924
2201	22	-4.044973544973544
2201	23	1.0092592592592524
2201	24	-2.8187830687830626
2201	25	1.768518518518519
2201	26	3.4021164021164054
2201	27	-0.4060846560846514
2201	28	3.0780423280423364
2201	29	3.099206349206355
2201	30	3.1494708994709057
2201	31	2.9246031746031775
2201	32	3.31216931216931
2201	33	2.337301587301596
2201	34	-1.5436507936507837
2201	35	1.1865079365079438
2201	36	2.8359788359788354
2201	37	2.665343915343918
2201	38	1.9100529100529116
2201	39	-0.20634920634920917
2201	40	2.2103174603174622
2201	41	2.252645502645507
2201	42	2.9682539682539684
2201	43	3.7619047619047663
2201	44	2.510582010582013
2201	45	-1.367724867724867
2201	46	2.3465608465608483
2201	47	-1.107142857142854
2201	48	3.367724867724874
2201	49	2.698412698412689
2201	50	3.304232804232811
2201	51	3.80555555555555
2202	1	-5.7738095238095255
2202	2	-2.933862433862437
2202	3	-0.10582010582010071
2202	4	2.0396825396825378
2202	5	-0.6865079365079367
2202	6	0.5238095238095255
2202	7	-1.3082010582010497
2202	8	-11.158730158730151
2202	9	-2.0582010582010497
2202	10	-11.380952380952372
2202	11	-2.2447089947089935
2202	12	0.8624338624338677
2202	13	-0.07936507936508974
2202	14	1.7248677248677282
2202	15	-4.0317460317460245
2202	16	2.6230158730158735
2202	17	-9.61904761904762
2202	18	0.4894179894179942
2202	19	-2.5780423280423435
2202	20	-10.906084656084651
2202	21	-4.425925925925924
2202	22	-1.5449735449735442
2202	23	-1.9907407407407476
2202	24	-9.818783068783063
2202	25	-6.731481481481481
2202	26	-22.597883597883595
2202	27	-13.906084656084651
2202	28	-13.421957671957664
2202	29	-3.400793650793645
2202	30	-10.350529100529094
2202	31	-3.5753968253968225
2202	32	-6.18783068783069
2202	33	-7.162698412698404
2202	34	-12.043650793650784
2202	35	1.1865079365079438
2202	36	-10.664021164021165
2202	37	-2.334656084656082
2202	38	-2.0899470899470884
2202	39	1.7936507936507908
2202	40	-0.7896825396825378
2202	41	3.252645502645507
2202	42	-9.531746031746032
2202	43	-0.2380952380952337
2202	44	-22.489417989417987
2202	45	-13.867724867724867
2202	46	-3.1534391534391517
2202	47	-10.607142857142854
2202	48	-3.132275132275126
2202	49	-9.801587301587311
2202	50	-0.6957671957671892
2202	51	-8.69444444444445
1102	1	-5.7738095238095255
1102	2	-9.933862433862437
1102	3	-3.1058201058201007
1102	4	-3.4603174603174622
1102	5	2.3134920634920633
1102	6	-2.4761904761904745
1102	7	-9.80820105820105
1102	8	1.341269841269849
1102	9	0.9417989417989503
1102	10	1.1190476190476275
1102	11	-2.2447089947089935
1102	12	0.8624338624338677
1102	13	-0.07936507936508974
1102	14	-4.775132275132272
1102	15	-4.0317460317460245
1102	16	-3.8769841269841265
1102	17	-10.61904761904762
1102	18	-12.010582010582006
1102	19	-5.0780423280423435
1102	20	2.5939153439153486
1102	21	-1.9259259259259238
1102	22	1.4550264550264558
1102	23	1.0092592592592524
1102	24	-9.818783068783063
1102	25	-9.731481481481481
1102	26	-9.097883597883595
1102	27	-3.9060846560846514
1102	28	2.0780423280423364
1102	29	-10.400793650793645
1102	30	-10.350529100529094
1102	31	-3.5753968253968225
1102	32	-10.18783068783069
1102	33	-7.162698412698404
1102	34	0.4563492063492163
1102	35	2.186507936507944
1102	36	-10.664021164021165
1102	37	-11.834656084656082
1102	38	-3.5899470899470884
1102	39	2.793650793650791
1102	40	2.2103174603174622
1102	41	2.252645502645507
1102	42	1.9682539682539684
1102	43	1.7619047619047663
1102	44	-0.4894179894179871
1102	45	-1.367724867724867
1102	46	-3.1534391534391517
1102	47	-10.607142857142854
1102	48	-10.132275132275126
1102	49	-9.801587301587311
1102	50	-3.195767195767189
1102	51	3.80555555555555
2203	1	-6.607142857142858
2203	2	2.566137566137563
2203	3	-3.1058201058201007
2203	4	-8.293650793650794
2203	5	-3.6865079365079367
2203	6	0.5238095238095255
2203	7	-2.3082010582010497
2203	8	-4.325396825396815
2203	9	-6.7248677248677176
2203	10	-6.54761904761904
2203	11	-0.5780423280423292
2203	12	-15.137566137566132
2203	13	-1.4126984126984254
2203	14	-10.608465608465604
2203	15	-9.865079365079357
2203	16	-1.3769841269841265
2203	17	-0.1190476190476204
2203	18	-7.177248677248674
2203	19	-3.5780423280423435
2203	20	-1.4060846560846514
2203	21	-7.259259259259256
2203	22	-6.878306878306876
2203	23	-4.657407407407412
2203	24	0.6812169312169374
2203	25	-2.231481481481481
2203	26	-5.931216931216927
2203	27	-4.072751322751316
2203	28	-1.9219576719576636
2203	29	-0.9007936507936449
2203	30	-7.517195767195762
2203	31	-1.7420634920634868
2203	32	-7.354497354497358
2203	33	-6.996031746031736
2203	34	1.1230158730158806
2203	35	-0.14682539682539186
2203	36	-0.16402116402116462
2203	37	0.665343915343918
2203	38	-1.0899470899470884
2203	39	-5.873015873015877
2203	40	-0.7896825396825378
2203	41	-9.080687830687825
2203	42	-5.6984126984126995
2203	43	-0.2380952380952337
2203	44	1.1772486772486772
2203	45	-0.367724867724867
2203	46	-7.986772486772484
2203	47	-1.107142857142854
2203	48	-5.965608465608458
2203	49	-3.301587301587311
2203	50	-7.029100529100521
2203	51	-0.19444444444444997
1103	1	-0.2738095238095255
1103	2	-0.43386243386243706
1103	3	-0.10582010582010071
1103	4	2.0396825396825378
1103	5	-0.6865079365079367
1103	6	1.5238095238095255
1103	7	2.6917989417989503
1103	8	2.341269841269849
1103	9	1.9417989417989503
1103	10	3.1190476190476275
1103	11	1.7552910052910065
1103	12	1.8624338624338677
1103	13	1.9206349206349103
1103	14	1.7248677248677282
1103	15	2.4682539682539755
1103	16	2.6230158730158735
1103	17	2.8809523809523796
1103	18	2.489417989417994
1103	19	2.4219576719576565
1103	20	-0.4060846560846514
1103	21	2.074074074074076
1103	22	2.455026455026456
1103	23	2.0092592592592524
1103	24	2.6812169312169374
1103	25	2.768518518518519
1103	26	3.4021164021164054
1103	27	1.5939153439153486
1103	28	3.0780423280423364
1103	29	-0.9007936507936449
1103	30	3.1494708994709057
1103	31	2.9246031746031775
1103	32	3.31216931216931
1103	33	2.337301587301596
1103	34	1.4563492063492163
1103	35	2.186507936507944
1103	36	3.8359788359788354
1103	37	2.665343915343918
1103	38	0.9100529100529116
1103	39	2.793650793650791
1103	40	3.2103174603174622
1103	41	2.252645502645507
1103	42	2.9682539682539684
1103	43	2.7619047619047663
1103	44	2.510582010582013
1103	45	2.632275132275133
1103	46	3.3465608465608483
1103	47	2.892857142857146
1103	48	2.367724867724874
1103	49	1.6984126984126888
1103	50	2.304232804232811
1103	51	4.80555555555555
2204	1	2.7261904761904745
2204	2	2.566137566137563
2204	3	2.8941798941798993
2204	4	2.0396825396825378
2204	5	2.3134920634920633
2204	6	0.5238095238095255
2204	7	-4.30820105820105
2204	8	1.341269841269849
2204	9	0.9417989417989503
2204	10	3.1190476190476275
2204	11	0.7552910052910065
2204	12	2.8624338624338677
2204	13	-0.07936507936508974
2204	14	0.7248677248677282
2204	15	1.4682539682539755
2204	16	1.6230158730158735
2204	17	1.8809523809523796
2204	18	0.4894179894179942
2204	19	0.42195767195765654
2204	20	1.5939153439153486
2204	21	1.0740740740740762
2204	22	3.455026455026456
2204	23	3.0092592592592524
2204	24	2.6812169312169374
2204	25	1.768518518518519
2204	26	4.402116402116405
2204	27	1.5939153439153486
2204	28	2.0780423280423364
2204	29	4.099206349206355
2204	30	4.149470899470906
2204	31	3.9246031746031775
2204	32	4.31216931216931
2204	33	3.337301587301596
2204	34	2.4563492063492163
2204	35	3.186507936507944
2204	36	3.8359788359788354
2204	37	0.665343915343918
2204	38	0.9100529100529116
2204	39	1.7936507936507908
2204	40	2.2103174603174622
2204	41	-3.747354497354493
2204	42	-4.031746031746032
2204	43	1.7619047619047663
2204	44	-3.489417989417987
2204	45	1.632275132275133
2204	46	2.3465608465608483
2204	47	3.892857142857146
2204	48	4.367724867724874
2204	49	1.6984126984126888
2204	50	1.3042328042328108
2204	51	-2.19444444444445
2205	1	-3.2738095238095255
2205	2	-3.433862433862437
2205	3	-3.1058201058201007
2205	4	-3.9603174603174622
2205	5	-3.6865079365079367
2205	6	2.5238095238095255
2205	7	1.6917989417989503
2205	8	-4.658730158730151
2205	9	-5.05820105820105
2205	10	-9.880952380952372
2205	11	-5.2447089947089935
2205	12	-5.137566137566132
2205	13	-0.07936507936508974
2205	14	0.7248677248677282
2205	15	1.4682539682539755
2205	16	3.6230158730158735
2205	17	3.8809523809523796
2205	18	2.489417989417994
2205	19	0.42195767195765654
2205	20	3.5939153439153486
2205	21	1.0740740740740762
2205	22	1.4550264550264558
2205	23	3.0092592592592524
2205	24	-3.3187830687830626
2205	25	1.768518518518519
2205	26	2.4021164021164054
2205	27	1.5939153439153486
2205	28	2.0780423280423364
2205	29	2.099206349206355
2205	30	4.149470899470906
2205	31	1.9246031746031775
2205	32	2.31216931216931
2205	33	1.3373015873015959
2205	34	0.4563492063492163
2205	35	3.186507936507944
2205	36	3.8359788359788354
2205	37	0.665343915343918
2205	38	0.9100529100529116
2205	39	1.7936507936507908
2205	40	4.210317460317462
2205	41	2.252645502645507
2205	42	3.9682539682539684
2205	43	1.7619047619047663
2205	44	4.510582010582013
2205	45	-4.367724867724867
2205	46	2.3465608465608483
2205	47	1.892857142857146
2205	48	-3.632275132275126
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1209	15	3.4682539682539755
1209	16	3.6230158730158735
1209	17	2.8809523809523796
1209	18	1.4894179894179942
1209	19	1.4219576719576565
1209	20	2.5939153439153486
1209	21	3.074074074074076
1209	22	2.455026455026456
1209	23	2.0092592592592524
1209	24	3.6812169312169374
1209	25	2.768518518518519
1209	26	4.402116402116405
1209	27	2.5939153439153486
1209	28	3.0780423280423364
1209	29	3.099206349206355
1209	30	4.149470899470906
1209	31	3.9246031746031775
1209	32	4.31216931216931
1209	33	2.337301587301596
1209	34	2.4563492063492163
1209	35	3.186507936507944
1209	36	3.8359788359788354
1209	37	1.665343915343918
1209	38	1.9100529100529116
1209	39	3.793650793650791
1209	40	4.210317460317462
1209	41	4.252645502645507
1209	42	3.9682539682539684
1209	43	3.7619047619047663
1209	44	4.510582010582013
1209	45	3.632275132275133
1209	46	4.346560846560848
1209	47	3.892857142857146
1209	48	3.367724867724874
1209	49	3.698412698412689
1209	50	3.304232804232811
1209	51	5.80555555555555
1214	1	-3.2738095238095255
1214	2	-0.43386243386243706
1214	3	-12.6058201058201
1214	4	2.0396825396825378
1214	5	-0.6865079365079367
1214	6	1.5238095238095255
1214	7	2.6917989417989503
1214	8	-0.6587301587301511
1214	9	-3.5582010582010497
1214	10	2.1190476190476275
1214	11	1.7552910052910065
1214	12	1.8624338624338677
1214	13	-2.0793650793650897
1214	14	1.7248677248677282
1214	15	2.4682539682539755
1214	16	-0.37698412698412653
1214	17	-0.1190476190476204
1214	18	0.4894179894179942
1214	19	-1.5780423280423435
1214	20	2.5939153439153486
1214	21	1.0740740740740762
1214	22	-4.544973544973544
1214	23	-1.9907407407407476
1214	24	2.6812169312169374
1214	25	1.768518518518519
1214	26	0.4021164021164054
1214	27	2.5939153439153486
1214	28	-10.421957671957664
1214	29	0.09920634920635507
1214	30	3.1494708994709057
1214	31	1.9246031746031775
1214	32	2.31216931216931
1214	33	2.337301587301596
1214	34	1.4563492063492163
1214	35	3.186507936507944
1214	36	-0.16402116402116462
1214	37	-10.834656084656082
1214	38	1.9100529100529116
1214	39	-10.70634920634921
1214	40	3.2103174603174622
1214	41	3.252645502645507
1214	42	2.9682539682539684
1214	43	3.7619047619047663
1214	44	0.5105820105820129
1214	45	2.632275132275133
1214	46	3.3465608465608483
1214	47	2.892857142857146
1214	48	3.367724867724874
1214	49	-0.3015873015873112
1214	50	2.304232804232811
1214	51	0.80555555555555
1215	1	2.7261904761904745
1215	2	-0.43386243386243706
1215	3	-0.10582010582010071
1215	4	2.0396825396825378
1215	5	-0.6865079365079367
1215	6	-2.4761904761904745
1215	7	-1.3082010582010497
1215	8	-1.658730158730151
1215	9	1.9417989417989503
1215	10	2.1190476190476275
1215	11	1.7552910052910065
1215	12	2.8624338624338677
1215	13	-2.0793650793650897
1215	14	-10.775132275132272
1215	15	-10.031746031746025
1215	16	-2.8769841269841265
1215	17	-9.61904761904762
1215	18	0.4894179894179942
1215	19	1.4219576719576565
1215	20	-9.906084656084651
1215	21	2.074074074074076
1215	22	2.455026455026456
1215	23	-3.4907407407407476
1215	24	-8.818783068783063
1215	25	-2.731481481481481
1215	26	-2.0978835978835946
1215	27	-9.906084656084651
1215	28	0.07804232804233635
1215	29	-9.400793650793645
1215	30	-10.350529100529094
1215	31	2.9246031746031775
1215	32	-0.6878306878306901
1215	33	-0.6626984126984041
1215	34	-2.5436507936507837
1215	35	2.186507936507944
1215	36	2.8359788359788354
1215	37	1.665343915343918
1215	38	1.9100529100529116
1215	39	1.7936507936507908
1215	40	-0.7896825396825378
1215	41	-2.247354497354493
1215	42	-0.03174603174603163
1215	43	2.7619047619047663
1215	44	0.5105820105820129
1215	45	-0.367724867724867
1215	46	0.3465608465608483
1215	47	2.892857142857146
1215	48	3.367724867724874
1215	49	3.698412698412689
1215	50	1.3042328042328108
1215	51	1.80555555555555
1212	1	2.7261904761904745
1212	2	2.566137566137563
1212	3	-9.6058201058201
1212	4	-3.4603174603174622
1212	5	2.3134920634920633
1212	6	1.5238095238095255
1212	7	2.6917989417989503
1212	8	1.341269841269849
1212	9	1.9417989417989503
1212	10	1.1190476190476275
1212	11	-4.7447089947089935
1212	12	0.8624338624338677
1212	13	0.9206349206349103
1212	14	0.7248677248677282
1212	15	-10.031746031746025
1212	16	-9.876984126984127
1212	17	-3.6190476190476204
1212	18	-11.010582010582006
1212	19	-1.5780423280423435
1212	20	2.5939153439153486
1212	21	2.074074074074076
1212	22	1.4550264550264558
1212	23	1.0092592592592524
1212	24	2.6812169312169374
1212	25	1.768518518518519
1212	26	-10.097883597883595
1212	27	-3.9060846560846514
1212	28	-0.9219576719576636
1212	29	2.099206349206355
1212	30	2.1494708994709057
1212	31	1.9246031746031775
1212	32	2.31216931216931
1212	33	2.337301587301596
1212	34	0.4563492063492163
1212	35	2.186507936507944
1212	36	-1.1640211640211646
1212	37	-2.334656084656082
1212	38	-4.589947089947088
1212	39	-1.2063492063492092
1212	40	2.2103174603174622
1212	41	-10.247354497354493
1212	42	-3.5317460317460316
1212	43	-1.2380952380952337
1212	44	2.510582010582013
1212	45	1.632275132275133
1212	46	3.3465608465608483
1212	47	-1.107142857142854
1212	48	-0.6322751322751259
1212	49	1.6984126984126888
1212	50	-1.6957671957671892
1212	51	3.80555555555555
1213	1	2.7261904761904745
1213	2	-3.433862433862437
1213	3	-3.1058201058201007
1213	4	-3.9603174603174622
1213	5	2.3134920634920633
1213	6	0.5238095238095255
1213	7	1.6917989417989503
1213	8	-4.658730158730151
1213	9	0.9417989417989503
1213	10	3.1190476190476275
1213	11	0.7552910052910065
1213	12	0.8624338624338677
1213	13	1.9206349206349103
1213	14	0.7248677248677282
1213	15	1.4682539682539755
1213	16	1.6230158730158735
1213	17	1.8809523809523796
1213	18	2.489417989417994
1213	19	2.4219576719576565
1213	20	3.5939153439153486
1213	21	1.0740740740740762
1213	22	3.455026455026456
1213	23	1.0092592592592524
1213	24	2.6812169312169374
1213	25	1.768518518518519
1213	26	-3.5978835978835946
1213	27	-4.406084656084651
1213	28	-3.9219576719576636
1213	29	2.099206349206355
1213	30	2.1494708994709057
1213	31	1.9246031746031775
1213	32	-3.68783068783069
1213	33	1.3373015873015959
1213	34	0.4563492063492163
1213	35	1.1865079365079438
1213	36	1.8359788359788354
1213	37	0.665343915343918
1213	38	-5.089947089947088
1213	39	1.7936507936507908
1213	40	-3.7896825396825378
1213	41	-8.747354497354493
1213	42	-9.031746031746032
1213	43	-4.238095238095234
1213	44	-3.489417989417987
1213	45	1.632275132275133
1213	46	2.3465608465608483
1213	47	1.892857142857146
1213	48	-3.632275132275126
1213	49	1.6984126984126888
1213	50	1.3042328042328108
1213	51	-21.19444444444445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	0.0
23	1.0
24	7.0
25	9.0
26	9.0
27	27.0
28	24.0
29	34.0
30	33.0
31	38.0
32	61.0
33	54.0
34	71.0
35	93.0
36	107.0
37	147.0
38	213.0
39	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.644951140065146	16.123778501628664	14.73941368078176	32.491856677524424
2	14.169381107491857	20.928338762214985	43.89250814332247	21.009771986970684
3	15.309446254071663	26.465798045602607	34.20195439739413	24.022801302931597
4	12.133550488599349	23.12703583061889	34.93485342019544	29.80456026058632
5	14.250814332247558	30.211726384364823	39.49511400651466	16.042345276872965
6	27.36156351791531	40.71661237785016	16.449511400651463	15.472312703583063
7	26.62866449511401	31.188925081433226	18.729641693811075	23.452768729641694
8	25.814332247557005	37.214983713355046	18.566775244299674	18.403908794788272
9	24.592833876221498	14.495114006514658	18.973941368078176	41.93811074918567
10	16.938110749185668	29.153094462540718	30.211726384364823	23.697068403908794
11	30.456026058631924	23.37133550488599	25.814332247557005	20.35830618892508
12	20.765472312703583	26.221498371335507	27.68729641693811	25.325732899022803
13	27.19869706840391	19.055374592833875	32.57328990228013	21.172638436482085
14	19.86970684039088	24.9185667752443	24.104234527687296	31.107491856677527
15	20.928338762214985	26.384364820846905	24.592833876221498	28.094462540716613
16	25.0	25.0814332247557	28.990228013029316	20.928338762214985
17	20.928338762214985	32.410423452768725	25.4885993485342	21.172638436482085
18	28.094462540716613	23.37133550488599	25.651465798045603	22.88273615635179
19	26.058631921824105	20.27687296416938	27.524429967426713	26.140065146579804
20	23.04560260586319	24.592833876221498	25.2442996742671	27.11726384364821
21	22.149837133550488	25.570032573289904	25.325732899022803	26.95439739413681
22	25.0814332247557	27.28013029315961	25.895765472312704	21.74267100977199
23	20.27687296416938	23.778501628664493	28.257328990228014	27.68729641693811
24	27.035830618892508	25.977198697068403	27.850162866449512	19.136807817589577
25	23.778501628664493	29.88599348534202	27.850162866449512	18.485342019543975
26	27.035830618892508	25.1628664495114	25.814332247557005	21.986970684039086
27	19.951140065146582	33.14332247557003	24.7557003257329	22.149837133550488
28	18.485342019543975	25.977198697068403	27.36156351791531	28.175895765472315
29	22.63843648208469	27.11726384364821	27.28013029315961	22.964169381107492
30	24.674267100977197	27.524429967426713	26.547231270358306	21.254071661237788
31	26.221498371335507	24.348534201954397	28.257328990228014	21.172638436482085
32	20.684039087947884	28.338762214983714	26.87296416938111	24.104234527687296
33	22.068403908794785	24.592833876221498	32.73615635179153	20.602605863192185
34	24.267100977198698	22.71986970684039	30.211726384364823	22.80130293159609
35	18.403908794788272	25.732899022801302	32.16612377850163	23.697068403908794
36	23.37133550488599	24.5114006514658	29.153094462540718	22.964169381107492
37	17.996742671009773	29.47882736156352	29.80456026058632	22.71986970684039
38	18.485342019543975	22.55700325732899	35.74918566775244	23.208469055374593
39	17.34527687296417	23.941368078175895	30.700325732899024	28.013029315960914
40	16.286644951140065	23.04560260586319	37.54071661237785	23.12703583061889
41	19.706840390879478	23.04560260586319	29.967426710097723	27.28013029315961
42	24.267100977198698	23.37133550488599	28.257328990228014	24.104234527687296
43	17.019543973941367	22.231270358306187	36.156351791530945	24.592833876221498
44	15.716612377850161	26.87296416938111	31.921824104234524	25.4885993485342
45	16.612377850162865	22.39413680781759	36.31921824104234	24.674267100977197
46	20.195439739413683	20.439739413680783	31.27035830618892	28.094462540716613
47	14.65798045602606	22.231270358306187	32.24755700325733	30.863192182410426
48	13.517915309446254	23.37133550488599	31.840390879478825	31.27035830618892
49	19.29967426710098	24.7557003257329	30.456026058631924	25.4885993485342
50	15.472312703583063	22.475570032573287	34.77198697068404	27.28013029315961
51	15.716612377850161	24.429967426710096	29.23452768729642	30.618892508143325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.5
12	1.0
13	1.5
14	2.0
15	4.5
16	7.0
17	8.0
18	9.0
19	10.0
20	11.0
21	9.5
22	8.0
23	6.0
24	4.0
25	3.5
26	4.0
27	5.0
28	7.0
29	9.0
30	9.0
31	9.0
32	15.0
33	21.0
34	25.5
35	30.0
36	32.5
37	35.0
38	56.0
39	77.0
40	87.5
41	98.0
42	118.5
43	139.0
44	136.5
45	134.0
46	130.0
47	126.0
48	130.5
49	135.0
50	111.0
51	87.0
52	103.0
53	119.0
54	82.5
55	46.0
56	39.5
57	33.0
58	27.0
59	21.0
60	20.0
61	19.0
62	17.5
63	16.0
64	12.0
65	8.0
66	6.0
67	4.0
68	4.5
69	5.0
70	4.0
71	3.0
72	2.0
73	1.0
74	0.5
75	0.5
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1228.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.89250814332247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.35212489158717	92.34527687296416
2	0.9540329575021683	1.7915309446254073
3	0.43365134431916735	1.221498371335505
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.08673026886383348	0.5700325732899023
8	0.0	0.0
9	0.0	0.0
>10	0.17346053772766695	4.071661237785016
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	31	2.5244299674267103	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	19	1.5472312703583062	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	7	0.5700325732899023	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	3	0.24429967426710095	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT	3	0.24429967426710095	No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA	3	0.24429967426710095	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	3	0.24429967426710095	RNA PCR Primer, Index 45 (95% over 21bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	3	0.24429967426710095	No Hit
TCTTGTGGCTGCCCATTTCGTATTGATATCCGCCTTCTCCCAAGCTTTTCG	2	0.16286644951140067	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT	2	0.16286644951140067	No Hit
CTCCATGAGGATGCGCTTGTTTTTGAACACATTCCCTTTGACCTTCAGGTA	2	0.16286644951140067	No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG	2	0.16286644951140067	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.16286644951140067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATG	2	0.16286644951140067	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.16286644951140067	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.16286644951140067	No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG	2	0.16286644951140067	No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	2	0.16286644951140067	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA	2	0.16286644951140067	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.0
21	0.0	0.0	0.0
22	0.0	0.0	0.08143322475570032
23	0.0	0.0	0.08143322475570032
24	0.0	0.0	0.08143322475570032
25	0.0	0.0	0.08143322475570032
26	0.0	0.0	0.16286644951140064
27	0.0	0.0	0.16286644951140064
28	0.0	0.0	0.9771986970684039
29	0.0	0.0	2.035830618892508
30	0.0	0.0	3.257328990228013
31	0.0	0.0	4.96742671009772
32	0.0	0.0	6.840390879478828
33	0.0	0.0	8.387622149837133
34	0.0	0.0	9.690553745928339
35	0.0	0.0	10.830618892508143
36	0.0	0.0	12.296416938110749
37	0.0	0.0	14.006514657980455
38	0.0	0.0	16.123778501628664
39	0.0	0.0	18.485342019543975
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	15	0.00991456	44.999996	6
ACGCAGA	15	0.00991456	44.999996	7
TCAACGC	15	0.00991456	44.999996	4
CAGAGTA	15	0.00991456	44.999996	10
AGAGTAC	15	0.00991456	44.999996	11
GCAGAGT	15	0.00991456	44.999996	9
CGCAGAG	15	0.00991456	44.999996	8
ATCAACG	15	0.00991456	44.999996	3
CAACGCA	15	0.00991456	44.999996	5
>>END_MODULE