##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 18h_38.34100000005137.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4144
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.766891891891895	38.0	32.0	38.0	27.0	38.0
2	34.38248069498069	38.0	32.0	38.0	27.0	38.0
3	34.46669884169884	38.0	32.0	38.0	32.0	38.0
4	34.67881274131274	38.0	32.0	38.0	32.0	38.0
5	34.92084942084942	38.0	32.0	38.0	32.0	38.0
6	36.22611003861004	38.0	38.0	40.0	32.0	40.0
7	35.820945945945944	38.0	38.0	40.0	27.0	40.0
8	35.84314671814672	38.0	38.0	40.0	27.0	40.0
9	35.91336872586873	38.0	38.0	40.0	27.0	40.0
10	35.75772200772201	38.0	38.0	40.0	27.0	40.0
11	35.95849420849421	38.0	38.0	40.0	32.0	40.0
12	35.59411196911197	38.0	38.0	40.0	27.0	40.0
13	35.75772200772201	38.0	38.0	40.0	27.0	40.0
14	35.618967181467184	38.0	38.0	40.0	27.0	40.0
15	35.62162162162162	38.0	38.0	40.0	27.0	40.0
16	35.53643822393823	38.0	32.0	40.0	27.0	40.0
17	35.44329150579151	38.0	32.0	40.0	27.0	40.0
18	35.327220077220076	38.0	32.0	40.0	27.0	40.0
19	35.40685328185328	38.0	32.0	40.0	27.0	40.0
20	35.33301158301158	38.0	32.0	40.0	27.0	40.0
21	35.45680501930502	38.0	32.0	40.0	27.0	40.0
22	35.409507722007724	38.0	32.0	40.0	27.0	40.0
23	35.41795366795367	38.0	32.0	40.0	27.0	40.0
24	35.46645752895753	38.0	32.0	40.0	27.0	40.0
25	35.27413127413127	38.0	32.0	40.0	27.0	40.0
26	35.25144787644788	38.0	32.0	40.0	27.0	40.0
27	35.06998069498069	38.0	32.0	40.0	27.0	40.0
28	35.062982625482626	38.0	32.0	40.0	27.0	40.0
29	34.976351351351354	38.0	32.0	40.0	27.0	40.0
30	35.050916988416986	38.0	32.0	40.0	27.0	40.0
31	34.80912162162162	38.0	32.0	40.0	27.0	40.0
32	35.05381274131274	38.0	32.0	40.0	27.0	40.0
33	34.943532818532816	38.0	32.0	40.0	27.0	40.0
34	34.991554054054056	38.0	32.0	38.0	27.0	40.0
35	34.987210424710426	38.0	32.0	38.0	27.0	40.0
36	34.860279922779924	38.0	32.0	40.0	27.0	40.0
37	34.748310810810814	38.0	32.0	38.0	27.0	40.0
38	34.986486486486484	38.0	32.0	38.0	27.0	40.0
39	35.031129343629345	38.0	32.0	40.0	27.0	40.0
40	35.116554054054056	38.0	32.0	40.0	27.0	40.0
41	35.02823359073359	38.0	32.0	40.0	27.0	40.0
42	35.03378378378378	38.0	32.0	40.0	27.0	40.0
43	35.25723938223938	38.0	32.0	38.0	27.0	40.0
44	34.991795366795365	38.0	32.0	40.0	27.0	40.0
45	35.03281853281853	38.0	32.0	40.0	27.0	40.0
46	34.7765444015444	38.0	32.0	40.0	27.0	40.0
47	34.86510617760618	38.0	32.0	40.0	27.0	40.0
48	34.69184362934363	38.0	32.0	38.0	27.0	40.0
49	34.73021235521235	38.0	32.0	40.0	27.0	40.0
50	34.70945945945946	38.0	32.0	38.0	27.0	40.0
51	34.159507722007724	38.0	32.0	38.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2201	1	1.9501674107142861
2201	2	-0.884021577380949
2201	3	1.7166108630952337
2201	4	-2.4023251488095276
2201	5	-0.13967633928570677
2201	6	-2.4496093750000014
2201	7	1.5687872023809604
2201	8	3.3526227678571416
2201	9	3.26809895833334
2201	10	-0.4465959821428598
2201	11	0.15593377976190936
2201	12	2.7681733630952507
2201	13	0.13813244047620543
2201	14	-4.722619047619055
2201	15	-2.5740141369047578
2201	16	1.7116071428571473
2201	17	-1.204538690476177
2201	18	-1.4856584821428598
2201	19	1.912611607142857
2201	20	0.8044828869047578
2201	21	2.9864397321428555
2201	22	-1.971130952380939
2201	23	-3.484486607142859
2201	24	2.4434151785714278
2201	25	-6.2452938988095354
2201	26	0.5894531249999915
2201	27	1.4620349702380864
2201	28	3.233649553571439
2201	29	-0.3796502976190581
2201	30	0.4057849702380949
2201	31	2.01874999999999
2201	32	-3.386272321428578
2201	33	-2.72622767857143
2201	34	2.222154017857143
2201	35	1.7594494047619094
2201	36	3.536160714285714
2201	37	1.7164620535714263
2201	38	3.4867373511904702
2201	39	2.2627604166666657
2201	40	-1.2549851190476176
2201	41	1.6050409226190538
2201	42	2.9444940476190524
2201	43	3.0404947916666814
2201	44	2.131547619047616
2201	45	4.427566964285717
2201	46	1.6895647321428626
2201	47	5.002641369047623
2201	48	-1.6336681547618994
2201	49	-1.4306919642857068
2201	50	1.0414992559523668
2201	51	-1.226525297619041
1101	1	-0.6498325892857153
1101	2	2.915978422619048
1101	3	3.1166108630952323
1101	4	3.7976748511904717
1101	5	2.4603236607142946
1101	6	3.4837239583333357
1101	7	3.3021205357142946
1101	8	3.4859561011904745
1101	9	3.401432291666673
1101	10	3.0867373511904788
1101	11	-0.6773995535714263
1101	12	1.6681733630952493
1101	13	4.271465773809538
1101	14	2.6107142857142804
1101	15	3.492652529761905
1101	16	4.311607142857149
1101	17	2.3287946428571615
1101	18	2.0476748511904788
1101	19	1.9792782738095198
1101	20	-1.1955171130952422
1101	21	-1.7468936011904788
1101	22	0.12886904761906237
1101	23	-5.184486607142858
1101	24	-5.156584821428574
1101	25	2.6880394345238017
1101	26	-0.6438802083333428
1101	27	3.3953683035714235
1101	28	3.9669828869047734
1101	29	3.487016369047609
1101	30	3.072451636904759
1101	31	0.11874999999999147
1101	32	-2.71960565476191
1101	33	-2.192894345238095
1101	34	0.0888206845238102
1101	35	2.6927827380952465
1101	36	3.469494047619051
1101	37	4.383128720238091
1101	38	4.086737351190472
1101	39	-0.7705729166666728
1101	40	3.6116815476190496
1101	41	3.338374255952388
1101	42	3.6111607142857167
1101	43	4.107161458333344
1101	44	3.9982142857142833
1101	45	3.5609002976190496
1101	46	4.8228980654761955
1101	47	4.80264136904762
1101	48	2.266331845238099
1101	49	3.3026413690476275
1101	50	3.974832589285704
1101	51	3.1734747023809575
2202	1	2.7787388392857153
2202	2	0.34454985119047876
2202	3	0.5451822916666629
2202	4	1.369103422619041
2202	5	1.8888950892857252
2202	6	2.007533482142854
2202	7	2.2545014880952436
2202	8	3.2954799107142847
2202	9	2.068098958333337
2202	10	3.0391183035714278
2202	11	1.8702194940476247
2202	12	2.3110305059523952
2202	13	4.0809895833333485
2202	14	2.848809523809514
2202	15	3.9688430059523867
2202	16	3.3116071428571487
2202	17	3.5668898809523952
2202	18	2.4286272321428584
2202	19	2.3126116071428555
2202	20	3.0901971726190425
2202	21	4.0150111607142875
2202	22	2.914583333333347
2202	23	4.744084821428572
2202	24	3.129129464285711
2202	25	4.21184895833332
2202	26	2.903738839285708
2202	27	4.204892113095234
2202	28	4.776506696428584
2202	29	3.53463541666666
2202	30	4.548642113095241
2202	31	4.475892857142846
2202	32	4.1851562499999915
2202	33	3.1880580357142847
2202	34	3.9935825892857153
2202	35	3.073735119047626
2202	36	2.564732142857146
2202	37	4.859319196428572
2202	38	3.9438802083333258
2202	39	4.205617559523809
2202	40	4.373586309523816
2202	41	4.433612351190483
2202	42	4.087351190476198
2202	43	3.7262090773809646
2202	44	0.04583333333333428
2202	45	2.5132812499999986
2202	46	0.4895647321428598
2202	47	3.5169270833333357
2202	48	3.052046130952384
2202	49	1.2550223214285765
2202	50	4.784356398809514
2202	51	1.4591889880952422
1102	1	-0.0248325892857153
1102	2	0.16597842261904816
1102	3	1.2416108630952323
1102	4	1.5476748511904717
1102	5	2.0853236607142946
1102	6	-1.349609375
1102	7	0.593787202380959
1102	8	-0.2223772321428612
1102	9	-2.931901041666663
1102	10	2.253404017857143
1102	11	-0.7190662202380906
1102	12	0.6681733630952493
1102	13	2.0631324404762026
1102	14	1.4023809523809447
1102	15	1.2009858630952408
1102	16	1.6866071428571487
1102	17	1.4954613095238258
1102	18	3.464341517857143
1102	19	1.5626116071428555
1102	20	2.3044828869047578
1102	21	1.086439732142857
1102	22	2.8788690476190624
1102	23	-0.5594866071428584
1102	24	2.0934151785714263
1102	25	-2.395293898809534
1102	26	0.3144531249999929
1102	27	0.9370349702380878
1102	28	-0.2413504464285623
1102	29	2.6953497023809447
1102	30	0.2807849702380949
1102	31	0.24374999999999147
1102	32	1.488727678571422
1102	33	2.8487723214285694
1102	34	-0.3278459821428541
1102	35	0.23444940476191078
1102	36	-0.9888392857142847
1102	37	-3.1585379464285737
1102	38	0.7117373511904717
1102	39	0.6877604166666629
1102	40	-3.8049851190476147
1102	41	2.3800409226190524
1102	42	-0.8055059523809476
1102	43	4.19049479166668
1102	44	-0.04345238095238102
1102	45	0.6025669642857139
1102	46	-2.88543526785714
1102	47	-5.32235863095238
1102	48	1.0163318452380992
1102	49	-1.9056919642857082
1102	50	3.3914992559523682
1102	51	3.9234747023809575
2203	1	-5.524832589285715
2203	2	-1.0840215773809518
2203	3	0.3666108630952323
2203	4	0.17267485119047166
2203	5	-1.7896763392857054
2203	6	1.775390625
2203	7	1.468787202380959
2203	8	-2.222377232142861
2203	9	-4.181901041666663
2203	10	-1.371595982142857
2203	11	-1.9690662202380906
2203	12	2.2931733630952493
2203	13	-0.4368675595237974
2203	14	-0.47261904761905527
2203	15	-2.674014136904759
2203	16	-1.4383928571428513
2203	17	-0.7545386904761742
2203	18	1.589341517857143
2203	19	0.4376116071428555
2203	20	-3.9455171130952422
2203	21	1.211439732142857
2203	22	0.7538690476190624
2203	23	1.4405133928571416
2203	24	-0.03158482142857366
2203	25	1.604706101190466
2203	26	1.064453124999993
2203	27	-6.312965029761912
2203	28	-3.4913504464285623
2203	29	-3.5546502976190553
2203	30	0.7807849702380949
2203	31	-4.0062500000000085
2203	32	3.113727678571422
2203	33	3.7237723214285694
2203	34	3.172154017857146
2203	35	-0.6405505952380892
2203	36	-2.6138392857142847
2203	37	-0.40853794642857366
2203	38	0.7117373511904717
2203	39	-0.3122395833333371
2203	40	-1.0549851190476147
2203	41	-1.2449590773809476
2203	42	-0.4305059523809476
2203	43	2.56549479166668
2203	44	1.956547619047619
2203	45	-2.022433035714286
2203	46	-0.7604352678571402
2203	47	2.4276413690476204
2203	48	1.6413318452380992
2203	49	2.844308035714292
2203	50	3.8914992559523682
2203	51	2.5484747023809575
1103	1	-2.6498325892857153
1103	2	1.7909784226190482
1103	3	-1.2583891369047677
1103	4	-0.5773251488095283
1103	5	1.2103236607142946
1103	6	-2.849609375
1103	7	-0.031212797619041055
1103	8	0.5276227678571388
1103	9	2.068098958333337
1103	10	2.253404017857143
1103	11	1.9059337797619094
1103	12	1.9181733630952493
1103	13	0.06313244047620259
1103	14	-2.0976190476190553
1103	15	1.9509858630952408
1103	16	1.1866071428571487
1103	17	0.6204613095238258
1103	18	0.46434151785714306
1103	19	0.06261160714285552
1103	20	2.0544828869047578
1103	21	2.586439732142857
1103	22	2.8788690476190624
1103	23	3.3155133928571416
1103	24	3.3434151785714263
1103	25	-0.020293898809534028
1103	26	0.6894531249999929
1103	27	2.187034970238088
1103	28	3.1336495535714377
1103	29	0.44534970238094473
1103	30	-0.2192150297619051
1103	31	-3.6312500000000085
1103	32	0.11372767857142208
1103	33	-1.5262276785714306
1103	34	-4.702845982142854
1103	35	0.7344494047619108
1103	36	-1.8638392857142847
1103	37	-0.5335379464285737
1103	38	-0.5382626488095283
1103	39	-0.3122395833333371
1103	40	0.8200148809523853
1103	41	1.0050409226190524
1103	42	0.3194940476190524
1103	43	1.19049479166668
1103	44	-1.668452380952381
1103	45	1.9775669642857139
1103	46	-3.01043526785714
1103	47	0.1776413690476204
1103	48	-0.6086681547619008
1103	49	1.3443080357142918
1103	50	-0.23350074404763177
1103	51	0.9234747023809575
2204	1	1.064453125
2204	2	2.201692708333333
2204	3	-1.1691034226190524
2204	4	2.2262462797619023
2204	5	1.031752232142864
2204	6	0.8646763392857153
2204	7	1.2545014880952436
2204	8	3.2954799107142847
2204	9	2.6395275297619065
2204	10	3.3248325892857125
2204	11	1.1559337797619094
2204	12	1.59674479166668
2204	13	0.2238467261904873
2204	14	-0.7226190476190553
2204	15	3.683128720238095
2204	16	4.168750000000003
2204	17	3.5668898809523952
2204	18	0.28577008928571246
2204	19	3.741183035714286
2204	20	2.375911458333327
2204	21	3.1578683035714263
2204	22	3.4860119047619165
2204	23	1.4583705357142875
2204	24	1.271986607142857
2204	25	2.0689918154761813
2204	26	2.4751674107142776
2204	27	1.0620349702380878
2204	28	2.7765066964285836
2204	29	3.53463541666666
2204	30	0.1200706845238102
2204	31	4.475892857142846
2204	32	2.0422991071428527
2204	33	0.4737723214285694
2204	34	0.7078683035714306
2204	35	1.2165922619047649
2204	36	3.421875
2204	37	0.43074776785714164
2204	38	-1.198976934523813
2204	39	-0.7943824404761912
2204	40	-1.0549851190476147
2204	41	4.147898065476198
2204	42	3.230208333333337
2204	43	3.0119233630952493
2204	44	2.6172619047619037
2204	45	3.7989955357142833
2204	46	1.6324218750000057
2204	47	2.5169270833333357
2204	48	1.0520461309523839
2204	49	2.683593750000007
2204	50	2.070070684523799
2204	51	3.4591889880952422
2205	1	0.516834077380949
2205	2	0.9159784226190482
2205	3	-0.05005580357143202
2205	4	1.964341517857136
2205	5	2.4603236607142946
2205	6	3.150390625
2205	7	1.3021205357142946
2205	8	0.8192894345238031
2205	9	-5.098567708333331
2205	10	-0.07992931547619264
2205	11	0.32260044642857366
2205	12	-2.6651599702380864
2205	13	-1.8952008928571331
2205	14	-2.555952380952391
2205	15	1.6593191964285765
2205	16	1.1449404761904844
2205	17	-0.8378720238095099
2205	18	-2.6189918154761926
2205	19	-1.1873883928571445
2205	20	1.8044828869047578
2205	21	-2.5802269345238074
2205	22	-3.3711309523809376
2205	23	1.6488467261904773
2205	24	-0.823251488095238
2205	25	1.354706101190466
2205	26	2.189453124999993
2205	27	-1.1046316964285765
2205	28	-1.5330171130952266
2205	29	-0.012983630952390968
2205	30	2.7391183035714306
2205	31	1.1187499999999915
2205	32	-1.5529389880952422
2205	33	-0.6928943452380949
2205	34	-2.41117931547619
2205	35	0.8594494047619108
2205	36	-1.3638392857142847
2205	37	-1.1168712797619094
2205	38	-1.0799293154761926
2205	39	1.5627604166666629
2205	40	1.1116815476190496
2205	41	-1.8282924107142833
2205	42	-3.2221726190476154
2205	43	4.107161458333344
2205	44	-0.5017857142857167
2205	45	1.3942336309523782
2205	46	3.8228980654761955
2205	47	1.9693080357142847
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1211	27	-6.312965029761912
1211	28	-11.491350446428562
1211	29	-5.304650297619055
1211	30	-5.344215029761905
1211	31	-7.2562500000000085
1211	32	-1.886272321428578
1211	33	-4.651227678571431
1211	34	-0.2028459821428541
1211	35	-4.140550595238089
1211	36	-3.4888392857142847
1211	37	-1.0335379464285737
1211	38	-5.163262648809528
1211	39	-4.312239583333337
1211	40	-2.4299851190476147
1211	41	-2.2449590773809476
1211	42	-6.055505952380948
1211	43	-2.55950520833332
1211	44	-4.793452380952381
1211	45	-1.7724330357142861
1211	46	-0.7604352678571402
1211	47	-2.0723586309523796
1211	48	-1.3586681547619008
1211	49	-5.655691964285708
1211	50	0.39149925595236823
1211	51	-0.4515252976190425
1208	1	0.9215959821428541
1208	2	3.058835565476194
1208	3	2.402325148809517
1208	4	2.9405319940476176
1208	5	3.4603236607142946
1208	6	3.007533482142854
1208	7	2.5402157738095283
1208	8	2.152622767857139
1208	9	2.925241815476191
1208	10	1.3248325892857125
1208	11	-1.1297805059523753
1208	12	3.453887648809534
1208	13	0.3667038690476332
1208	14	-0.008333333333339965
1208	15	2.2545572916666714
1208	16	3.025892857142864
1208	17	-0.5759672619047436
1208	18	1.5714843749999972
1208	19	2.3126116071428555
1208	20	3.0901971726190425
1208	21	2.8721540178571416
1208	22	3.2002976190476318
1208	23	-3.8273437500000007
1208	24	2.8434151785714263
1208	25	3.9261346726190354
1208	26	2.903738839285708
1208	27	3.3477492559523725
1208	28	3.9193638392857224
1208	29	1.1060639880952294
1208	30	4.262927827380949
1208	31	4.761607142857137
1208	32	3.8994419642857068
1208	33	2.759486607142854
1208	34	3.7078683035714306
1208	35	2.5023065476190567
1208	36	3.1361607142857153
1208	37	4.57360491071428
1208	38	3.9438802083333258
1208	39	4.205617559523809
1208	40	2.6593005952381006
1208	41	2.7193266369047677
1208	42	2.0873511904761983
1208	43	3.44049479166668
1208	44	4.76011904761905
1208	45	3.5132812499999986
1208	46	4.346707589285714
1208	47	4.945498511904766
1208	48	4.623474702380953
1208	49	0.6835937500000071
1208	50	3.3557849702380835
1208	51	-1.255096726190473
1209	1	-2.506975446428573
1209	2	-3.798307291666667
1209	3	-3.8833891369047677
1209	4	1.369103422619041
1209	5	-0.968247767857136
1209	6	0.007533482142854098
1209	7	-2.4597842261904717
1209	8	-3.7045200892857153
1209	9	-4.646186755952378
1209	10	-4.246595982142857
1209	11	-4.272637648809518
1209	12	-1.546112351190466
1209	13	-2.490438988095228
1209	14	-0.15119047619048587
1209	15	-2.174014136904759
1209	16	-2.4026785714285666
1209	17	1.85260416666668
1209	18	-1.4285156250000028
1209	19	-1.5445312499999986
1209	20	-1.481231398809527
1209	21	-5.270703125000001
1209	22	-0.08541666666665293
1209	23	0.31551339285714164
1209	24	-0.15658482142857366
1209	25	1.9261346726190354
1209	26	-3.096261160714292
1209	27	1.6334635416666572
1209	28	2.205078125000007
1209	29	-0.8939360119047706
1209	30	0.4057849702380949
1209	31	-1.2383928571428626
1209	32	-3.2434151785714356
1209	33	-4.954799107142858
1209	34	-4.7207031249999964
1209	35	-2.06912202380952
1209	36	-9.006696428571427
1209	37	-0.28353794642857366
1209	38	-1.7704055059523824
1209	39	-2.3658110119047677
1209	40	-3.4835565476190418
1209	41	-4.709244791666663
1209	42	-3.9126488095238052
1209	43	-1.702362351190466
1209	44	-3.8113095238095234
1209	45	0.6561383928571445
1209	46	-0.6532924107142861
1209	47	-4.19735863095238
1209	48	1.6234747023809533
1209	49	-1.4592633928571388
1209	50	3.927213541666653
1209	51	1.6020461309523881
1214	1	1.9501674107142861
1214	2	1.5159784226190496
1214	3	1.7166108630952337
1214	4	-2.4023251488095276
1214	5	-2.5396763392857054
1214	6	0.9503906249999972
1214	7	1.968787202380959
1214	8	-2.4473772321428626
1214	9	0.26809895833333997
1214	10	1.953404017857146
1214	11	-0.04406622023809348
1214	12	-2.631826636904748
1214	13	2.1381324404762054
1214	14	-2.122619047619054
1214	15	-0.1740141369047592
1214	16	-1.2883928571428527
1214	17	-0.40453869047617275
1214	18	0.9143415178571459
1214	19	-0.2873883928571459
1214	20	-3.3955171130952415
1214	21	-3.21356026785714
1214	22	2.228869047619064
1214	23	2.915513392857143
1214	24	-3.9565848214285744
1214	25	4.154706101190463
1214	26	3.189453124999993
1214	27	2.662034970238089
1214	28	-0.5663504464285651
1214	29	-0.3796502976190581
1214	30	3.8057849702380935
1214	31	-3.3812500000000085
1214	32	-2.7862723214285765
1214	33	-2.72622767857143
1214	34	1.222154017857143
1214	35	2.5594494047619136
1214	36	-1.0638392857142875
1214	37	1.3164620535714278
1214	38	3.4867373511904702
1214	39	-1.5372395833333385
1214	40	1.9450148809523853
1214	41	-2.394959077380946
1214	42	-0.45550595238094616
1214	43	-7.359505208333321
1214	44	-3.8684523809523803
1214	45	-1.3724330357142875
1214	46	-7.310435267857141
1214	47	-3.1973586309523796
1214	48	2.5663318452380963
1214	49	-2.2306919642857075
1214	50	-1.3585007440476318
1214	51	-0.22652529761904105
1215	1	-2.816499255952383
1215	2	-2.2506882440476197
1215	3	-0.550055803571432
1215	4	1.7976748511904717
1215	5	1.4603236607142946
1215	6	-2.6829427083333357
1215	7	2.3021205357142946
1215	8	1.4859561011904745
1215	9	-2.098567708333327
1215	10	3.0867373511904788
1215	11	-2.6773995535714263
1215	12	-2.1651599702380864
1215	13	-1.8952008928571331
1215	14	1.6107142857142804
1215	15	-6.507347470238091
1215	16	-3.1883928571428513
1215	17	-3.8378720238095063
1215	18	0.7143415178571431
1215	19	-1.0207217261904802
1215	20	1.1378162202380935
1215	21	-1.913560267857143
1215	22	0.12886904761906237
1215	23	0.14884672619047734
1215	24	0.676748511904762
1215	25	-4.811960565476202
1215	26	-2.4772135416666714
1215	27	2.062034970238088
1215	28	-0.199683779761898
1215	29	1.487016369047609
1215	30	-3.760881696428573
1215	31	-4.047916666666676
1215	32	1.7803943452380864
1215	33	-2.6928943452380985
1215	34	-0.4111793154761898
1215	35	3.359449404761911
1215	36	3.1361607142857153
1215	37	-3.4502046130952415
1215	38	-4.079929315476196
1215	39	-4.937239583333337
1215	40	1.9450148809523853
1215	41	0.17170758928571672
1215	42	-0.8888392857142833
1215	43	-0.39283854166665577
1215	44	-0.16845238095238102
1215	45	0.3942336309523782
1215	46	-1.0104352678571402
1215	47	2.135974702380956
1215	48	-4.233668154761901
1215	49	-4.030691964285708
1215	50	-1.525167410714296
1215	51	1.3401413690476218
1212	1	1.3501674107142847
1212	2	0.9159784226190482
1212	3	3.1166108630952323
1212	4	1.7976748511904717
1212	5	1.4603236607142946
1212	6	-0.849609375
1212	7	0.13545386904762324
1212	8	-2.0140438988095255
1212	9	2.068098958333337
1212	10	0.9200706845238074
1212	11	2.1559337797619094
1212	12	-0.33182663690475067
1212	13	2.604799107142867
1212	14	-1.8892857142857196
1212	15	-2.507347470238095
1212	16	-8.855059523809519
1212	17	-1.8378720238095099
1212	18	-3.285658482142857
1212	19	0.4792782738095198
1212	20	1.8044828869047578
1212	21	0.5864397321428569
1212	22	-1.7044642857142733
1212	23	0.8155133928571416
1212	24	-1.6565848214285737
1212	25	-4.311960565476202
1212	26	-0.9772135416666714
1212	27	0.8953683035714235
1212	28	4.300316220238102
1212	29	1.3203497023809447
1212	30	-1.4275483630952408
1212	31	-4.3812500000000085
1212	32	1.9470610119047578
1212	33	0.9737723214285694
1212	34	-0.9111793154761898
1212	35	2.6927827380952465
1212	36	3.469494047619051
1212	37	-0.11687127976190936
1212	38	0.42007068452380736
1212	39	3.3960937499999986
1212	40	2.9450148809523853
1212	41	1.0050409226190524
1212	42	-2.8888392857142833
1212	43	-3.392838541666652
1212	44	-4.668452380952381
1212	45	0.3942336309523782
1212	46	0.9895647321428598
1212	47	1.135974702380956
1212	48	1.2663318452380992
1212	49	3.969308035714292
1212	50	2.974832589285704
1212	51	2.5068080357142932
1213	1	-1.3164992559523796
1213	2	-4.417354910714284
1213	3	0.449944196428568
1213	4	-0.20232514880952834
1213	5	1.4603236607142946
1213	6	-5.516276041666668
1213	7	-1.6978794642857054
1213	8	0.1526227678571388
1213	9	-2.931901041666663
1213	10	-15.913262648809525
1213	11	-1.8440662202380906
1213	12	-0.8318266369047507
1213	13	-5.39520089285713
1213	14	-1.3892857142857196
1213	15	0.8259858630952408
1213	16	-7.021726190476183
1213	17	-9.337872023809506
1213	18	0.7143415178571431
1213	19	2.3126116071428555
1213	20	1.471149553571422
1213	21	3.2531063988095212
1213	22	0.6288690476190624
1213	23	3.982180059523806
1213	24	-2.1565848214285737
1213	25	-6.311960565476202
1213	26	-5.143880208333339
1213	27	3.062034970238088
1213	28	-0.03301711309522659
1213	29	-3.846316964285723
1213	30	-4.927548363095237
1213	31	-4.047916666666676
1213	32	-3.0529389880952458
1213	33	-4.1928943452380985
1213	34	0.4221540178571459
1213	35	-4.307217261904757
1213	36	-0.8638392857142847
1213	37	1.7164620535714263
1213	38	-5.2465959821428605
1213	39	-7.270572916666669
1213	40	0.9450148809523853
1213	41	-5.32829241071428
1213	42	-0.7221726190476119
1213	43	-6.892838541666652
1213	44	-13.335119047619049
1213	45	-4.105766369047618
1213	46	-13.177101934523808
1213	47	-4.530691964285712
1213	48	0.09966517857143486
1213	49	-12.697358630952376
1213	50	-0.025167410714296068
1213	51	-12.49319196428571
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	4.0
22	12.0
23	19.0
24	30.0
25	33.0
26	88.0
27	91.0
28	127.0
29	133.0
30	175.0
31	195.0
32	218.0
33	233.0
34	257.0
35	307.0
36	385.0
37	531.0
38	711.0
39	593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.692010620323437	16.558049722423366	12.285783248853487	39.46415640839971
2	12.934362934362934	19.835907335907336	44.25675675675676	22.972972972972975
3	15.057915057915059	22.852316602316602	38.585907335907336	23.503861003861005
4	11.727799227799228	25.98938223938224	35.641891891891895	26.640926640926644
5	11.993243243243242	34.26640926640926	42.326254826254825	11.414092664092664
6	26.42374517374517	44.37741312741313	17.253861003861005	11.944980694980694
7	22.755791505791507	30.93629343629344	21.283783783783782	25.02413127413127
8	20.31853281853282	43.62934362934363	19.90830115830116	16.143822393822393
9	22.683397683397683	15.757722007722009	19.570463320463322	41.988416988416986
10	12.886100386100384	35.97972972972973	30.453667953667953	20.68050193050193
11	27.944015444015445	22.779922779922778	31.949806949806952	17.326254826254825
12	18.79826254826255	24.155405405405407	29.029922779922778	28.016409266409266
13	23.84169884169884	19.377413127413128	34.86969111969112	21.91119691119691
14	17.56756756756757	21.428571428571427	25.748069498069498	35.25579150579151
15	19.739382239382238	26.061776061776058	23.262548262548265	30.93629343629344
16	30.79150579150579	26.640926640926644	22.779922779922778	19.787644787644787
17	19.739382239382238	35.955598455598455	24.58976833976834	19.715250965250966
18	29.633204633204635	26.785714285714285	23.88996138996139	19.69111969111969
19	21.090733590733592	24.71042471042471	25.217181467181465	28.981660231660232
20	19.86003861003861	25.0	24.034749034749034	31.105212355212352
21	20.004826254826256	25.60328185328185	24.372586872586872	30.01930501930502
22	20.173745173745175	33.518339768339764	24.30019305019305	22.00772200772201
23	19.666988416988417	25.57915057915058	25.33783783783784	29.416023166023166
24	30.284749034749037	25.796332046332047	23.455598455598455	20.463320463320464
25	20.921814671814673	34.16988416988417	25.072393822393824	19.835907335907336
26	29.70559845559845	26.52027027027027	24.806949806949806	18.96718146718147
27	21.597490347490346	34.55598455598455	24.30019305019305	19.546332046332047
28	19.44980694980695	25.12065637065637	25.868725868725868	29.56081081081081
29	21.090733590733592	26.85810810810811	26.68918918918919	25.361969111969113
30	22.2972972972973	25.41023166023166	31.925675675675674	20.36679536679537
31	20.994208494208493	29.82625482625483	26.158301158301157	23.02123552123552
32	21.090733590733592	25.723938223938227	30.429536679536678	22.755791505791507
33	19.763513513513516	26.906370656370655	32.504826254826256	20.825289575289574
34	20.801158301158303	25.482625482625483	28.474903474903474	25.241312741312743
35	20.656370656370658	27.84749034749035	29.029922779922778	22.466216216216218
36	20.68050193050193	29.51254826254826	28.499034749034752	21.307915057915057
37	20.125482625482626	31.491312741312743	26.809845559845556	21.573359073359075
38	18.194980694980696	22.804054054054053	36.82432432432432	22.176640926640925
39	17.51930501930502	21.525096525096522	29.995173745173748	30.96042471042471
40	16.964285714285715	21.114864864864867	39.5511583011583	22.369691119691122
41	17.929536679536678	20.945945945945947	29.80212355212355	31.322393822393824
42	26.327220077220076	21.525096525096522	30.79150579150579	21.356177606177607
43	16.216216216216218	21.235521235521233	39.79247104247104	22.755791505791507
44	16.361003861003862	32.31177606177606	29.029922779922778	22.2972972972973
45	15.444015444015443	22.104247104247104	38.827220077220076	23.624517374517374
46	24.372586872586872	22.442084942084943	30.284749034749037	22.90057915057915
47	14.792471042471043	22.03185328185328	29.777992277992276	33.397683397683394
48	14.985521235521235	21.83880308880309	30.550193050193048	32.625482625482626
49	22.2972972972973	21.211389961389962	29.777992277992276	26.71332046332046
50	12.982625482625481	23.43146718146718	38.56177606177606	25.02413127413127
51	13.36872586872587	21.79054054054054	29.68146718146718	35.15926640926641
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	1.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	3.5
24	6.0
25	7.5
26	18.5
27	28.0
28	49.0
29	70.0
30	82.5
31	95.0
32	141.0
33	187.0
34	210.0
35	233.0
36	263.0
37	293.0
38	287.0
39	281.0
40	303.5
41	326.0
42	324.5
43	323.0
44	328.0
45	333.0
46	359.5
47	386.0
48	463.0
49	540.0
50	437.5
51	335.0
52	285.0
53	235.0
54	196.5
55	158.0
56	123.5
57	89.0
58	80.0
59	71.0
60	56.5
61	42.0
62	37.5
63	33.0
64	31.5
65	30.0
66	23.0
67	16.0
68	11.5
69	7.0
70	7.0
71	7.0
72	5.5
73	4.0
74	2.5
75	1.0
76	1.0
77	0.5
78	0.0
79	1.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02413127413127413
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4144.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.9507722007722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.6468559299549	89.72007722007721
2	0.8490315733616343	1.5444015444015444
3	0.10612894667020428	0.28957528957528955
4	0.07959671000265323	0.28957528957528955
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.05306447333510214	0.4343629343629344
>10	0.21225789334040857	3.2577220077220073
>50	0.02653223666755107	1.2548262548262548
>100	0.02653223666755107	3.209459459459459
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC	133	3.209459459459459	RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	52	1.2548262548262548	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	26	0.6274131274131274	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	22	0.5308880308880308	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC	18	0.4343629343629344	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	18	0.4343629343629344	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	16	0.3861003861003861	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	14	0.33783783783783783	RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	11	0.2654440154440154	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC	10	0.2413127413127413	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT	9	0.2171814671814672	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	9	0.2171814671814672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.02413127413127413
5	0.0	0.0	0.02413127413127413
6	0.0	0.0	0.02413127413127413
7	0.0	0.0	0.02413127413127413
8	0.0	0.0	0.02413127413127413
9	0.0	0.0	0.02413127413127413
10	0.0	0.0	0.02413127413127413
11	0.0	0.0	0.02413127413127413
12	0.0	0.0	0.02413127413127413
13	0.0	0.0	0.02413127413127413
14	0.0	0.0	0.02413127413127413
15	0.0	0.0	0.02413127413127413
16	0.0	0.0	0.02413127413127413
17	0.0	0.0	0.02413127413127413
18	0.0	0.0	0.02413127413127413
19	0.0	0.0	0.02413127413127413
20	0.0	0.0	0.02413127413127413
21	0.0	0.0	0.02413127413127413
22	0.0	0.0	0.02413127413127413
23	0.0	0.0	0.02413127413127413
24	0.0	0.0	0.07239382239382239
25	0.0	0.0	0.07239382239382239
26	0.0	0.0	0.12065637065637065
27	0.0	0.0	0.19305019305019305
28	0.0	0.0	0.36196911196911197
29	0.0	0.0	0.7480694980694981
30	0.0	0.0	1.1583011583011582
31	0.0	0.0	2.195945945945946
32	0.0	0.0	3.7644787644787643
33	0.0	0.0	5.3812741312741315
34	0.0	0.0	6.66023166023166
35	0.0	0.0	8.445945945945946
36	0.0	0.0	10.014478764478765
37	0.0	0.0	11.751930501930502
38	0.0	0.0	13.996138996138995
39	0.0	0.0	16.457528957528957
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATTC	20	6.2420196E-4	45.000004	28
AAATCTC	30	1.6824488E-6	45.000004	35
TTCAAAT	20	6.2420196E-4	45.000004	32
TCAAATC	20	6.2420196E-4	45.000004	33
TATTCAA	20	6.2420196E-4	45.000004	30
AGACCTA	20	6.2420196E-4	45.000004	25
ACCTATT	20	6.2420196E-4	45.000004	27
ATTCAAA	20	6.2420196E-4	45.000004	31
CTATTCA	20	6.2420196E-4	45.000004	29
AGCCCAC	50	1.0913936E-11	45.0	17
AATCTCG	35	8.644747E-8	45.0	36
ATCTCCG	50	1.0913936E-11	45.0	10
GCCCACG	50	1.0913936E-11	45.0	18
GAGCCCA	50	1.0913936E-11	45.0	16
CACGAGA	50	1.0913936E-11	45.0	21
ACGAGAC	50	1.0913936E-11	45.0	22
CGAGCCC	50	1.0913936E-11	45.0	15
CCGAGCC	50	1.0913936E-11	45.0	14
TCTCCGA	50	1.0913936E-11	45.0	11
CGAGACC	25	3.2510856E-5	45.0	23
>>END_MODULE