FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_41.3410000000516d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_41.3410000000516d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC312.5244299674267103No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC191.5472312703583062RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC70.5700325732899023No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC30.24429967426710095No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT30.24429967426710095No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA30.24429967426710095No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC30.24429967426710095RNA PCR Primer, Index 45 (95% over 21bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA30.24429967426710095No Hit
TCTTGTGGCTGCCCATTTCGTATTGATATCCGCCTTCTCCCAAGCTTTTCG20.16286644951140067No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT20.16286644951140067No Hit
CTCCATGAGGATGCGCTTGTTTTTGAACACATTCCCTTTGACCTTCAGGTA20.16286644951140067No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG20.16286644951140067No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16286644951140067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATG20.16286644951140067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16286644951140067No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16286644951140067No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG20.16286644951140067No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG20.16286644951140067No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA20.16286644951140067No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG150.0099145644.9999966
ACGCAGA150.0099145644.9999967
TCAACGC150.0099145644.9999964
CAGAGTA150.0099145644.99999610
AGAGTAC150.0099145644.99999611
GCAGAGT150.0099145644.9999969
CGCAGAG150.0099145644.9999968
ATCAACG150.0099145644.9999963
CAACGCA150.0099145644.9999965
Produced by FastQC (version 0.11.2)