FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_38.34100000005137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_38.34100000005137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4144
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC1333.209459459459459RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC521.2548262548262548RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC260.6274131274131274No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC220.5308880308880308No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC180.4343629343629344No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC180.4343629343629344No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC160.3861003861003861No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC140.33783783783783783RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC110.2654440154440154No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC100.2413127413127413No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT90.2171814671814672No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC90.2171814671814672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTC206.2420196E-445.00000428
AAATCTC301.6824488E-645.00000435
TTCAAAT206.2420196E-445.00000432
TCAAATC206.2420196E-445.00000433
TATTCAA206.2420196E-445.00000430
AGACCTA206.2420196E-445.00000425
ACCTATT206.2420196E-445.00000427
ATTCAAA206.2420196E-445.00000431
CTATTCA206.2420196E-445.00000429
AGCCCAC501.0913936E-1145.017
AATCTCG358.644747E-845.036
ATCTCCG501.0913936E-1145.010
GCCCACG501.0913936E-1145.018
GAGCCCA501.0913936E-1145.016
CACGAGA501.0913936E-1145.021
ACGAGAC501.0913936E-1145.022
CGAGCCC501.0913936E-1145.015
CCGAGCC501.0913936E-1145.014
TCTCCGA501.0913936E-1145.011
CGAGACC253.2510856E-545.023
Produced by FastQC (version 0.11.2)