FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_39.34100000005144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_39.34100000005144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090606
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC14400.13203668419209139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCG207.0308684E-445.0029349
TGCCGTC3150.034.9990745
TATGCCG3400.032.42561343
GCCCACG3500.032.14200618
CGAGACC2700.031.66582923
CACGAGA3700.031.62078521
CCACGAG3700.031.01269320
CCCACGA3650.030.821119
ACGAGAC3650.030.821122
TATCTCG3300.030.68100736
CTCGTAT3600.030.6241939
ATGCCGT3750.029.99920544
CCGAGCC3750.029.40056814
CGAGCCC3900.028.84671215
AGCCCAC4000.028.12554617
TCTCGTA3900.027.69157438
ATCTCGT3950.027.34104737
GAGCCCA4500.025.00048416
TCGTATG4350.024.82692740
GACCTCT3550.024.71765526
Produced by FastQC (version 0.11.2)