FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_40.34100000005151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_40.34100000005151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271582
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC180356.640719929892261No Hit
TACTAGAGCAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTA11440.4212355752590378No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCT6830.2514894212429395No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA6740.24817550500401353No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAAT6600.2430205241879064No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAAT6180.2275555817395851No Hit
GCCCAATTCTATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCA5700.20988136179864647No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACC5530.2036217422362307No Hit
TTACTAGAGCAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTT5520.20325352932079446No Hit
ATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATG5410.199203187250996No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTC5340.19662569684294248No Hit
GTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATA5000.18410645771811093No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTT4710.1734282831704605No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTA4510.16606402486173605No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA3810.14028912078120054No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG3770.13881626911945563No Hit
TACTAAGGCAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTA3680.13550235288052964No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAAGCACTTTACATA3420.12592881707918788No Hit
ACTCAGAAGCATCCTCTACAAATTACAACTCGGACGTCGAAGACGCCAGAT3290.12114204917851698No Hit
ACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTA3220.11856455877046343No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATA3130.11525064253153743No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG3080.11340957795435633No Hit
CCGTTACTAGAGCAATCCCTGTTGGTTTCTTTTCCTCCGCTTATTGATATG2980.1097274487999941No Hit
GGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAA2920.10751817130737677No Hit
GAGTACGGGTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCAT2910.10714995839194055No Hit
CCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAAGCGGA2900.10678174547650433No Hit
CTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAA2890.10641353256106811No Hit
GTATCAACGCAGAGTACGGGTTGACCTCAAATCAGGTAGGATTACCCGCTG2860.10530889381475945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21500.041.5448145
ATGCCGT21650.041.36089344
TATGCCG22000.040.80515343
CTCGTAT22000.040.49834439
GTATGCC22250.040.44778442
CGTATGC22300.040.25620341
TCTCGTA22200.040.03214638
TACTTAT22300.039.8526332
ATCTCGT22400.039.67471337
TCGTATG22650.039.6341440
TATCTCG22500.039.49838336
CTTATCT22450.039.38591834
ACTTATC22600.039.2240633
CTACTTA22800.039.07734731
TTATCTC22700.039.05126635
ACCTCTA23550.037.64177327
AGACCTC23950.037.1070425
GAGACCT24050.037.04630324
CGAGACC24050.037.04630323
GACCTCT23950.037.013126
Produced by FastQC (version 0.11.2)