FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_4.34100000004ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_4.34100000004ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75250
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC1570.20863787375415282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.159468438538206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.10232558139534884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA403.3838114E-739.40061
ACGGGGG352.7958068E-432.14238416
GTATCAA1450.031.0546591
GTAGCTC300.005117470429.999564
GACTGGG300.005117470429.999567
TACGGGG856.2791514E-926.47019815
TATCAAC1850.024.3239652
GAGTACG1304.7293724E-1122.4996712
TCTGGAG500.002245340722.4996683
ATCAACG2050.021.9508953
AGTACGG1358.0035534E-1121.66634813
AACGCAG2100.021.4282536
TCAACGC2100.021.4282534
CAACGCA2150.020.9299245
GTACGGG1401.2914825E-1020.89254814
ACGCAGA2350.019.1486537
CGCAGAG2350.019.1486538
ACGGGGA600.00645597218.74972516
AGAGTAC2450.018.36707511
CAGAGTA2450.018.36707510
Produced by FastQC (version 0.11.2)